Automatizace extrakce sulfamerazinu s využitím LOV techniky

Univerzita Karlova v Prahe Farmaceutická fakulta v Hradci Králové Katedra analytickej chémie Kandidát: Lucia Kováčová Školiteľ: Warunya Boonjob, Ph.D. Konzultant: Doc. PharmDr. Hana Sklenářová, Ph.D. Názov diplomovej práce: Automatizace extrakce sulfamerazinu s využitím LOV techniky Táto práca sa zaoberá vývojom metódy pre stanovenie sulfamerazínu v sére pomocou LOV metodiky. Analyt bol zakoncentrovaný na sorbente Oasis-HLB. Ako elučné činidlo bol použitý čistý methanol. K detekcii bola použitá UV spektrofotometria pri vlnovej dĺžke 275 nm. Boli nájdené optimálne podmienky pre automatizáciu v systéme SIA. Bola optimalizovaná tvorba kolonky, objem vzorky, rýchlosť aspirácie vzorky na kolonku, rýchlosť elúcie. Bola premerená kalibračná závislosť s korelačným koeficientom 0,99950 v rozsahu koncentrácií 50 - 1000 ppb. Bol vypočítaný limit detekcie (LOD = 71,70 µg/L) a limit kvantifikácie (LOQ = 238,99 µg/L). Premerala sa opakovateľnosť pre koncentrácie 500 ppb a 1000 ppb s relatívnymi smerodajnými odchýlkami (RSD) 5,66% a 6,60%.Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate: Lucia Kováčová Supervisor: Warunya Boonjob, Ph.D. Consultant: Doc. PharmDr. Hana Sklenářová, Ph.D. Title of diploma work: Automation of sulfamerazine extraction using LOV technique This theses deals with development of the method to determine sulfamerazine in spiked serum using the LOV methodology. The analyte was preconcentrated on sorbent Oasis- HLB. Pure methanol was used as eluent. UV spectrophotometry with wavelenght 275 nm was used as detection technique . The optimal conditions of measurement in SIA system were specified - the creation of microcolumn, the volume of analyte, the analyte aspiration and elution flowrates. The linear calibration curve was found with the correlation coeficient of 0.99950 in the concentration range of 50 - 1000 ppb. The limit of detection (LOD = 71.70 µg/L) and the limit of quantification (LOQ = 238.99 µg/L) were defined. The repeatability for concentrations of 500 ppb and 1000 ppb were measured. The relative standard deviations (RSDs) were 5.66% and 6.60%, respectively.Department of Analytical ChemistryKatedra analytické chemieFaculty of Pharmacy in Hradec KrálovéFarmaceutická fakulta v Hradci Králov

Evolution of depositional systems and sedimentary cycles in Želiezovce Depression of the Danube Basin

Five deep wells (Pozba4, Pozba3, Dubnik1, Nová Vieska1, Modrany1), drilled in the Želiezovce depression of the south-east Danube Basin penetrate the Cenozoic sedimentary record in overall thickness ranging from 1000 m up to 3000 m, the total thickness gradually increases from South to the North. This area is well known for the complex geological structure related to the Central Western Carpathians and Transdanubian Range junction along the Hurbanovo – Diösjenö line. Many authors have studied this area in the past,  nevertheless Depositional systems, sedimentary cycles and the development of sedimentary facies were never understood in detail. The Re-evaluation of biostratigraphy, paleoecology, sedimentology and the Re-interpretation of e-logs ware all incorporated into well correlations, what resulted in definition of four main sedimentary cycles: (1) The Lowermost Oligocene cycle, consisting of mudstones with occasional sandstone intercalations, associated with slope to offshore marine environment what is supported by the presence of the NP 22 calcareous nannoplankton zone. (2) The transgressive Langhian (Lower Badenian) deposits, composed of mudstones with epiclastic volcanic material and tuffite beds ware associated with shelfall to offshore environement the age is supported by the NN4 - NN5 calcareous nannoplankton zone. (3) The Serravallian (Upper Badenian - Sarmatian) cycle is composed mainly by mudstones with abundant Lithothamnium fragments associated with brackish coastal pain environment, supported by the NN6 calcareous nannoplankton zone and by the mass occurrence of fish scales and  Ervilia dissita (Eichwald) bivalves. (4) The overlying strata are represent the Tortonian-Pliocene (Pannonian-Pliocene) cycle, consists mostly from claystone which are overlain by sandstone and siltstones layers is associated with lake, deltaic and alluvial plain environment. 

