Proteomic analysis of sweet algerian apricot kernels ( Prunus armeniaca L.) by combinatorial peptide ligand libraries and LC-MS/MS

An investigation on the proteome of the sweet kernel of apricot, based on equalisation with combinatorial peptide ligand libraries (CPLLs), SDS-PAGE, nLC-ESI-MS/MS, and database search, permitted identifying 175 proteins. Gene ontology analysis indicated that their main molecular functions are in nucleotide binding (20.9 %), hydrolase activities (10.6 %), kinase activities (7 %), and catalytic activity (5.6%). A protein-protein association network analysis using STRING software permitted to build an interactomic map of all detected proteins, characterised by 34 interactions. In order to forecast the potential health benefits deriving from the consumption of these proteins, the two most abundant, i.e. Prunin 1 and 2, were enzymatically digested in silico predicting 10 and 14 peptides, respectively. Searching their sequences in the database BIOPEP, it was possible to suggest a variety of bioactivities, including dipeptidyl peptidase-IV (DPP-IV) and angiotensin converting enzyme I (ACE) inhibition, glucose uptake stimulation and antioxidant properties

Quel avenir pour les oiseaux en région méditerranéenne ?

A l'occasion de la publication du n°100 de la revue Forêt Méditerranéenne, il a été demandé aux grandes institutions nationales qu'elles nous présentent leur vision des espaces forestiers méditerranéens "depuis Paris". Cet article nous donne le regard porté par les ornithologues (Ligue pour la protection des oiseaux) sur la forêt méditerranéenne

[Cp*Ru]-catalyzed selective coupling/hydrogenation

International audienc

Antibacterial Activity and Chemical Composition of Essential Oil of Athamanta sicula L. (Apiaceae) from Algeria

Essential oil extracted from fresh aerial parts of Athamanta sicula L. (syn. Tingara sicula) was analysed by gas phase chomatography coupled to mass spectrometry (GC-MS). The main constituents were: germacrene B (88.5%) and apiol (4.9%). Comparing with the tested bacteria, the growth of Escherichia coli and Klebsiella pneumoniae strains was more inhibited by the essential oil of A. sicula

Antibacterial activity and chemical composition of essential oil of Ammi visnaga L. (Apiaceae) from Constantine, Algeria.

Abstract: Essential oil extracted from fresh aerial parts of Athamanta sicula L. (syn. Tingara sicula) was analysed by gas phase chomatography coupled to mass spectrometry (GC-MS). The main constituents were: germacrene B (88.5%) and apiol (4.9%). Comparing with the tested bacteria, the growth of Escherichia coli and Klebsiella pneumoniae strains was more inhibited by the essential oil of A. sicula

A New Alkaloid and Flavonoids from the Aerial Parts of Euphorbia guyoniana

One novel alkaloid, 1,5-diphenyl-3-styryl-2-pyrazoline 1, in addition with six known flavonoids namely, kaempferol, kaempferol 3-O-glucoside, kaempferol 3-rutinoside, quercetin, quercetin 3-O-glucoside, and rutin, were isolated from the aerial parts of Euphorbia guyoniana. Their structures were established on the basis of physical and spectroscopic analysis, and by comparison with the literature data.The authors are grateful to the ANDRS and DG-RSDT at the MESRS (Ministry of Scientific Research, Algeria) for the financial support.Peer Reviewe

Components and antioxidant, anti-inflammatory, anti-ulcer and antinociceptive activities of the endemic species Stachys mialhesi de Noé

© 2011 Production and hosting by Elsevier B.V. on behalf of King Saud UniversityOne diterpenoid, horminone 1, two flavonoid glycosides, apigenin-7-O-(6″-E-p-coumaroyl)-β-d-glucopyranoside 2, isoscutellarein-7-O-(6″′-O-acetyl-β-d-allopyranosyl-(1 → 2)-β-d-glucopyranoside) 3, were isolated from n-butanolic extract of the aerial parts of Stachys mialhesi de Noé (BESM). Their structures were established on the basis of physical and spectroscopic analysis, and by comparison with the literature data. Antioxidant activity of this extract and the compound 3 was evaluated by the use of the Electron Spin Resonance method in order to visualize the inhibition of the DPPH radical. In this study, we also investigated the anti-inflammatory, anti-ulcer and antinociceptive activities of the BESM in experimental animal models at different doses. Our results showed that the BESM showed a strongest antioxidant activity. It decreased acetic acid induced writhing times; inhibited carrageenan-induced hind paw edema. All of these results suggested that the BESM possesses significant antioxidant, antinociceptive and anti-inflammatory activities.The authors are grateful to the ANDRS and DG-RSDT/MESRS for the financial support.Peer Reviewe

Secondary metabolites, evaluation of the DPPH Free-Radical Scavenging Effect by electron spin resonance and antibacterial activity of the endemic species Stachys circinata

The genus Stachys (Lamiaceae), widely known in folk medicine, contains about 200–300 species in the world [1]. In the Flora of Algeria, this genus is represented by 14 species of which four are endemic [2]. In continuation of our work on Stachys genus [3, 4, 5, 6], we report here the chemical constituents of the n-butanolic and dichloromethane extracts of the aerial parts of the Algerian species Stachys circinata L′Her. which has not been the subject of any previous study. Fifteen known compounds have been identified. We also report here the antibacterial and antioxidant activity of the n-butanolic extract by using disk diffusion and eletron spin resonance methods (ESR).The authors are grateful to the ATRSS and DGRSDT-MESRS (Algeria) for financial support.Peer Reviewe