IN VIVO EVALUATION OF BUDESONIDE MICROSPHERES FOR COLON SPECIFIC DRUG DELIVERY

Objective: The aim of the present study was to evaluate the in vivo behaviour of budesonide microspheres for colon targeted delivery.Methods: In vivo therapeutic effect was evaluated using trinitrobenzene sulfonic acid (TNBS) induced colitis in rats. The therapeutic effect was assessed by determining the damage score, clinical activity score, colon/body weight(C/B) ratio and myeloperoxidase (MPO) activity measurement. The data were compared with standard drug 5-aminosalicylic acid (5-ASA). The targeting efficiency of the formulation was assessed by X-ray studies on the rabbit.Results: The study showed that oral administration of budesonide microspheres exerted an affirmative impact on the colonic ulcer healing by decreasing the area of ulceration, reducing the mass of colon by improving the symptoms of colitis. MPO activity decreased significantly after oral administration of microspheres. Histopathological studies carried out also confirmed the result. The X-ray studies revealed that the formulations were able to target the colon.Conclusion: The in vivo study confirmed the ability of budesonide microspheres in targeting the colonic region.Keywords: Budesonide, Colitis, Targeted delivery, Myeloperoxidase, Ulceration, Clinical activity scor

The value of kinetic glomerular filtration rate estimation on medication dosing in acute kidney injury.

BackgroundIn acute kidney injury (AKI), medication dosing based on Cockcroft-Gault creatinine clearance (CrCl) or Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) estimated glomerular filtration rates (eGFR) are not valid when serum creatinine (SCr) is not in steady state. The aim of this study was to determine the impact of a kinetic estimating equation that incorporates fluctuations in SCrs on drug dosing in critically ill patients.MethodsWe used data from participants enrolled in the NIH Acute Respiratory Distress Syndrome Network Fluid and Catheters Treatment Trial to simulate drug dosing category changes with the application of the kinetic estimating equation developed by Chen. We evaluated whether kinetic estimation of renal function would change medication dosing categories (≥60, 30-59, 15-29, and <15mL/min) compared with the use of CrCl or CKD-EPI eGFR.ResultsThe use of kinetic CrCl and CKD-EPI eGFR resulted in a large enough change in estimated renal function to require medication dosing recategorization in 19.3% [95 CI 16.8%-21.9%] and 23.4% [95% CI 20.7%-26.1%] of participants, respectively. As expected, recategorization occurred more frequently in those with AKI. When we examined individual days for those with AKI, dosing discordance was observed in 8.5% of total days using the CG CrCl and 10.2% of total days using the CKD-EPI equation compared with the kinetic counterparts.ConclusionIn a critically ill population, use of kinetic estimates of renal function impacted medication dosing in a substantial proportion of AKI participants. Use of kinetic estimates in clinical practice should lower the incidence of medication toxicity as well as avoid subtherapeutic dosing during renal recovery

Demographics and presenting clinical features of childhood systemic lupus erythematosus

Objectives: To review the presentation and characteristics of children with systemic lupus erythematosus (SLE).Methods: The records of children with sufficient American College of Rheumatology (ACR) criteria for SLE treated by the renal units of the Johannesburg and Chris Hani Baragwanath hospitals, and the arthritis clinic of the Johannesburg Hospital between January 1974 and March 2000 were reviewed. The clinical presentation, age distribution and race were examined.Results: A total of 36 children met the criteria. There were 26 girls and 10 boys, with a mean age of 11.5 and 10.2 years respectively. The male-to-female ratio was 1:2.6 overall, with a ratio of 1:1.2 under 10 years and 1:4 over 10 years. There were 15 white, 2 Indian and 5 coloured patients. The 14 black patients all presented after 1986. Rashes were found to be the commonest clinical feature present at the time of diagnosis, followed by polyarthritis and renal pathology. Constitutional symptoms were common, as were generalised lymphadenopathy and hepatosplenomegaly, while neurological, pulmonary and cardiac signs and symptoms were less common. Renal disease was present in 58% of patients on presentation.Conclusion: There is a diverse array of presenting features in childhood SLE. There has been increased recognition of the disease in young black South Africans since 1986.Journal of Endocrinology, Metabolism and Diabetes of South Africa Vol. 10(2) 2005: 64-6

Recital Comparison of Bilingual Language Using Various Filters for Offline Handwritten Character

Optical Character Recognition (OCR) of multilingual document containing Offline Handwritten Character (OHC) in regional languages of India, it is necessary to identify different script forms before running an individual OCR of the scripts. In this paper, novel approaches for offline character recognition are written in south Indian languages such as Tamil and Kannada. Preprocessing is one of the most important phases in OCR development. It directly affects the efficiency of any OCR. In this process an extracting of basic constituent symbols of the script. Different methodologies which are growing rapidly in the area of character recognition is South Indian Languages. In this paper, it is mainly focused on the existing methodology used in different stages of OCR to recognize offline handwritten character of bilingual regional languages of South India such as Tamil and Kannada are reviewed, summarized and documented

