Search and tracking method of cloud fields on image

In work the method and algorithm of data use for passive sounding of the earth (satellite remote sensing) are offered for estimation of speed and direction of wind. Presented the method of object search and tracking of the cloud fields in images

Comparison of the effectiveness of optic atmosphere investigation tasks distribution techniques in local network

The research introduces a comparative review of atmosphere investigation tasks distribution techniques in local network. The article provides information about algorithms, simulation modeling methods and the results of the modeling. © (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only

Dinuclear Platinum Complexes as potential anticancer drugs : insights in the intracellular distribution

This thesis deals with various aspects of intracellular behavior of antitumor-active dinuclear platinum complexes, i.e. their anticancer activity, cellular distribution and nephrotoxicity. A major part of this thesis is focused on the investigation of cellular processing of dinuclear platinum anticancer drugs using fluorescence microscopy. Besides, design of new dinuclear platinum anticancer compounds is described, the relationships between the structure and nephrotoxicity of dinuclear complexes are discussed, and application of dinuclear platinum complexes in drug targeting is evaluated.LEI Universiteit LeidenCoördinatie bio-anorganische chemi

Economic Ecosystems and Their Classification

The most important trends in modern social development are economic convergence and digitalization. It is their interaction that creates new opportunities for increasing competitiveness and efficiency in the framework of joint business of representatives of various segments and sectors of the economy. The main institutional and organizational form of doing business within the framework of intersectoral economic convergence under the influence of digitalization is becoming an ecosystem. The aim of the article is to clarify the concept of an ecosystem as a form of joint business in the context of intersectoral economic convergence and digitalization, as well as to build a classification of ecosystems. The study puts forward the following hypothesis the ecosystem as an institutional and organizational form of running a joint business is the result of the simultaneous influence of intersectoral economic convergence and digitalization on it, and “the connection of the basic product provided to the client by the initiator of the intersectoral economic convergence before the creation of the ecosystem with digital and/or information technology ”can be used for the economic ecosystems classification. The novelty of the approach is the following. Considering an ecosystem as a form of joint business running with the simultaneous impact of economic intersectoral convergence and digitalization on it makes it possible to clarify the concept of an ecosystem, highlight as the ecosystem parameter used to the ecosystem classify and as its main characteristic, the values of which can be used to construct a classification of economic ecosystems

Model of Effective Business Formation

The task of improving business performance always raises the problem of choosing the most effective ways to ensure it. The article is devoted to the substantiation of the business efficiency criterion, in accordance with the requirements of which those areas of business development that provide it with the greatest efficiency should be determined. The article puts forward and confirms the hypothesis that, according to the selected criterion the greatest efficiency can be provided not only by the variant of the simultaneous implementation of all possible directions for increasing efficiency, but also by the variant of their implementation in parts — in the form of sets of directions. The authors propose a model for the formation of an effective business, using Pareto optimality and implemented on the basis of two developed Algorithms, which is illustrated by a numerical example

Non-Phosphorylatable PEA-15 Sensitises SKOV-3 Ovarian Cancer Cells to Cisplatin

The efficacy of cisplatin-based chemotherapy in ovarian cancer is often limited by the development of drug resistance. In most ovarian cancer cells, cisplatin activates extracellular signal-regulated kinase1/2 (ERK1/2) signalling. Phosphoprotein enriched in astrocytes (PEA-15) is a ubiquitously expressed protein, capable of sequestering ERK1/2 in the cytoplasm and inhibiting cell proliferation. This and other functions of PEA-15 are regulated by its phosphorylation status. In this study, the relevance of PEA-15 phosphorylation state for cisplatin sensitivity of ovarian carcinoma cells was examined. The results of MTT-assays indicated that overexpression of PEA-15AA (a non-phosphorylatable variant) sensitised SKOV-3 cells to cisplatin. Phosphomimetic PEA-15DD did not affect cell sensitivity to the drug. While PEA-15DD facilitates nuclear translocation of activated ERK1/2, PEA-15AA acts to sequester the kinase in the cytoplasm as shown by Western blot. Microarray data indicated deregulation of thirteen genes in PEA-15AA-transfected cells compared to non-transfected or PEA-15DD-transfected variants. Data derived from The Cancer Genome Atlas (TCGA) showed that the expression of seven of these genes including EGR1 (early growth response protein 1) and FLNA (filamin A) significantly correlated with the therapy outcome in cisplatin-treated cancer patients. Further analysis indicated the relevance of nuclear factor erythroid 2-related factor 2/antioxidant response element (Nrf2/ARE) signalling for the favourable effect of PEA-15AA on cisplatin sensitivity. The results warrant further evaluation of the PEA-15 phosphorylation status as a potential candidate biomarker of response to cisplatin-based chemotherapy. View Full-Tex

Altered localisation of the copper efflux transporters ATP7A and ATP7B associated with cisplatin resistance in human ovarian carcinoma cells

Metals in Catalysis, Biomimetics & Inorganic Material

Key Players of Cisplatin Resistance: Towards a Systems Pharmacology Approach

The major obstacle in the clinical use of the antitumor drug cisplatin is inherent and acquired resistance. Typically, cisplatin resistance is not restricted to a single mechanism demanding for a systems pharmacology approach to understand a whole cell’s reaction to the drug. In this study, the cellular transcriptome of untreated and cisplatin-treated A549 non-small cell lung cancer cells and their cisplatin-resistant sub-line A549rCDDP2000 was screened with a whole genome array for relevant gene candidates. By combining statistical methods with available gene annotations and without a previously defined hypothesis HRas, MAPK14 (p38), CCL2, DOK1 and PTK2B were identified as genes possibly relevant for cisplatin resistance. These and related genes were further validated on transcriptome (qRT-PCR) and proteome (Western blot) level to select candidates contributing to resistance. HRas, p38, CCL2, DOK1, PTK2B and JNK3 were integrated into a model of resistance-associated signalling alterations describing differential gene and protein expression between cisplatin-sensitive and -resistant cells in reaction to cisplatin exposure