Comparison of Three Methods for Diagnosis of Cutaneous Leishmaniasis

Background: Leishmaniasis is one of the infectious parasitic diseases of highest incidence in the world. Cutaneous Leishmaniasis (CL) has long been reported in Shiraz, Southern Iran. There is a need to find a sensitive and specific method for treatment and control of the disease.Methods: We have compared the sensitivity of the conventional methods microscopy and cultiva­tion of lesion scrapes against PCR amplification of parasite kinetoplast DNA from these sam­ples. The samples (n=219) were obtained from the patients clinically suspected of CL. The smears were stained with Giemsa for microscopy and cultured in Novy-Nicolle-McNeal (NNN) blood agar for promastigote growth. For PCR, the dry smears were scraped off the slides and DNA was extracted.Results: The positive rates from 219 specimens were 76.71%, 50.68%, and 93.61% for micros­copy, cultivation, and PCR, respectively. The highest correlation was found between PCR and micros­copy method (P= 0.014). In PCR assay, 95.61%, 3.9%, and 0.49% of the samples were identi­fied as Leishmania major, L. tropica, and dermatropic L. infantum, respectively.Conclusion: The PCR method appears to be the most sensitive for the diagnosis of CL and is valu­able for identifying the other species of Leishmania with confusing dermatropic signs

Proteomic analysis of tyrosine phosphorylation during human liver transplantation

BACKGROUND: Ischemia-reperfusion (I/R) causes a dramatic reprogramming of cell metabolism during liver transplantation and can be linked to an alteration of the phosphorylation level of several cellular proteins. Over the past two decades, it became clear that tyrosine phosphorylation plays a pivotal role in a variety of important signalling pathways and was linked to a wide spectrum of diseases. Functional profiling of the tyrosine phosphoproteome during liver transplantation is therefore of great biological significance and is likely to lead to the identification of novel targets for drug discovery and provide a basis for novel therapeutic strategies. RESULTS: Using liver biopsies collected during the early phases of organ procurement and transplantation, we aimed at characterizing the global patterns of tyrosine phosphorylation during hepatic I/R. A proteomic approach, based on the purification of tyrosine phosphorylated proteins followed by their identification using mass spectrometry, allowed us to identify Nck-1, a SH(2)/SH(3 )adaptor, as a potential regulator of I/R injury. Using immunoblot, cell fractionation and immunohistochemistry, we demonstrate that Nck-1 phosphorylation, expression and localization were affected in liver tissue upon I/R. In addition, mass spectrometry identification of Nck-1 binding partners during the course of the transplantation also suggested a dynamic interaction between Nck-1 and actin during I/R. CONCLUSION: Taken together, our data suggest that Nck-1 may play a role in I/R-induced actin reorganization, which was previously reported to be detrimental for the hepatocytes of the transplanted graft. Nck-1 could therefore represent a target of choice for the design of new organ preservation strategies, which could consequently help to reduce post-reperfusion liver damages and improve transplantation outcomes

Genetic control of protein, oil and fatty acids content under partial drought stress and late sowing conditions in sunflower (Helianthus annuus)

The purpose of the present study was to map quantitative trait locus (QTLs) associated with percentage of seed protein, oil and fatty acids content under different conditions in a population of recombinant inbred lines (RILs) of sunflower. Three independent field experiments were conducted with well-, partial-irrigated and late-sowing conditions in randomized complete block design with three replications. High significant variation among genotypes is observed for the studied traits in all conditions. Several specific and non-specific QTLs for the aforementioned traits were detected. Under late-sowing condition, a specific QTL of palmitic acid content on linkage group 6 (PAC-LS.6) is located between ORS1233 and SSL66_1 markers. Common chromosomic regions are observed for percentage of seed oil and stearic acid content on linkage group 10 (PSO-PI.10 and SAC-WI.10) and 15 (PSO-PI.15 and SAC-LS.15). Overlapping occurs for QTLs of oleic and linoleic acids content on linkage groups 10, 11 and 16. Seven QTLs associated with palmitic, stearic, oleic and linoleic acids content are identified on linkage group 14. These common QTLs are linked to HPPD homologue, HuCL04260C001. Coincidence of the position for some detected QTLs and candidate genes involved in enzymatic and non-enzymatic antioxidants would be useful for the function of the respective genes in fatty acid stability.Key words: Sunflower, quantitative trait locus, simple sequence repeats, oil content, protein content, fatty acids