Faktory ovplyvňujúce priemernú mzdu vo vybraných odvetviach v štátoch EÚ

The goal of this thesis is to identify and evaluate factors that significantly affect the average wage in health, education and administrative sectors in the EU based on quantification and verification of a multi-dimensional regulatory model. Two states are selected to be examined from all European countries, namely the Slovak and the Czech Republic. Those affecting factors are mainly macroeconomic (GDP, inflation, unemployment)

Propose an extension of security education at the primary school for child protection

Vedecká štúdia prináša výsledky návrhu rozšírenia obsahového zamerania bezpečnostného vzdelávania na ZŠ pre ochranu detí. Moderné a sofistikované metódy porušovania bezpečnostných pravidiel posunuli bezpečnostné vzdelávanie ešte viac do popredia záujmu verejnosti. To si vyžaduje neustálu analýzu zaistenia bezpečnosti detí aj v prostredí základných škôl a hľadanie nových možností ich ochrany pre ohrozeniami rôzneho druhu a spôsobu. Vychádzajúc z cieľa bezpečnostného vzdelávania v prostredí základných škôl boli v štúdii stanovené kompetencie (spôsobilosti) bezpečnostného konania a správania a v bežných i krízových situáciách absolventa bezpečnostného vzdelávania na základnej škole. Návrh vychádza práve zo stanovených kompetencií. V návrhu sú zároveň analyzované súčasné problémy, ktoré sú dôsledkom neustále sa meniacich spoločenských potrieb, ako aj rýchleho rozvoja vedy a techniky, najmä moderných masovo-komunikačných prostriedkov.The scientific study presents the proposal of extension of curriculum focused on security education for primary schools to children protection. Modern and sophisticated methods violation of safety rules, safety education moved even more to the forefront of the public interest. It requires continual analysis of ensuring the safety of children in the primary schools too and looking for new possibilities for protection against threats of various kinds and manner. The aim of security education determines competencies of security activity and behaviour in normal and emergency situations of school leavers. Proposal is based on these defined competencies. The proposal also includes an analysis of current problems, which are the consequences of constantly changing social needs, as well as the rapid development of science and technology, especially modern mass-communication

THE SIGNIFICANCE OF ENVIRONMENTAL EDUCATION IN THE CONTEXT OF INCREASING ENVIRONMENTAL ISSUES OF SOCIETY

The study brings an assessment of the significance of environmental education as one of the means of ensuring protecting of the environment. Environmental education is continually gaining importance due to the newly emerged environmental issues in society, particularly those with severe impact. One of the main goals of this study is to consider serious environmental issues of society and then analyse the significance of education in this area, as well as to classify ongoing forms of study programmes in particular stages of the educational system in The Slovak Republic

Automation of sulfamerazine extraction using LOV technique

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate: Lucia Kováčová Supervisor: Warunya Boonjob, Ph.D. Consultant: Doc. PharmDr. Hana Sklenářová, Ph.D. Title of diploma work: Automation of sulfamerazine extraction using LOV technique This theses deals with development of the method to determine sulfamerazine in spiked serum using the LOV methodology. The analyte was preconcentrated on sorbent Oasis- HLB. Pure methanol was used as eluent. UV spectrophotometry with wavelenght 275 nm was used as detection technique . The optimal conditions of measurement in SIA system were specified - the creation of microcolumn, the volume of analyte, the analyte aspiration and elution flowrates. The linear calibration curve was found with the correlation coeficient of 0.99950 in the concentration range of 50 - 1000 ppb. The limit of detection (LOD = 71.70 µg/L) and the limit of quantification (LOQ = 238.99 µg/L) were defined. The repeatability for concentrations of 500 ppb and 1000 ppb were measured. The relative standard deviations (RSDs) were 5.66% and 6.60%, respectively

