Standardized metadata collection to reinforce collaboration in Collaborative Research Centers

We present our approach for research (meta)data exchange and interconnection of scientists in medium-scale academic projects such as medical collaborative research centers (CRCs). Our webbased tool, fredato, connects established services, which we configure to a custom-tailored software bundle to match the needs of researchers. To improve collaboration, we implemented a metadata acquisition component and search function to complement the existing data management and sharing. More specifically, we enhance three points: 1. Relevant projects need to be findable to re-use data or results, avoid redundant work and improve communication among crc members. 2. The scientists also process sensitive human data for which a privacy-protected, secure exchange is critical. 3. A self-explanatory user interface is required for increased user acceptance. The main feature we present is the handling of metadata in our web application without overwhelming users through extensive and generic forms. We use flexible JSON schemas to precisely target scientists' needs of documentation and enrich them with only ontology components relevant to their use cases. The schemas are stored distributedly as datasets themselves, and automatically converted to modern web forms. Besides custom, domain-specific forms we use this editor for the addition of common metadata schemas (e.g. DataCite). We use the continuous integration capabilities of a connected Gitlab to run data-driven scripts. This includes indexing of all metadata, which makes them searchable in a structured way. Users are provided with contact information for matches and can ask to share data and results. Because project owners decide with whom to share and because datasets and metadata are bundled together, the complete dataset is always accessible (e.g. for publication at a third party repository) and full control retained by data owners. By adding this component to our tool, we provide simple, secure and searchable means for improving collaboration in the context of CRCs

Induction of Cell Stress in Neurons from Transgenic Mice Expressing Yellow Fluorescent Protein: Implications for Neurodegeneration Research

Peer reviewedPublisher PD

Dengue virus envelope protein domain I/II hinge determines long-lived serotype-specific dengue immunity

Dengue virus is the most important arthropod-borne viral disease of humans worldwide, with an estimated 390 million acute infections annually. The best means to control this global health threat is a vaccine, but dengue vaccine development has progressed slowly, partly because the antigenic targets required to stimulate long-term immunity are not well-defined. Here, we show a specific region on the viral surface (the envelope domain I/II hinge) that is the target of protective antibodies after primary human infections. These results are critically important for dengue vaccine design, because we hypothesize that a successful dengue vaccine will stimulate antibodies that target this region. More broadly, this study establishes a template for similar approaches for improving vaccines for influenza, HIV, hepatitis C virus, and other clinically important viral pathogens

Windborne long-distance migration of malaria mosquitoes in the Sahel

Over the past two decades efforts to control malaria have halved the number of cases globally, yet burdens remain high in much of Africa and the elimination of malaria has not been achieved even in areas where extreme reductions have been sustained, such as South Africa1,2. Studies seeking to understand the paradoxical persistence of malaria in areas in which surface water is absent for 3–8 months of the year have suggested that some species of Anopheles mosquito use long-distance migration3. Here we confirm this hypothesis through aerial sampling of mosquitoes at 40–290 m above ground level and provide—to our knowledge—the first evidence of windborne migration of African malaria vectors, and consequently of the pathogens that they transmit. Ten species, including the primary malaria vector Anopheles coluzzii, were identified among 235 anopheline mosquitoes that were captured during 617 nocturnal aerial collections in the Sahel of Mali. Notably, females accounted for more than 80% of all of the mosquitoes that we collected. Of these, 90% had taken a blood meal before their migration, which implies that pathogens are probably transported over long distances by migrating females. The likelihood of capturing Anopheles species increased with altitude (the height of the sampling panel above ground level) and during the wet seasons, but variation between years and localities was minimal. Simulated trajectories of mosquito flights indicated that there would be mean nightly displacements of up to 300 km for 9-h flight durations. Annually, the estimated numbers of mosquitoes at altitude that cross a 100-km line perpendicular to the prevailing wind direction included 81,000 Anopheles gambiae sensu stricto, 6 million A. coluzzii and 44 million Anopheles squamosus. These results provide compelling evidence that millions of malaria vectors that have previously fed on blood frequently migrate over hundreds of kilometres, and thus almost certainly spread malaria over these distances. The successful elimination of malaria may therefore depend on whether the sources of migrant vectors can be identified and controlled

