Tissue eosinophils as a histopathological marker in oral premalignant and malignant lesions: a histochemical study

Introduction: Although recent studies show the association of eosinophils with different tumors including oral malignancies, the role of eosinophils in premalignant conditions of the oral cavity is not known precisely. Therefore, this study aimed to determine the number of eosinophils in premalignant and malignant conditions of the oral cavity and its role in progression to malignancy. Materials &Methods: This cross-sectional study was done on 50 samples with oral squamous cell carcinoma (OSCC), 18 with verrucous carcinoma, 13 with erosive lichen planus, and 10 with leukoplakia with mild dysplasia after confirmation of histopathological diagnosis. The samples were stained with Sirius Red. The total numbers of eosinophils in 10 areas were counted at a magnification of 400x (eosinophil/10HPF). Data are analyzed with Kruskal-Wallis test, Mann-Whitney test, and Spearman’s correlation test. Results: The mean number of eosinophils in OSCC, verrucous carcinoma, erosive lichen planus, and leukoplakia with mild dysplasia was 90.26, 59.72, 11.08 and 0.0 eosinophil/10HPF respectively. The mean number of eosinophils was significantly higher in malignant (OSCC and verrucous carcinoma) than premalignant (erosive lichen planus and leukoplakia with mild dysplasia) lesions of the oral cavity (P=0.0001). No significant correlation was found between the number of eosinophils with histopathologic grade, gender, and age in OSCC samples. Conclusion: The greater number of eosinophils is seen in malignant than premalignant lesions of the oral cavity. It is recommended to consider the high number of eosinophils in premalignant lesions as an indicator for a more potential of preliminary oral lesions to undergo malignant transformation

Epidemiological aspects of head and neck cancers in a population of south east region of Iran

Introduction: The geographical distribution of head and neck cancer shows considerable variation and it is site dependent. The review of the literature shows few data concerning the head and neck cancer patterns in Iran. The purpose of this study was to represent epidemiological aspects of head and neck cancers in south east of Iran. Materials &Methods: In this retrospective study, from a total of 924 diagnosed patients with cancer in the pathology department of dentistry school archives and two main hospitals in south east of Iran during 2006-2012, 287 cases of head and neck cancer were diagnosed histopathologically. Demographic data of patients such as age, gender, location and type of cancer (carcinoma, sarcoma, melanoma, metastatic, or primary) were obtained from patients’ records. Data were analyzed by SPSS statistical software (V.21) using the chi-square test. Results: Squamous cell carcinoma (39.7%) and basal cell carcinoma (26.8%) were the most common malignancies in the head and neck region. The mean age of the patients was 56.1±12.8 years and the relationship between age groups and head and neck cancers was not statistically significant (P=0.085). Male to female ratio was 0.9 to 1. The relationship between gender and types of head and neck cancers was not statistically significant. (P=0.114) Conclusion: In this study, prevalence of head and neck cancer in south east of Iran (Zahedan) was relatively high. However, comparison between findings of our study with other studies shows a relation consistency

Epidemiological aspects of head and neck cancers in a population of south east region of Iran

Introduction: The geographical distribution of head and neck cancer shows considerable variation and it is site dependent. The review of the literature shows few data concerning the head and neck cancer patterns in Iran. The purpose of this study was to represent epidemiological aspects of head and neck cancers in south east of Iran. Materials &Methods: In this retrospective study, from a total of 924 diagnosed patients with cancer in the pathology department of dentistry school archives and two main hospitals in south east of Iran during 2006-2012, 287 cases of head and neck cancer were diagnosed histopathologically . Demographic data of patients such as age, gender, location and type of cancer (carcinoma, sarcoma, melanoma, metastatic, or primary) were obtained from patients’ records. Data were analyzed by SPSS statistical software (V.21) using the chi-square test. Results: Squamous cell carcinoma (39.7%) and basal cell carcinoma (26.8%) were the most common malignancies in the head and neck region. The mean age of the patients was 56.1±12.8 years and the relationship between age groups and head and neck cancers was not statistically significant (P=0.085). Male to female ratio was 0.9 to 1. The relationship between gender and types of head and neck cancers was not statistically significant.(P=0.114) Conclusion: In this study, prevalence of head and neck cancer in south east of Iran (Zahedan) was relatively high. However, comparison between findings of our study with other studies shows a relation consistency

