Paraziter hastalıklarda serum demiri,total demir bağlama kapasitesi, transferlerinin saturasyonu ve serum ferritin değerleriniterilmesi

TEZ947Tez (Yüksek Lisans) -- Çukurova Üniversitesi, Adana, 1991.Kaynakça (s. 66-73) var.73 s. ; 30 cm.

Çukurova bölgesinde sıtmalılarda ve sıtma riski altında olanlarda İndirek Floresan Antikor Tekniği(IFAT) ile Plasmodium Vivax' a karşı oluşan antikorların gösterilmesi

TEZ1838Tez (Doktora) -- Çukurova Üniversitesi, Adana, 1995.Kaynakça (s. 41-45) var.46 s. ; 30 cm.

Investigation of intestinal parasites in the children of the Barbaros Primary School in the urban center in Adana

Bu çalışmada; Adana merkezinde sosyoekonomik düzeyi düşük bölgede bulunan Barbaros İlköğretim Okulundan 3 ile 8. sınıflar arasındaki çocuklarda bağırsak parazitlerinin yaygınlığını belirlemek amaçlandı. Dışkı ve selofanband örnekleri incelenen 482 öğrencinin 234 (%48.55}'ünde bir veya daha fazla parazit türü saptandı. Türlere göre parazit dağılımında; Enterobius vermicularis %37.97, Blastocystis hominis %9.33, Giardia lamblia %7.67, Enlamoeba coli %4.77 ve Hymenolepis nana %0.62 oranında saptandı.The aim of this study was to determine the prevalence of parasites in children in the 3-8 grades of the Barbaros Primary School in the urban center of Adana that has low socioeconomic level. Stool samples and cellophane-tape preparations of 482 students were examined and one or more intestinal parasites were detected in 234 (48.55%) out of 482 students. In this study; the rates of parasites were found as follows: Enterobius vermicularis 37.97%, Blastocystis hominis 9.33%, Giardia lamblia 7.67%, Enlamoeba coli 4.77%; and Hymenolepis nana 0.62%

Molecular Identification and DNA Sequencing of Trichomonas vaginalis Strains from Agean region of Turkey

The aim of this study is to compare the presence of Trichomonas vaginalis (T. vaginalis) in symptomatic and asymptomatic women through microscopic examination, culture in Trypticase-Yeast Maltose (TYM) medium and PCR methods. In addition, T. vaginalis strains were analysed for genotyping with 18S rRNA-DNA and phylogenetic analysis. Axenized strains of T. vaginalis isolated from urine culture samples taken from symptomatic and asymptomatic women with clinical signs. Molecular characterization of the isolated strains of T. vaginalis was performed by using PCR. To evaluate molecular diagnosis and genotypic identification of T. vaginalis strains, 14 samples were analysed. Of the 14 samples, T. vaginalis was positive in 14 samples by microscopy, 6 in culture(TYM medium) and 14 by PCR, respectively. Although the sample size is very small, PCR was shown to be high sensitivity and specificity, and seems to be a promising diagnostic tool. 18S rRNA-DNA PCR results also confirmed with real time PCR method. In conclusion, it is considered that two strains of T. vaginalis isolated from samples, 5-TV1G and 13-TV1G, are subtypes of T. vaginalis as a result of 18S rRNA-DNA sequencing analysis. To best of our knowledge this is the first analysis of phylogenetic positions on T. vaginalis from Turkey

Use of the ELISA (Em2-Em18) and Western Blotting Methods on Diagnosis of Alveolar Echinococcosis

