Hubungan Karakteristik Ibu Hamil Trimester III Dengan Tingkat Kecemasan Dalam Menghadapi Persalinan Di Poli Kia Puskesmas Tuminting

: Anxiety (Anxiety) is a fear that is not clear and is not supported by the circumstances. Pregnancy can be a source of anxiety stressor, Pregnancy classified into the first trimester, second trimester and third trimester. Anxiety experienced by pregnant women were divided into categories of types of pregnancy gravidity, age, and education level. The purpose of the study to determine the relationship of the characteristics of third trimester pregnant women with levels of anxiety in facing childbirth. Design research uses descriptive analytical method using a cross-sectional study design (cross-sectional study). The sample of 60 people. Analysis of the data using chi-square test (X2), the 95% significance level (α ≤ 0.05) showed the value of p= 0,017. Conclusion no relationship characteristics third trimester pregnant women with levels of anxiety in facing childbirth at Poly KIA public health of Tuminting. Advice for health care workers is expected to increase consulting services for pregnant women to be able to cope with anxiety in the face of labor

Hubungan Kebiasaan Merokok Di Dalam Rumah Dengan Kejadian Ispa Pada Anak Umur 1-5 Tahun Di Puskesmas Sario Kota Manado

ISPA (Infeksi Saluran Pernapasan Akut) akan terjadi apabila kekebalan tubuh menurun.Beberapa upaya dapat dilakukan untuk menurunkan resiko penyakit ISPA, antara lain denganmenghilangkan kebiasaan merokok di dalam rumah. Kejadian ISPA pada anak di Puskesmas SarioKota Manado menduduki peringkat pertama diantara 10 penyakit yang paling menonjol. Tujuanpenelitian ini untuk mengidentifikasi kebiasaan merokok di dalam rumah dan kejadian ISPA sertauntuk menganalisis hubungan antara kebiasaan merokok dengan kejadian ISPA.Desain penelitianyang digunakan adalah desain Cross Sectional dan data dikumpulkan dari responden menggunakanlembar kuisioner.Sampel pada penelitian ini berjumlah 51 responden yang didapat menggunakanteknik consecutive sampling.Hasil penelitianuji statistik menggunakan uji chi-square pada tingkatkemaknaan 95% (α ≤ 0,05),maka didapatkan nilai p= 0,002. Ini berarti bahwa nilai p< α(0,05).Kesimpulan dalam penelitian ini ada hubungan antara kebiasaan merokok dengan kejadianISPA pada anak. Rekomendasi untuk peneliti selanjutnya diharapkan dapat meneliti mengenaifaktor-faktor lain seperti Ventilasi Rumah, Kepadatan Hunian, Status sosioekonomi yang dapatmenyebabkan penyakit ISPA

Hubungan Status Gizi Dengan Sindrom Pre Menstruasi Pada Remaja Putri Di SMA Frater Don Bosco Manado

: Pre Menstrual Syndrome is a collection of physical symptoms, psychological and emotional associated with a woman's menstrual cycle. The symptoms are expected and usually occur regularly on a two-week period before menstruation. The aim of research to determine the relationship of nutritional status with pre menstrual syndrome in young women at Frater Don Bosco Senior High School Manado. Design research is an analytic survey with cross sectional approach. This research was conducted in Frater Don Bosco Senior High School Manado on June 1th – 19th 2015. The sample in this research is purposive sampling with 60 respondents were included in the inclusion criteria. The instrument that uses questionnaires and observation sheets. The results of the statistical test using computerized systems by using the chi-square test (X2) at the level of significance 95% (α = 0,05). Statistical analysis showed p value = 0,000 with level of significance <0,05. Conclusion there is a meaningful correlation between nutritional status and pre menstrual syndrome in young women at Frater Don Bosco Senior High School Manado. Advice for young women to better maintain the nutritional status to reduce the risk of having symptoms of pre-menstrual syndrome heavier that can interfere daily activities

Perbandingan Kualitas Hidup Pasien Gagal Ginjal Kronik Dengan Comorbid Faktor Diabetes Melitus Dan Hipertensi Di Ruangan Hemodialisa Rsup. Prof. Dr. R. D. Kandou Manado

