Cloning of the β-phycocyanin gene from Anacystis nidulans

AbstractThe β-phycocyanin gene, pcyB, of Anacystis nidulans was isolated from an Escherichia coli λ-phage bank by the use of synthetic oligonucleotides derived from the 170 amino acid sequence of the β-phycocyanin protein. Two positive, overlapping λ-clones were found. Partial DNA sequencing of one of the clones gave a deduced amino acid sequence which was in full agreement with a portion of the published sequence of A. nidulans β-phycocyanin. A comparison with the published DNA sequence for β-phycocyanin from Agmenellum quadruplicatum shows a DNA sequence homology of 70.4% over the sequenced region