USING SMOS PASSIVE MICROWAVE DATA TO DEVELOP SMAP FREEZE/THAW ALGORITHMS ADAPTED FOR THE CANADIAN SUBARCTIC

Seasonal terrestrial Freeze/Thaw cycle in Northern Quebec Tundra (Nunavik) was determined and evaluated with passive microwave observations. SMOS time series data were analyzed to examine seasonal variations of soil freezing, and to assess the impact of land cover on the Freeze/Thaw cycle. Furthermore, the soil freezing maps derived from SMOS observations were compared to field survey data in the region near Umiujaq. The objective is to develop algorithms to follow the seasonal cycle of freezing and thawing of the soil adapted to Canadian subarctic, a territory with a high complexity of land cover (vegetation, soil, and water bodies). Field data shows that soil freezing and thawing dates vary much spatially at the local scale in the Boreal Forest and the Tundra. The results showed a satisfactory pixel by pixel mapping for the daily soil state monitoring with a > 80% success rate with in situ data for the HH and VV polarizations, and for different land cover. The average accuracies are 80% and 84% for the soil freeze period, and soil thaw period respectively. The comparison is limited because of the small number of validation pixels

Flexible piezoresistive pressure sensors for smart textiles

The development of smart textiles relies in many applications on of textile-integrated sensors. Flexible piezoresistive pressure sensors have many potential applications, for instance in sports science. In this study, flexible pressure sensors are built using piezoresistive polymer film and conductive fabric. Tests using a universal testing machine show that the sensors are functional, accurate, although showing some hysteresis. However, methods for joining the electrode and piezoresistive layers are necessary to assure mechanical stability of the sensor, without affecting electrical contact between layers. Several methods were tested and results are reported here. The use of thermofusible bonding nets and webs has been found to be an interesting solution.This work is financed by Project “Deus ex Machina”, NORTE-01-0145-FEDER-000026, funded by CCDRN, through Sistema de Apoio à Investigação Cientifica e Tecnológica (Projetos Estruturados I&D&I) of Programa Operacional Regional do Norte, from Portugal 2020 and by FEDER funds through the Competitivity Factors Operational Programme - COMPETE and by national funds through FCT – Foundation for Science and Technology within the scope of the project POCI-01-0145-FEDER-007136. Derya Tama thanks FCT for fellowship 2C2T-BPD-08-2017.info:eu-repo/semantics/publishedVersio

Comparison of Knee and Hip Kinematics during Landing and Cutting between Elite Male Football and Futsal Players

To design an accurate sport injury prevention program, alterations in the knee and hip kinematic variables involved in injury mechanisms should be known. The main purpose of the current study was to compare knee and hip kinematic variables during landing and cutting among male football and futsal players, and to discuss them within an injury description frame. Twenty football (20.5 ± 2.1 years., 74.5 ± 6.9 kg and 1.79 ± 0.07 m) and twenty futsal players (20.3 ± 2.0 years., 73.5 ± 7.1 kg and 1.78 ± 0.07 m), with at least three years’ experience of playing in the Kerman Province League, participated in this study. Hip flexion, knee flexion and knee valgus angle during two main movements with risk of injury, such as landing and cutting, were measured using a motion capture system with passive markers at 120-Hz sampling frequency. Landing and cutting maneuvers were administered in as natural way as possible. Results showed significant differences in landing and cutting maneuvers between groups in hip flexion, knee flexion and knee valgus angle. Results indicated that footballers have less extension of hip and knee joints than futsal players in landing maneuvers, which may be due to the higher requirement of jumping−landing maneuvers when playing football. In cutting maneuvers, footballers showed less hip and knee flexion than futsal players, whereas the knee valgus angle in cutting maneuvers was lower in futsal players. More information on the injury mechanisms of landing and cutting in football and futsal are needed to improve the design of injury prevention programs

Differential Methylation of the HPV 16 Upstream Regulatory Region during Epithelial Differentiation and Neoplastic Transformation

High risk human papillomaviruses are squamous epitheliotropic viruses that may cause cervical and other cancers. HPV replication depends on squamous epithelial differentiation. Transformation of HPV-infected cells goes along with substantial alteration of the viral gene expression profile and preferentially occurs at transformation zones usually at the uterine cervix. Methylation of the viral genome may affect regulatory features that control transcription and replication of the viral genome. Therefore, we analyzed the methylation pattern of the HPV16 upstream regulatory region (URR) during squamous epithelial differentiation and neoplastic transformation and analyzed how shifts in the HPV URR methylome may affect viral gene expression and replication. HPV 16 positive biopsy sections encompassing all stages of an HPV infection (latent, permissive and transforming) were micro-dissected and DNA was isolated from cell fractions representing the basal, intermediate, and superficial cell layers, each, as well as from transformed p16INK4a-positive cells. We observed fundamental changes in the methylation profile of transcription factor binding sites in the HPV16 upstream regulatory region linked to the squamous epithelial differentiation stage. Squamous epithelial transformation indicated by p16INK4a overexpression was associated with methylation of the distal E2 binding site 1 leading to hyper-activation of the HPV 16 URR. Adjacent normal but HPV 16-infected epithelial areas retained hyper-methylated HPV DNA suggesting that these viral genomes were inactivated. These data suggest that distinct shifts of the HPV 16 methylome are linked to differentiation dependent transcription and replication control and may trigger neoplastic transformation