Amaç: Alveolar ekinokokkoz (AE) kuzey yarım kürenin ölümcül parazitik zoonozlarından biri olup erken tanı konulması ve tedaviye başlanması hayatta kalma olasılığını arttırmaktadır. Bu çalışmada, çeşitli hastalıklardan tanı almış 50 hastanın serum örneği iki farklı serolojik tanı yöntemi ile değerlendirilmiştir. Yöntemler: Çalışmada, Em2-Em18 ELISA (Bordier Affinity Products, Crissier, Switzerland) ve Echinococcus Western Blot immünoglobulin G (IgG) (LDBIO Diagnostics, Lyon, France) yöntemleri kullanılmıştır. Bulgular: Em2-Em18 ELISA ile patolojik olarak AE tanılı 10 hastanın dokuzunda, kistik ekinokokkoz (KE) tanılı 21 hastanın ikisinde, 2 fascioliasis tanılı hastanın birinde ve bir kronik hepatitli hastada yüksek titrede antikor saptanmıştır. Doğrulayıcı test olarak kullanılan Echinococcus Western Blot IgG testi ile 10 AE hasta serumunun tamamı pozitif olarak değerlendirilirken KE hastalarının %85,7’sinde (18/21) IgG antikoru saptanmıştır. Hepatit hastasında ve kazeifiye granülomatöz enflamasyon görülen hastada ise bu yöntemle yanlış tanıya rastlanmıştır. Karaciğer ile ilgili hastalıkları olan ve diğer paraziter kaynaklı hastalıkları olan hastalardan alınan örnekler ise negatif olarak saptanmıştır. Sonuç: Çalışmada uygulanan serolojik yöntemler, endemik bölgelerde özellikle AE’nin erken tanısında ve sero-epidemiyolojik çalışmalarda kullanılabilirlik açısından önemli bulunmuştur.Objective: Alveolar echinococcosis (AE) is one of the most lethal parasitic zoonoses in the Northern Hemisphere, and early serological detection is important to start treatment and to improve survival. A total of 50 sera samples of patients diagnosed as having various diseases were examined for by two different serological diagnostic methods. Methods: Em2-Em18 ELISA (Bordier Affinity Products, Crissier, Switzerland) and Echinococcus Western Blot immünoglobulin G (IgG) (LDBIO Diagnostics, Lyon, France) were used for analyisis. Results: A high titer of antibodies was found in 9 of 10 patients diagnosed as having AE with Em2-Em18 ELISA, in 2 of 21 patients with cystic echinococcosis, in 1 of 2 patients with fascioliasis and in 1 patient with chronic hepatitis. the Echinococcus Western Blot IgG test, used as a confirmatory test, showed IgG antibody in 85.7% (18/21) of patients with CE, while all serum samples of 10 patients with AE were evaluated as positive. This method yielded an incorrect diagnosis in the patient with chronic hepatitis and in the patient with granulomatous inflammation with caseification. Samples taken from patients with liver-related diseases and other parasitic-related diseases were found to be negative. Conclusion: the serological methods used in the study were found to be important in the early diagnosis of alveolar echinococcosis in the endemic areas, since it could be used in sero-epidemiological studies

Oxidative Stress in Vivax Malaria

Malaria is still a leading cause of morbidity and mortality. The increase in lipid peroxidation reported in malaria infection and antioxidant status may be a useful marker of oxidative stress during malaria infection. The aim of this study was to investigate the role of antioxidant enzymes against toxic reactive oxygen species in patients infected with Plasmodium vivax and healthy controls. Malondialdehyde levels, superoxide dismutase, and glutathione peroxidase activities were determined in 91 P vivax patients and compared with 52 controls. Malondialdehyde levels, superoxide dismutase, and glutathione peroxidase activities were 8.07 +/- 2.29 nM/ml, 2.69 +/- 0.33 U/ml, and 49.6 +/- 3.2 U/g Hb in the patient group and 2.72 +/- 0.50 nM/ml, 3.71 +/- 0.47 U/ml, and 62.3 +/- 4.3 U/g Hb in the control group, respectively. Malondialdehyde levels were found statistically significant in patients with vivax malaria higher than in healthy controls (P<0.001). On the other hand, superoxide dismutase and glutathione peroxidase activities were found to be significantly lower in vivax malaria patients than in controls (P<0.05). There was an increase in oxidative stress in vivax malaria. The results suggested that antioxidant defense mechanisms may play an important role in the pathogenesis of P vivax

Bir kutanöz leishmaniasis endemik bölgesi olan Adana’nın Karaisalı ilçesinde kum sineği faunası ve çevresel parametreleri

