Piperacillin-Tazobactam (TZP) Resistance in Escherichia coli Due to Hyperproduction of TEM-1 β-Lactamase Mediated by the Promoter Pa/Pb

TEM-1, mediated by plasmid and transposon, is the most commonly encountered β-lactamase in Gram-negative bacteria. Four different promoters upstream of blaTEM-related genes have been identified: the weak P3 promoter, and the strong promoters Pa/Pb, P4, and P5. In this study, we investigated the genetic basis of a clinical strain of Escherichia coli (RJ904), which was found to be resistant to BLBLIs (β-lactam/β-lactamase inhibitors), including amoxicillin-clavulanate, ticarcillin-clavulanate (TCC), and piperacillin-tazobactam (TZP) but sensitive to third-generation cephalosporins. The conjugation test and S1-nuclease pulsed-field gel electrophoresis (S1-PFGE) demonstrated that transfer of this resistance was mediated by a ca. 100 kb plasmid. The transformant with TZP resistance was screened out with the shortgun cloning. Sequence analysis revealed that the recombinant plasmid contained a blaTEM-1b gene with the strong promoter Pa/Pb. Different plasmids were cloned based on the clone vector pACYC184 with the insertion of the blaTEM-1b gene with promoters Pa/Pb or P3. Susceptibility to TZP was determined by the E-test, agar dilution, and broth microdilution. The level of blaTEM-1b-specific transcription was determined by quantitative real-time PCR. Substitution of Pa/Pb for P3 resulted in a 128-fold decline of the MIC value of TZP, from >1024 mg/L to 8 mg/L, and a significantly lower blaTEM-1b expression level. Hyperproduction of TEM-1 β-lactamase mediated by the promoter Pa/Pb was responsible for high resistance to TZP in E. coli. Our data show possible risks of resistance development in association with the clinical use of TZP. The blaTEM promoter modifications should be considered for whole genome whole-genome sequencing-inferred bacterial antimicrobial susceptibility testing

Giant Enhancement of Magnonic Frequency Combs by Exceptional Points

With their incomparable time-frequency accuracy, frequency combs have significantly advanced precision spectroscopy, ultra-sensitive detection, and atomic clocks. Traditional methods to create photonic, phononic, and magnonic frequency combs hinge on material nonlinearities which are often weak, necessitating high power densities to surpass their initiation thresholds, which subsequently limits their applications. Here, we introduce a novel nonlinear process to efficiently generate magnonic frequency combs (MFCs) by exploiting exceptional points (EPs) in a coupled system comprising a pump-induced magnon mode and a Kittel mode. Even without any cavity, our method greatly improves the efficiency of nonlinear frequency conversion and achieves optimal MFCs at low pump power. Additionally, our novel nonlinear process enables excellent tunability of EPs using the polarization and power of the pump, simplifying MFC generation and manipulation. Our work establishes a synergistic relationship between non-Hermitian physics and MFCs, which is advantages for coherent/quantum information processing and ultra-sensitive detection.Comment: 7 pages, 4 figure

High-Qf value and temperature stable Zn2+-Mn4+ cooperated modified cordierite-based microwave and millimeter-wave dielectric ceramics

Cordierite-based dielectric ceramics with a lower dielectric constant would have significant application potential as dielectric resonator and filter materials for future ultra-low-latency 5G/6G millimeter-wave and terahertz communication. In this article, the phase structure, microstructure and microwave dielectric properties of Mg2Al4–2x(Mn0.5Zn0.5)2xSi5O18 (0 ≤ x ≤ 0.3) ceramics are studied by crystal structure refinement, scanning electron microscope (SEM), the theory of complex chemical bonds and infrared reflectance spectrum. Meanwhile, complex double-ions coordinated substitution and two-phase complex methods were used to improve its Q×f value and adjust its temperature coefficient. The Q×f values of Mg2Al4–2x(Mn0.5Zn0.5)2xSi5O18 single-phase ceramics are increased from 45,000 [email protected] GHz (x = 0) to 150,500 [email protected] GHz (x = 0.15) by replacing Al3+ with Zn2+-Mn4+. The positive frequency temperature coefficient additive TiO2 is used to prepare the temperature stable Mg2Al3.7(Mn0.5Zn0.5)0.3Si5O18-ywt%TiO2 composite ceramic. The composite ceramic of Mg2Al3.7(Mn0.5Zn0.5)0.3Si5O18-ywt%TiO2 (8.7 wt% ≤ y ≤ 10.6 wt%) presents the near-zero frequency temperature coefficient at 1225 °C sintering temperature: εr = 5.68, Q×f = 58,040 GHz, τf = −3.1 ppm/°C (y = 8.7 wt%) and εr = 5.82, Q×f = 47,020 GHz, τf = +2.4 ppm/°C (y = 10.6 wt%). These findings demonstrate promising application prospects for 5 G and future microwave and millimeter-wave wireless communication technologies

Salt-Free Dyeing of Cotton Fabric Using 3-Chloro-2-Hydroxypropyltrimethyl Ammonium Chloride by Pad-Irradiate-Pad-Steam Process, and Prediction of Its K/S Value by LS-SVM

Based on conventional pad-dry-pad-steam (PDPS) process, 3-chloro-2-hydroxypropyltrimethylammonium chloride was used as an etherifying agent to cationize cotton by the pad-irradiate process by microwave and then the cationized fabric was salt-free dyed by the pad-steam process. The results showed that the dyeability of the cationized fabric with salt-free reactive dye was nearly equivalent with conventional PDPS process. This study also developed a model to predict K/S value of dyed cotton fabric based on least squares support vector machine (LS-SVM). The simulation results based on actual run data demonstrated that the model using LS-SVM has high prediction accuracy and can be employed to predict the K/S value of dyed fabric using pad-irradiate-pad-steam process