Inhibition of Nipah Virus Infection In Vivo: Targeting an Early Stage of Paramyxovirus Fusion Activation during Viral Entry

In the paramyxovirus cell entry process, receptor binding triggers conformational changes in the fusion protein (F) leading to viral and cellular membrane fusion. Peptides derived from C-terminal heptad repeat (HRC) regions in F have been shown to inhibit fusion by preventing formation of the fusogenic six-helix bundle. We recently showed that the addition of a cholesterol group to HRC peptides active against Nipah virus targets these peptides to the membrane where fusion occurs, dramatically increasing their antiviral effect. In this work, we report that unlike the untagged HRC peptides, which bind to the postulated extended intermediate state bridging the viral and cell membranes, the cholesterol tagged HRC-derived peptides interact with F before the fusion peptide inserts into the target cell membrane, thus capturing an earlier stage in the F-activation process. Furthermore, we show that cholesterol tagging renders these peptides active in vivo: the cholesterol-tagged peptides cross the blood brain barrier, and effectively prevent and treat in an established animal model what would otherwise be fatal Nipah virus encephalitis. The in vivo efficacy of cholesterol-tagged peptides, and in particular their ability to penetrate the CNS, suggests that they are promising candidates for the prevention or therapy of infection by Nipah and other lethal paramyxoviruses

The EnMAP imaging spectroscopy mission towards operations

EnMAP (Environmental Mapping and Analysis Program) is a high-resolution imaging spectroscopy remote sensing mission that was successfully launched on April 1st, 2022. Equipped with a prism-based dual-spectrometer, EnMAP performs observations in the spectral range between 418.2 nm and 2445.5 nm with 224 bands and a high radiometric and spectral accuracy and stability. EnMAP products, with a ground instantaneous field-of-view of 30 m x 30 m at a swath width of 30 km, allow for the qualitative and quantitative analysis of surface variables from frequently and consistently acquired observations on a global scale. This article presents the EnMAP mission and details the activities and results of the Launch and Early Orbit and Commissioning Phases until November 1st, 2022. The mission capabilities and expected performances for the operational Routine Phase are provided for existing and future EnMAP users

Gender Stereotypes and Youth Violence

This presentation was given during the Southern Criminal Justice Association Annual Meeting

Does Breaking Gender Stereotypes Contribute to Victimization at School?

Victimization is a serious problem facing youth in American public schools. Prior research demonstrates that victimization is stratified by sex/gender; however, few studies consider factors that may moderate this relationship. This research investigates if victimization occurs when students break sex/gender stereotypes at school among female and male youth. The broad research question for this study is – are breaking sex/gender stereotypes regarding academic activities, math, and sports associated with victimization for female and male students at school? To address this research question, this study employs nationally representative data from the Education Longitudinal Study (ELS) of 2002 to investigate if breaking sex/gender stereotypes contributes to the likelihood of victimization for female and male students at school. The study finds that females who have favorable attitudes toward math and participate in math-related activities are less likely to be victimized, while students of both sexes who participate in female dominated sports (i.e. cheerleading and softball) have a higher risk of victimization, as do females who participate in male dominated sports (i.e. football and baseball). The implications for future research and policy implementation are discussed

TRIM19/PML Restricts HIV Infection in a Cell Type-Dependent Manner

The promyelocytic leukemia protein (PML) is the main structural component of the nuclear matrix structures termed nuclear domain 10 (ND10) or PML nuclear bodies (PML-NBs). PML and ND10 structures have been shown to mediate an intrinsic immune response against a variety of different viruses. Their role during retroviral replication, however, is still controversially discussed. In this study, we analyzed the role of PML and the ND10 components Daxx and Sp100 during retroviral replication in different cell types. Using cell lines exhibiting a shRNA-mediated knockdown, we found that PML, but not Daxx or Sp100, inhibits HIV and other retroviruses in a cell type-dependent manner. The PML-mediated block to retroviral infection was active in primary human fibroblasts and murine embryonic fibroblasts but absent from T cells and myeloid cell lines. Quantitative PCR analysis of HIV cDNA in infected cells revealed that PML restricts infection at the level of reverse transcription. Our findings shed light on the controversial role of PML during retroviral infection and show that PML contributes to the intrinsic restriction of retroviral infections in a cell type-dependent manner