Purpose: This paper presents the results of an entomological survey in an endemic focus of cutaneous leishmaniasis in Karaisali province of Adana in Turkey. Materials and Methods: There were two field works in two consecutive years (2013 and 2014), which 1088 sand fly specimens were captured using 136 light traps which were conducted in 11 villages of Karaisali located in southwest part of Adana. Results: Totally, six Phlebotomus species were described: Phlebotomus tobbi (41.54%), Phlebotomus neglectus/syriacus (9.74%), Phlebotomus papatasi (25.18%), Phlebotomus perfiliewi (3.49%), Sergentomyia dentata (18.38%) and Sergentomyia theodori (1.65%). The female/male rate was found to be 1.98. Phlebotomus tobbi, Phlebotomus papatasi, Phlebotomus neglectus/syriacus, Sergentomyia dentata and Sergentomyia theodori were dominant at 201-400m, 0-200m, 801-1000m, 401-600m and 601-800m respectively. Conclusion: Environmental and climatic factors were compared for the presence of sand flies species especially vectors of cutaneous leishmaniasis by univariate binary regression analysis in PASW. Sand fly density maps were generated based on the elevation they were caught by using ARCMAP 10.2. The data also revealed a relationship between presence of the probable vector sand flies and several environmental factors such as altitude, temperature, trap location and humidity in KaraisaliAmaç: Bu çalışmanın amacı, bir endemik bölge olan Adana'nın Karaisalı ilçesindeki kutanöz leishmaniasisin endemik odaklı entomolojik bir araştırmasının sonuçlarını sunmaktır. Gereç ve Yöntem: Adana'nın güneybatısında bulunan Karaisalı'nın 11 köyünde İki yıl üst üste iki alanda (2013 ve 2014), 136 ışık tuzağı kullanılarak 1088 kum sineği örneği toplanmış, saha ile ilgili çevresel veriler kaydedilmiştir. Ayrıca Örnek toplanan alanlardaki çevresel ve iklimsel faktörler, vektör kum sineklerinin varlığı ile PASW'da tek değişkenli ikili regresyon yöntemi kullanılarak karşılaştırma yapıldı. Kum sineklerinin dağılım haritaları, ARCMAP 10.2 kullanılarak yakalandıkları yükseklere bağlı olarak oluşturuldu. Bulgular: Toplanan örneklerin türleri; Phlebotomus tobbi % 41,54), Phlebotomus neglectus/syriacus (% 9,74), Phlebotomus papatasI % 25,18), Phlebotomus perfiliewi (% 3.49), Sergentomyia theodori (% 10,56) ve Sergentomyi adentata(% 9,46) olarak belirlenmiştir. Erkek/dişi oranı ise 1.89 olarak bulunmuştur. Phlebotomus.tobbi, Phlebotomus papatasi, Phlebotomus neglectus/syriacus, Sergentomyia ia dentata ve Sergentomyia theodori sırasıyla 201-400m, 0-200m, 801-1000m, 401-600m ve 601-800m yüksekliklerde baskın t ürler olarak belirlenmiştir. Sonuç: Veriler, muhtemel vektör kum sineklerinin varlığı ile Karaisalı'da yükseklik, sıcaklık, tuzak konumu ve nem gibi birkaç çevresel faktör arasında bir ilişki olduğunu ortaya koymuştur

A View of Isosporiosis with an Immunocompromised Patient

Because chronic diarrhea in patients infected with human immunodeficiency virus (HIV) results in a significant morbidity and mortality, the early detection and proper treatment of patients with AIDS and concomitant diarrhea is very important in preventing complications and prolonging a healthy life. Diarrhea due to Isospora belli is reviewed by evaluating a 29 year-old HIV infected patient diagnosed in our clinic. Isosporiasis should be suspected in HIV infected patients from tropical and subtropical countries with either acute or chronic watery diarrhea and weight loss

Cystic echinococcosis in Turkey from 2001-2005

Echinococcus granulosus’un metasestod formunun ara konaklarda sebep olduğu kistik ekinokokkozis (KE) dünyada özellikle de hayvancılığın yaygın olduğu bölgelerde büyük bir halk sağlığı problemidir. Çalışmamızda, Türkiye’de 2001-2005 yıllarında değişik hastanelerden, İl Sağlık Müdürlüklerinden ve Sağlık Bakanlığı’ndan elde edilen kayıtların retrospektif olarak gözden geçirilmesiyle saptanan KE olguları incelenmiştir. Elde edilen sonuçlara göre Marmara Bölgesi’nde 2534 (%13,13), Ege Bölgesi’nde 2114 (%16,94), Akdeniz Bölgesi’nde 2578 (%16,09), İç Anadolu Bölgesi’nde 5404 (%38,57), Karadeniz Bölgesi’nde 428 (%5.70), Doğu Anadolu Bölgesi’nde 844 (%6,80), Güneydoğu Anadolu Bölgesi’nde 887 (%2,75) olmak üzere toplam 14789 KE olgusu saptanmıştır. Saptanan olguların toplam 149464 gün hastanede yattığı belirlenmiştirCystic echinococcosis (CE) caused by the metacestode form of Echinococcus granulosus is a major public health problem especially in animal-raising regions of the world. In the present study, CE cases were determined during 2001-2005 by investigating different hospital and health directorship documents and Health Ministry documents, retrospectively. Our results show that there were 2534 (13.13%) cases in the Marmara region; 2114 (16.94%), in the Aegean region; 2578 (16.09%), Mediterranean region; 5404 (38.57%), in the Middle Anatolian region; 428 (5.70%), in the Black Sea region; 844 (6.80%), in the eastern Anatolian region; and 887 (2.75%), in the southeastern Anatolian region making a total of 14,789 CE cases. Finally, it has been determined that the patients were hospitalized for a total of 149,464 days