Reactive Hyperplastic Lesions of the Oral Cavity

Introduction: Peripheral reactive lesions of soft tissue are common oral lesions that dentists face during routine examinations. Diagnosis and development of a treatment plan is difficult if dentists are not aware of the prevalence and clinical symptoms of these lesions. The frequency of these lesions differs across various populations. The aim of this study was to determine the frequency and distribution of oral reactive lesions over a period of 7 years (2006–2012).   Materials and Methods: In this retrospective study, available records from the archives of the Department of Pathology, Dental School and the two main hospitals in southeast of Iran (Zahedan) over a period of 7 years (2006–2012) were reviewed. Information relating to the type of reactive lesion, age, gender and location was extracted and recorded on data forms. Data were analyzed using SPSS statistical software (V.18) using the chi-square and Fisher’s exact test.   Results: Of 451 oral lesions, 91 cases (20.2%) were reactive hyperplastic lesions. The most common lesions were pyogenic granuloma and irritation fibroma, respectively. These lesions were more frequent in women (60%) than men (40%). The most common locations of involvement were the gingiva and alveolar mucosa of the mandible, and lesions were more common in the 21–40-year age group. The relationship between age group and reactive lesions was statistically significant (P=0.01).   Conclusion:  The major findings in this study are broadly similar to the results of previous studies, with differences observed in some cases. However, knowledge of the frequency and distribution of these lesions is beneficial when establishing a diagnosis and treatment plan in clinical practice

Effect of CDH1 and CDH2 genes polymorphisms in oral squamous cell carcinoma susceptibility in a sample of Iranian population: A case‐control study

Abstract Background and Aims Oral squamous cell carcinoma (OSCC) is a global malignant epithelial neoplasm affecting the oral cavity. Cadherins, as an adhesion molecule, are involved in cell−cell interaction. We aim to study the effect of two cadherin polymorphisms on OSCC risk in southeast of Iran. Methods In this case‐control study, 94 individuals (47 OSCC cases and 47 controls), that referred to the Department of Oral Pathology, Faculty of Dentistry, Zahedan University of Medical Sciences, Iran were included. Cadherin single nucleotide polymorphisms CDH1 (rs16260) and CDH2 (rs11564299) were genotyped by the tetra‐Amplification Refractory Mutation System—PCR technique. Results N‐cadherin genotyping showed that the AA, AG, and AG + GG were presented 78.7%, 17%, 21.3% versus 66%, 29.7%, 34% in the cases and the control group, respectively. AG genotype was more common in control than case (OR = 0.47, 95% CI: 0.17−1.29, p = 0.14). G allele was more prevalent in control (19.1%) than the case group (12.8%) (OR = 0.61, 95% CI: 0.27−1.36, p = 0.23). In E‐cadherin, AC, AA, and AC + AA genotypes frequency were 17%, 12.8%, and 29.8% in case versus 8.5%, 8.5%, and 17% in the control group. Allele A was more common in the case than the control group (OR = 1.84, 95% CI: 0.84−4.03, p = 0.12). Also, AA and CC, the codominant genotypes were common in CDH2 and CDH1 respectively in all histopathological grades, and no statically significant association was observed between OSCC different histopathological grades and cadherin genotypes (p = 0.39 in N‐cadherin, p = 0.74 in E‐cadherin). Conclusion Our results showed a lack of association between CDH1 and CDH2 gene polymorphisms with OSCC risk in a population of Southeastern of Iran

Compatibility Rate of Clinical and Histopathological Diagnosis of Oral Lesions Compatibility Rate of Clinical and Histopathologic Diagnosis of Oral Lesions in Zahedan Dental School during 1999-2015

