Fast and Accurate Surface Normal Integration on Non-Rectangular Domains

International audienceThe integration of surface normals for the purpose of computing the shape of a surface in 3D space is a classic problem in computer vision. However, even nowadays it is still a challenging task to devise a method that is flexible enough to work on non-trivial computational domains with high accuracy, robustness, and computational efficiency. By uniting a classic approach for surface normal integration with modern computational techniques, we construct a solver that fulfils these requirements. Building upon the Poisson integration model, we use an iterative Krylov subspace solver as a core step in tackling the task. While such a method can be very efficient, it may only show its full potential when combined with suitable numerical preconditioning and problem-specific initialisation. We perform a thorough numerical study in order to identify an appropriate preconditioner for this purpose. To provide suitable initialisation, we compute this initial state using a recently developed fast marching integrator. Detailed numerical experiments illustrate the benefits of this novel combination. In addition, we show on real-world photometric stereo datasets that the developed numerical framework is flexible enough to tackle modern computer vision applications

The influence of bioreactor geometry and the mechanical environment on engineered tissues

A three phase model for the growth of a tissue construct within a perfusion bioreactor is examined. The cell population (and attendant extracellular matrix), culture medium, and porous scaffold are treated as distinct phases. The bioreactor system is represented by a two-dimensional channel containing a cell-seeded rigid porous scaffold (tissue construct), which is perfused with a culture medium. Through the prescription of appropriate functional forms for cell proliferation and extracellular matrix deposition rates, the model is used to compare the influence of cell density-, pressure-, and culture medium shear stress-regulated growth on the composition of the engineered tissue. The governing equations are derived in O'Dea et al. "A Three Phase Model for Tissue Construct Growth in a Perfusion Bioreactor," Math. Med. Biol., in which the long-wavelength limit was exploited to aid analysis; here, finite element methods are used to construct two-dimensional solutions to the governing equations and to investigate thoroughly their behavior. Comparison of the total tissue yield and averaged pressures, velocities, and shear stress demonstrates that quantitative agreement between the two-dimensional and long-wavelength approximation solutions is obtained for channel aspect ratios of order 10(-2) and that much of the qualitative behavior of the model is captured in the long-wavelength limit, even for relatively large channel aspect ratios. However, we demonstrate that in order to capture accurately the effect of mechanotransduction mechanisms on tissue construct growth, spatial effects in at least two dimensions must be included due to the inherent spatial variation of mechanical stimuli relevant to perfusion bioreactors, most notably, fluid shear stress, a feature not captured in the long-wavelength limit