Business Plan

Cieľom diplomovej práce je vypracovanie podnikateľského plánu na založenie nového ženského časopisu. Diplomová práca zahrňuje styri základné časti, a to úvod, teoreticko-metodologickú časť, praktickú časť a záver. Na začiatku je predstavenú úloha podnikateľského plánu v procese tvorby nového produktu. Zaoberá sa okrem jeho podstaty aj formou a štruktúrou a podrobne sú popísané jeho jednotlivé časti. Praktická časť spočíva v zostavení podnikatelského plánu pre založenie časopisu. Prevedená je základná analýza trhu pomocou PEST analýzy, analýza konkurenčního prostredia využitím Porterovho modelu piatich síl. Zhodnotenie projektu využíva SWOT analýzu a What – if analýzu.The diploma thesis is focused on a composition of the business plan for a comapny published a new magazine for the women. The thesis is consisted of four main parts, an introduction, part theoretical – methodological part, practical part and conclusion. The theoretical-methodological part is to explain the role, importance, structure and recommended process of designing business plans. It also contains description of the tools of chosen analysis, which are used in the practical part. The practical part of the thesis is to draw up the business plan of a new magazine for the women. In this part are made PEST analysis, Porter\u27s five forces analysis to understand the business enviroment and the market. In SWOT analysis and What – if analysis evaluate the new project

Further education of security community in the conditions of the sr

Štúdia pojednáva o ďalšom bezpečnostnom vzdelávaní na Slovensku, ktoré tvorí súčasť celoživotného vzdelávania. V rámci tohto bezpečnostného vzdelávania si účastník môže doplniť, rozšíriť a prehĺbiť si získané vedomosti, rekvalifikovať sa alebo uspokojiť svoje záujmy. Bezpečnostné vzdelávanie spočíva v analytickej činnosti, ktorá je zameraná na odvrátenie alebo minimalizáciu bezpečnostných rizík rôznej formy a príčiny voči jednotlivcom aj spoločnosti ako celkuThe study discusses further security education in Slovakia, which is part of lifelong learning. Within the framework of this security education the participant can complement, extend and deepen the knowledge acquired, to retrain or satisfy their interests. Security education is the analytical work aimed at averting or minimizing the security risks of different forms and causes to individuals and to society as a whole

Military Defence Training as an Element of Civil Defence Education in Former Czechoslovakia

Civil defence education in Czechoslovakia was one of the elements of education and the creation of conditions for every citizen to be physically and mentally capable and willing to fulfil his or her duties related to homeland defence. The aim of the paper is to examine the basic approaches to the implementation of military defence training as one of the basic elements of civil defence education in Czechoslovakia in the period from 1918 to 1989. The historical-comparative analysis is used as a method for researching military defence training issues. This method is treated as a qualitative research tool. The individual elements that military defence training included, such as exercising in nature, field orientation practice, and marching exercises are characterized. In military defence training in Czechoslovakia, emphasis was simultaneously placed on enabling children to be in touch with nature, to toughen them up and to train their observation skills. The training was an organizational form of school civil defence education. Its main aim was to train and prepare individuals, as well as schools as wholes, for emergencies

Incidence of Eating Disorders in Family Environment in High School Adolescents

AbstractThe impact of risk factors occurring in families on the adolescents’ attitude towards food, using the EAT-26 questionnaire and the Short (s)-EMBU was examined. Respondents were 464 high school students. Results show that in nearly 79% respondents there is at least one risk factor present in the family. Connection between a disturbed attitude to food and being on a family diet and failure to solve problems and absence of communication in families and the occurrence of psychic diseases in families was confirmed. The attachment was more disturbed on the father's side than on the mother's