: Chronic kidney disease is a disease that causes the function of kidney organs to decrease until it is finally unable to does its function properly. Life quality is a conceptual model that aims to describe the client's perspective with a variety of terms. Therefore, the perspective of this life quality will be different among sick and healthy peoples. The purpose of this study was to find out the life quality of patients with chronic kidney disease with comorbid factors of diabetes mellitus and hypertension at Hemodialysis room of RSUP. Prof. Dr. R. D. Kandou Manado. The method used in this research is the analytic observational with cross sectional design. Sampling technique in this research is saturated sampling with 60 amount of sample. Data processing using computer program by using chi-square test with significance level 95% (α = 0,05). The result showed that the number of respondents of chronic kidney disease with comorbid hypertension have good life quality as many as 29 respondents (96,7%) and who have poor life quality as much as 1 respondent (3,3%). Whereas for chronic kidney disease patients with comorbid diabetes mellitus who have good life quality as many as 13 respondents (43.4%) and who have poor life quality as much as 17 respondents (56.7%) and obtained p value = 0.000. This conclusion shows a comparison of life quality between patients with chronic kidney disease with comorbid hypertension and diabetes mellitus

Hubungan Kebiasaan Merokok Dengan Kejadian Tuberkulosis Paru Di Puskesmas Siloam Kecamatan Tamako Kabupaten Kepulauan Sangihe

: Tuberculosis is contagious infection diseases caused by mycobacterium tuberculosis. Observation from siloam health center district of Tamako in Sangihe Island regency is most patients with pulmonary tuberculosis. Male sex who has the habit of smoking. The purpose of this research to determine the relationship of smoking and the incidence of pulmonary tuberculosis in siloam health center district of Tamako Sangihe Island. Method in this research used analytic survey crosssectional approach. This research was conducted at district of Tamako in October 2014 to January 2015.The samples in this research amounted to 30 people. Sample were taken used total sampling. Data obtained used the questioner and observation sheets. The data were abalysed used univariate and bivariate analysis includes chi square test with. The result of this research showed the values of p=0,01 which means that the p-value is smaller than the value of α=0,05. Conclution that there is a significant relationship between smoking and incidence of pulmonary tuberculosis.Suggested for futher research are expected to further investigate other factors that may cause the incidence of pulmonary tuberculosis

Long-Term Estrogen Receptor Beta Agonist Treatment Modifies the Hippocampal Transcriptome in Middle-Aged Ovariectomized Rats.

Estradiol (E2) robustly activates transcription of a broad array of genes in the hippocampal formation of middle-aged ovariectomized rats via estrogen receptors (ERalpha, ERbeta, and G protein-coupled ER). Selective ERbeta agonists also influence hippocampal functions, although their downstream molecular targets and mechanisms are not known. In this study, we explored the effects of long-term treatment with ERbeta agonist diarylpropionitrile (DPN, 0.05 mg/kg/day, sc.) on the hippocampal transcriptome in ovariectomized, middle-aged (13 month) rats. Isolated hippocampal formations were analyzed by Affymetrix oligonucleotide microarray and quantitative real-time PCR. Four hundred ninety-seven genes fulfilled the absolute fold change higher than 2 (FC > 2) selection criterion. Among them 370 genes were activated. Pathway analysis identified terms including glutamatergic and cholinergic synapse, RNA transport, endocytosis, thyroid hormone signaling, RNA degradation, retrograde endocannabinoid signaling, and mRNA surveillance. PCR studies showed transcriptional regulation of 58 genes encoding growth factors (Igf2, Igfb2, Igf1r, Fgf1, Mdk, Ntf3, Bdnf), transcription factors (Otx2, Msx1), potassium channels (Kcne2), neuropeptides (Cck, Pdyn), peptide receptors (Crhr2, Oprm1, Gnrhr, Galr2, Sstr1, Sstr3), neurotransmitter receptors (Htr1a, Htr2c, Htr2a, Gria2, Gria3, Grm5, Gabra1, Chrm5, Adrb1), and vesicular neurotransmitter transporters (Slc32a1, Slc17a7). Protein-protein interaction analysis revealed networking of clusters associated with the regulation of growth/troph factor signaling, transcription, translation, neurotransmitter and neurohormone signaling mechanisms and potassium channels. Collectively, the results reveal the contribution of ERbeta-mediated processes to the regulation of transcription, translation, neurogenesis, neuromodulation, and neuroprotection in the hippocampal formation of ovariectomized, middle-aged rats and elucidate regulatory channels responsible for DPN-altered functional patterns. These findings support the notion that selective activation of ERbeta may be a viable approach for treating the neural symptoms of E2 deficiency in menopause

Increasing rabies data availability: the example of a One Health research project in Chad, Côte d'Ivoire and Mali