Abstract Introduction: Different oral lesions have clinical characteristics which in some cases are similar. Therefore, in these cases histopathological examination for correct diagnosis is necessary. The aim of this study was to evaluate the compatibility rate of clinical and histopathological diagnosis of oral lesions in Zahedan School of dentistry. Methods: In this retrospective study, determination of the compatibility of clinical and histopathological diagnosis was done using 631 available records in department of pathology, Zahedan School of dentistry, during 1999-2015. Type of the lesions (neoplastic and non-neoplastic), and demographic data including age, gender, location of lesions (intraosseous or soft tissue), and clinician's specialty was extracted from patients records and data were analyzed using SPSS (V.21) software and ChiSquare test. Results: Total compatibility rate between clinical and histopathological diagnosis was 70.1%. The most accurate clinical diagnosis was related to lichenoid lesions (100%) and leukoplakia (100%) and verrucous carcinoma had the least diagnostic compatibility (20%). There was no significant relationship between compatibility of histopathological and clinical diagnosis with age range, gender, location, and clinician's specialty. Also non-neoplastic lesions with compatible histopathological and clinical diagnoses were three times more than neoplastic lesions. (P=0.03). Conclusion: Although there was a great compatibility between clinical and histopathological diagnosis, many records had no clinical diagnosis and the inconsistency was also significant. Therefore, more attention to clinical signs and effective cooperation between the clinician an

Clinical and Histopathological Profiles of Pediatric and Adolescent Oral and Maxillofacial Biopsies in a Persian Population

Introduction The frequency of pediatric and adolescent oral and maxillofacial lesions is various in different societies. The present study aimed at investigating the frequency of oral and maxillofacial pediatric and adolescent biopsies in Zahedan (southeast Iran), and compare the results with other epidemiologic studies.Methods and Materials This retrospective study reviewed oral and maxillofacial lesions in patients with 0-18 years old referring to the treatment centers of Zahedan University of Medical Sciences during 12-years period. Patients’ demographic information including age, gender and location of the lesion were collected and statistically analyzed.ResultsIn general, among 1112 oral and maxillofacial lesions, 154 (13.9%) cases were related to children and adolescents younger than 18 years old. The average age of patients was 11.4 ± 4.9, 53.2% and 46.8% of them were boys and girls, respectively. The most frequent sites of lesions were the gingiva and lip. The most prevalent lesions included inflammatory/reactive, cystic and neoplastic lesions, respectively. Benign and malignant tumors comprised 12.3% and 4.5% of cases. Moreover, pyogenic granuloma and peripheral giant cell granuloma (PGCG) were the most frequent lesions.ConclusionThe most prevalent oral and maxillofacial lesions in patients under 18 years old were inflammatory/reactive lesions in gingiva and in the 13-18 age range. Determining the characteristics of these lesions in the children and adolescents population provides a firm groundwork for proper diagnosis and treatment

Human Herpesvirus-6 and Epstein-Barr Virus Infections at Different Histopathological Grades of Oral Squamous Cell Carcinomas

Background: The aim of this study was to determine the prevalence and viral load of Epstein-Barr virus (EBV) and Human herpesvirus-6 (HHV-6) in different histopathologic grades of oral squamous cell carcinoma (OSCC). Methods: Forty-five formalin-fixed paraffin-embedded tissue section of OSCC patients were analyzed by quantitative real-time polymerase chain reaction for detection of EBV and HHV-6. Results: The mean age of the patients was 58.6 years, 69% of whom were female, and 31% were male. Overall, the positive rate for EBV and HHV-6 were 16.7% and 27.1%, respectively; and the mean viral load EBV was 27.9 × 10 3 and 38.5 × 10 3 for HHV-6. No correlation was demonstrated between the viral load of EBV DNA (P = 0.35) and HHV-6 (P = 0.38) at the different OSCC histopathologic grades. Conclusions: These findings neither lend support to the hypothesis that EBV and HHV-6 are directly involved in OSCC nor rule out the possibility that these viruses play an indirect role in carcinogenesis in this area