Hormonal effects of drosperinone and ethinyl estradiol based combined oral contraceptives on polycystic ovarian syndrome

Background: Polycystic ovarian syndrome (PCOS) is a common endocrine disorder treated with combined oral contraceptives (COCs). The androgenic potential of any type of COC is in part determined by the progestin present. A newer form of progestin, drospirenone, has been recently introduced, and is available in combination with ethinyl estradiol.Methods: The study was conducted on 60 patients fulfilling inclusion criteria, in the department of obstetrics and gynecology, Terna Medical College and Hospital. Patients were diagnosed as PCOS according to Rotterdam criteria. After initial evaluation, blood samples were sent for levels of serum luteinizing hormone (LH), follicle stimulating hormone (FSH), dehydroepiandrosterone-sulphate (DHEA-S), free testosterone, sex hormone binding globulin (SHBG). Each patient was advised to take a combination of EE (30 mcg) + drosperinone (3 mg), 1 tablet daily from the second day of her menstruation for 21 days then a 7 days gap and again for 21 days and so on cyclically for 3 cycles, then to repeat the tests as done at the beginning.  Hirsutism was assessed clinically using the Ferriman-Gallwey scale at initial visit and three months later.Results: We found a significant improvement in the Serum LH, FSH levels and a significant fall in free testosterone levels accompanied by a rise in SHBG levels. There is also overall improvement in the hirsutism scores.Conclusions: Drosperinone based COCs are a good and effective means of treatment of PCOS. Treatment duration of three months was found to be effective in our study

Prune belly syndrome: a South African perspective

Background: Prune belly syndrome (PBS) is a rare congenital disorder with a triad of signs: absent abdominal wall musculature, urinary tract malformations, and cryptorchidism. The aims of this study were to describe the epidemiology, renal function, management and outcome of patients with PBS at the Chris Hani Baragwanath Academic Hospital, Johannesburg, South Africa.Methods: A retrospective record review was conducted of patients with PBS referred to the paediatric nephrology unit from 1984 to 2014.Results: A total of 44 patient records were analysed. Most of the patients presented as neonates (n = 35, 80%). The accompanying congenital malformations included urological problems (21, 48%), orthopaedic abnormalities (5, 11%), congenital heart disease (3, 7%) and gastrointestinal abnormalities (2, 4%). The medical management included the use of prophylactic antibiotics and intermittent bladder catheterisation. Surgical management included abdominoplasty with orchidopexy and circumcision. At the last clinic visit, severe reductions in renal function were noted in 11 (58%) of the patients under the age of 2 years, whereas normal renal function was present in 14 (56%) of the patients over 2 years of age. The default rate was 57%; 6 (14%) patients were transferred to an adult clinic and 2 (4%) patients died.Conclusions: The patient profiles and the management recorded are comparable with other reported series. There are differences in the frequencies of congenital malformations and the rates of surgical intervention. The renal outcome for children older than two years was favourable compared to younger children. Progression to end-stage renal disease was observed infrequently, but this may be underestimated in view of the high default rate

Effect of rice husk biochar, carpet waste, farm yard manure and plant growth promoting rhizobium on the growth and yield of rice (Oryza sativa)

The present investigation was aimed for improving growth and yield of crop using waste products of differ-ent activities and also useful in ecological stability of soil environment. This objective is not only an economic option for poor farmer but also an effective strategy for increasing yield. The experiment was conducted in the organic farm-ing plot of the Institute of Agricultural Sciences, BHU, Varanasi during kharif season of rice crop in 2014. The field experiment was laid out in randomized block design (RBD) with 10 treatments and three replications. Application of graded level of biochar, carpet waste farm yard manure (FYM) and plant growth promoting rhizobium (PGPR) was found to significantly enhance the grain and straw yield of rice by 57.70% and 56.08% over control, respectively

Leading Through Partnership: An Examination of Longitudinal Trends in a School-University Partnership

The purpose of this manuscript is to detail pre- and post- changes that occurred after a principal, Dr. D, was moved from one middle school to another. Dr. D brought an established partnership with him to his new school including a teacher residency program, UTeach replication program, and an on-site course. The results of ANOVAs are presented regarding the school climate according to student and teacher perspectives. Significant results were found on a number of variables including student belonging, bullying, and teacher autonomy. This manuscript addresses the National Association for Professional Development Schools Essential #5: Research and Results