Rabies is a fatal but preventable disease that remains notoriously underreported. Weak data availability hampers advocacy, constitutes a barrier to resource allocation and inhibits effective prevention and control. To gain better insight into the global rabies burden and human vaccine demand several studies were funded through the Vaccine Alliance (GAVI) learning agenda. With the help of this funding, Swiss TPH and local in country partner organizations implemented a One Health research project in Chad, Cote d'Ivoire and Mali to collect data at household, public health facility and veterinary level. This paper describes the implementation of this research project and evaluates its success on amount of information gained, achieved capacity building, impact on knowledge creation and influence on national and international policies. The project was based on the One Health concept and guided by the principles of transboundary research partnerships formulated by the Swiss Academy of Sciences. Data was collected on bite incidents and health seeking from over 24'000 households, on access to treatment of over 8'800 bite cases registered in public health facilities and on the status of over 1'800 rabies suspect animals. Selected country specific datasets have contributed to more than 10 scientific articles so far. On the international level, the multi-level data collection provided a unique set of indicators to inform, along with results from other studies, new WHO rabies immunization recommendations and a vaccine investment case scenario to prevent human rabies. New rabies burden estimates based on the data gathered are published for Mali and will be modelled for the whole West and Central African region. On the national level, the project facilitated communication between animal health and human health workers catalyzing creation of local and national committees and formulation of national action plans for Mali and Cote D'Ivoire. Major challenges arose from lack of data collection and documentation experience of human health and veterinary workers and weak infrastructural capacities of the veterinary and human health systems of the project countries. Through adherence to the principles of transboundary research partnerships, project team members acquired valuable research and networking skills despite language barriers, enabling them to play key roles in the future agenda towards national, regional and global canine rabies elimination. Project external collaborations with local public institutions was facilitated through long-term local partnerships. Both factors enabled success in project implementation and outcomes by identifying and mitigating risks in advance, resolving challenges amiably and enabling mutual knowledge creation as a fructuous ground for sustained commitment. Lack of immediate follow-up funding did not allow to maintain activities beyond the project timeframe. However, the national and international policy changes triggered, as well as the strengthened local disease control and research capacities provides sustainable basis for the elimination of dog transmitted human rabies

From reverse innovation to global innovation in animal health: a review

Reverse innovation refers to learning from or diffusion of innovations developed in low income settings and further translated to industrialized countries. There is lack of consensus regarding terminology, but the idea that innovations in low-income countries are promising for adoption in high-income contexts is not new. However, in healthcare literature globally, the vast majority of publications referring to 'disruptive innovation' were published in the last ten years. To assess the potential of innovative developments and technologies for improving animal health, we initiated a literature review in 2020. We used a combined approach, incorporating targeted searching in PubMed using a key word algorithm with a snowball technique, to identify 120 relevant publications and extract data for qualitative coding. Heterogeneity of articles precluded meta-analysis, quality scoring and risk of bias analysis. We can distinguish technical innovations like new digital devices, diagnostic tests and procedures, and social innovations of intersectoral cooperation. We profile two case studies to describe potential global innovations: an integrated surveillance and response system in Somali Regional State, Ethiopia and a blockchain secured One Health intervention to optimally provide post-exposure prophylaxis for rabies exposed people in West Africa. Innovation follows no borders and can also occur in low-income settings, under constraints of cost, lack of services and infrastructure. Lower administrative and legal barriers may contribute to produce innovations that would not be possible under conditions of high density of regulation. We recommend using the term global innovation, which highlights those emanating from international partnership to solve problems of global implications

Output from VIP cells of the mammalian central clock regulates daily physiological rhythms

The suprachiasmatic nucleus (SCN) circadian clock is critical for optimising daily cycles in mammalian physiology and behaviour. The roles of the various SCN cell types in communicating timing information to downstream physiological systems remain incompletely understood, however. In particular, while vasoactive intestinal polypeptide (VIP) signalling is essential for SCN function and whole animal circadian rhythmicity, the specific contributions of VIP cell output to physiological control remains uncertain. Here we reveal a key role for SCN VIP cells in central clock output. Using multielectrode recording and optogenetic manipulations, we show that VIP neurons provide coordinated daily waves of GABAergic input to target cells across the paraventricular hypothalamus and ventral thalamus, supressing their activity during the mid to late day. Using chemogenetic manipulation, we further demonstrate specific roles for this circuitry in the daily control of heart rate and corticosterone secretion, collectively establishing SCN VIP cells as influential regulators of physiological timing