A comparative study of osteopontin and MMP-2 protein expression in peripheral and central giant cell granuloma of the jaw

Introduction: Oral peripheral and central giant cell granulomas are lesions with little-known etiology and pathogenesis. Objective: The aim of this study was to compare matrix metalloproteinases-2 and osteopontin protein expression in the multinucleated giant cells and mononuclear cells of the peripheral and central giant cell granuloma lesions. Methods: In this retrospective study, the presence of matrix metalloproteinases-2 and osteopontin in 37 cases of central giant cell granuloma and 37 cases of peripheral giant cell granuloma paraffin blocks were assessed by streptavidin-biotin immunohistochemistry. Independent sample t-test, Chi-square, Mann–Whitney tests and Spearman's rank correlation coefficient were used. Results: The osteopontin was expressed in both multinucleated giant cells and mononuclear cells in all cases of peripheral and central giant cells granulomas. However, the matrix metalloproteinases-2 expression was positive in 86.5% of giant cells and it was positive in all of mononuclear cells in peripheral giant cells granuloma. In central giant cells granulomas, 91.8% of giant cells and all mononuclear cells were positive for matrix metalloproteinases-2 marker. Percentage and Intensity of staining were significantly higher in central than peripheral giant cells lesions, for both markers (p ˂ 0.05). Conclusion: This study showed that the expression of osteopontin in giant cells supports the theory of osteolcastic nature of these cells. Also, the presence of osteopontin and matrix metalloproteinases-2 in mononuclear cells may indicate the monocyte-macrophage origin of these cells, as the differentiation of the precursors of the mononuclear stromal monocyte/macrophage to osteoclasts is possibly affected by the expression of osteolytic factors. Also, may be differences in biological behaviors of these lesions are associated with the level of osteopontin and matrix metalloproteinases-2 expression. Resumo: Introdução: Os granulomas periféricos e centrais de células gigantes são lesões com etiologia e patogênese pouco conhecidas. Objetivo: O objetivo deste estudo foi comparar a expressão das proteínas metaloproteinases da matriz-2 e osteopontina nas células gigantes multinucleadas e células mononucleares no granuloma periférico e central de células gigantes. Método: Neste estudo retrospectivo, a presença de metaloproteinases da matriz-2 e osteopontina em 37 casos de granuloma central de células gigantes e 37 casos de granuloma periférico de células gigantes em blocos de parafina foram avaliadas por imunohistoquímica pela estreptavidina-Biotina. Foram utilizados teste t para amostra independente, teste de Qui-quadrado, Mann-Whitney e coeficiente de correlação de Spearman. Resultados: A osteopontina foi expressa em células gigantes multinucleadas e células mononucleares em todos os casos de granuloma periférico de células gigantes e granuloma central de células gigantes. No entanto, a expressão de metaloproteinases da matriz-2 foi positiva em 86,5% de células gigantes e foi positiva em todas as células mononucleares em granuloma periférico de células gigantes. Em granuloma central de células gigantes, 91,8% das células gigantes e todas as células mononucleares foram positivas para o marcador metaloproteinases da matriz-2. A porcentagem e intensidade de coloração em granuloma central de células gigantes foram significantemente maiores do em granuloma periférico de células gigantes para ambos os marcadores (p ˂ 0,05). Conclusão: Este estudo mostrou que a expressão de osteopontina em células gigantes apoia a teoria da natureza osteoclástica dessas células. Além disso, a presença de osteopontina e metaloproteinases da matriz-2 em células mononucleares pode indicar a origem dos monócitos-macrófagos dessas células, uma vez que a diferenciação dos precursores do monócito/macrófago estromal mononuclear em osteoclastos é possivelmente afetada pela expressão de fatores osteolíticos. Além disso, as diferenças nos comportamentos biológicos dessas lesões estão associadas ao nível de expressão de osteopontina e metaloproteinases da matriz-2. Keywords: Osteopontin, MMP-2, PGCG, CGCG, Immunohistochemistry, Palavras-chave: Osteopontina, MMP-2, PGCG, CGCG, Imunohistoquímic