AgCu NP Formation by the Ag NP Catalysis of Cu Ions at Room Temperature and Their Antibacterial Efficacy: A Kinetic Study

Although a facile route to prepare AgCu nanoalloys (NAs) with enhanced antibacterial efficacy using Ag NP catalysis of Cu ions at elevated temperatures was previously developed, its detailed reaction process is still unclear due to the fast reaction process at higher temperatures. This work found that AgCu NAs can also be synthesized by the same process but at room temperature. AgCu NAs formation kinetics have been studied using UV–Visible spectra and Transmission Electron Microscopy (TEM), where formation includes Cu(2+) deposition onto the Ag NP surface and Ag(+) release, reduction, and agglomeration to form new Ag NPs; this is followed by a redistribution of the NA components and coalescence to form larger AgCu NPs. It is found that SPR absorption is linear with time early in the reaction, as expected for both pseudo-first-order (PFO) and pseudo-second-order (PSO) kinetics; neither model is followed subsequently due to contributions from newly formed Ag NPs and AgCu NAs. The antibacterial efficacy of the AgCu NAs thus formed was estimated, with a continuous increase over the whole alloying process, demonstrating the correlation of antibacterial efficacy with the extent of AgCu NA formation and Ag(+) release

Graft Diameter Should Reflect the Size of the Native Anterior Cruciate Ligament (ACL) to Improve the Outcome of ACL Reconstruction: A Finite Element Analysis

The size of the anterior cruciate ligament (ACL) often varies between individuals, but such variation is not typically considered during ACL reconstruction (ACLR). This study aimed to explore how the size of the ACL affects the selection of a suitable graft diameter. A finite element model of a human knee was implanted with intact ACLs of different dimensions (0.95, 1 and 1.05 times the size of the original ACL) and with grafts of different diameters, to simulate ACLR (diameter 7.5–12 mm in 0.5 mm increments). The knee models were flexed to 30° and loaded with an anterior tibial load of 103 N, internal tibial moment of 7.5 Nm, and valgus tibial moment of 6.9 Nm. Knee kinematics (anterior tibial translation (ATT), internal tibial rotation (ITR) and valgus tibial rotation (VTR)) and ligament forces were recorded and compared among the different groups. The results showed that, compared with the intact knee, a graft diameter of 7.5 mm was found to increase the ATT and VTR, but reduce the graft force. Increasing the graft diameter reduced knee laxity and increased the graft force. A 10% increase in the size of the ACL corresponded to a 3 mm larger graft diameter required to restore knee stability and graft force after ACLR. It was concluded that the graft diameter should be selected according to the dimensions of the native ACL, for better restoration of knee functionality. This study may help to improve the clinical treatment of ACL ruptures

Cooperative Search Optimization of an Unknown Dynamic Target Based on the Modified TPM

Aiming to address the problem of unknown dynamic target trajectory prediction and search path optimization in unmanned aerial vehicle (UAV) swarm path planning, this paper proposes a target search algorithm based on a modified target probability map (TPM). First, using the TPM, the proposed algorithm generates a high-probability distribution region of a target with directionality to fit the target trajectory and realizes the trajectory prediction of an unknown dynamic target. Then, the distributed ant colony (ACO) algorithm and the artificial potential field (APF) algorithm are combined to generate and optimize the UAV swarm search result and return path with the goal of maximizing task execution efficiency. Finally, the Monte Carlo simulation method is used to analyze the effectiveness of the proposed algorithm, and the results are evaluated from five aspects, including the number of targets captured. The simulation results show that under the condition of an unknown dynamic target trajectory, the average target captured rate and average unknown region search rate of the MTPM method were higher than that of the traditional TPM method, and the performance was improved by 14.6% and 10.7%, respectively

Effect of Particle Diameter on Primary Breakup of High-Pressure Diesel Spray Atomization: A Study Based on Numerical Simulations Using the Eulerian–Lagrangian Model

The coupling of Eulerian and Lagrangian methods in the Eulerian–Lagrangian Spray Atomization (ELSA) approach is critical. This study proposes an equation for the primary breakup particle diameter D of a diesel fuel spray and adopts it as a key transition criterion for coupling. A three-dimensional diesel spray is modeled by the large-eddy simulation (LES) approach. This improved ELSA simulation was conducted using various transition criteria for particle diameter Dcr. The results show that fuel spray experiences two stages: stage I, when a liquid column appears without a dispersed phase, and stage II, when primary breakup occurs with many discrete particles. Although Dcr has little influence on the macro-spray characteristics, such as top penetration distance S and spray cone angle θ, it has significant effects on discrete particles, such as their number, average diameter, distribution and location, and spray cone area. Dcr should be determined on the basis of actual operating conditions

Investigation on Primary Breakup of High-Pressure Diesel Spray Atomization by Method of Automatic Identifying Droplet Feature Based on Eulerian–Lagrangian Model

To investigate primary breakup close to an injector, this paper presents both experimental and numerical research on high-pressure common-rail diesel injection. We propose a new method named SD-ELSA model to realize automatically identifying droplet features for high-pressure diesel spray based on the classic ELSA (Eulerian Lagrangian Spray Atomization) model; this method is suitable for varied injection operation conditions. The SD-ELSA first identifies the liquid bulk due to breakup of the continuous phase in near field, and then converts the Eulerian liquid bulk into Lagrangian particles to complete the calculation of the total spray atomization. The SD-ELSA model adopts two key criteria, i.e., the sphericity (S) and the particle diameter (D); the qualified liquid mass is transformed into Lagrangian particle, realizing the coupling of the Eulerian–Lagrangian model. The SD-ELSA model illustrates the total diesel spray atomization process from the breakup liquid column to the droplets