Occurrence and Quantification of Antimicrobial Resistance Genes in the Gastrointestinal Microbiome of Two Wild Seabird Species With Contrasting Behaviors

Indexación ScopusAntimicrobial resistance genes (ARGs) are environmental pollutants and anthropization indicators. We evaluated human interference in the marine ecosystem through the ocurrence and quantification (real-time PCRs) of 21 plasmid-mediated ARGs in enema samples of 25 wild seabirds, upon admission into rehabilitation: kelp gull (Larus dominicanus, n = 14) and Magellanic penguin (Spheniscus magellanicus, n = 11). Overall, higher resistance values were observed in kelp gulls (non-migratory coastal synanthropic) in comparison with Magellanic penguins (migratory pelagic non-synanthropic). There were significant differences between species (respectively, kelp gull and Magellanic penguin): ARGs occurrence (blaTEM [p = 0.032]; tetM [p = 0.015]; tetA [p = 0.003]; and sulII [p = 0.007]), mean number of ARGs per sample (p = 0.031), ARGs mean load percentage (aadA [p = 0.045], tetA [p = 0.031], tetM [p = 0.016], blaTEM [p = 0.032], sulII [p = 0.008]), percentage of genes conferring resistance to an antimicrobial class (betalactams [p = 0.036] and sulfonamides [p = 0.033]), mean number of genes conferring resistance to one or more antimicrobial classes (p = 0.024]), percentage of multiresistant microbiomes (p = 0.032), and clustering (p = 0.006). These differences are likely due to these species' contrasting biology and ecology - key factors in the epidemiology of ARGs in seabirds. Additionally, this is the first report of mecA in seabirds in the Americas. Further studies are necessary to clarify the occurrence and diversity of ARGs in seabirds, and their role as potential sources of infection and dispersal within the One Health chain of ARGs. © Copyright © 2021 Ewbank, Esperón, Sacristán, Sacristán, Neves, Costa-Silva, Antonelli, Rocha Lorenço, Kolesnikovas and Catão-Dias.https://www.frontiersin.org/articles/10.3389/fvets.2021.651781/ful

Bioquímica plasmática de cascavéis (Caudisona durissa LINNAEUS, 1758) em cativeiro Blood biochemical parameters from captivity rattlesnake (Caudisona durissa LINNAEUS, 1758)

Acidentes com seres humanos envolvendo a espécie Caudisona durissa possuem a mais alta taxa de letalidade dentre os viperídeos brasileiros. Ressalta-se então a importância para a saúde pública da criação dessa espécie em cativeiro para produção de soro antiofídico. No entanto, essa atividade ainda hoje encontra alguns desafios como a instalação de doenças no plantel, evidenciando a importância de estudos sobre a fisiologia de serpentes. Dessa forma, foram realizadas análises de bioquímica plasmática em 53 serpentes da espécie Caudisona durissa, mantidas em cativeiro. Foram utilizadas amostras de plasma com heparina e as dosagens bioquímicas realizadas em aparelho automatizado (Ciba Corning - Express Plus®). Os resultados e seus respectivos desvios-padrões foram: uréia -1,32mg dL-1 (&plusmn;1,1); ácido úrico - 2,08mg dL-1 (&plusmn;1,4); creatinina - 0,52mg dL-1 (&plusmn;0,2); proteína total - 3,7g dL-1 (&plusmn;0,7); albumina - 1,62g dL-1 (&plusmn;0,4); globulinas - 2,08g dL-1 (&plusmn;0,5); cálcio - 15,25mg dL-1 (&plusmn;2,8); fósforo - 4,61mg dL-1 (&plusmn;1,9); colesterol - 171,58mg dL-1 (&plusmn;52,7); triglicerídeos - 19,29mg dL-1 (&plusmn;14,3); fosfatase alcalina - 31,04U L-1 (&plusmn;12,4); aspartato aminotransferase (AST) - 22,25U L-1 (&plusmn;11,4); alanina aminotransferase (ALT) - 7,11U L-1 (&plusmn;5,4) e Amilase - 1385,23U L-1 (&plusmn;568,7). Foram calculados os seguintes índices: relação uréia/creatinina - 2,5 e relação cálcio/fósforo - 3,3. O conjunto de resultados das análises bioquímicas do sangue das cascavéis em cativeiro pode servir como referência para apoio diagnóstico na espécie estudada e para outros trabalhos com o mesmo caráter de observação.<br>Human accidents involving rattlesnake Caudisona durissa have the highest fatality rate among the Brazilian Viperidae family. Breeding this specie in captivity in order to produce antivenoms is very important to public health. Nevertheless, there are some challenges that this activity must face, like the onset of diseases on the breeding stock, justifying the importance of studying these snakes' physiology. Blood biochemical analysis of 53 Caudisona durissa was performed using plasma samples in an automated equipment (Ciba Corning - Express Plus®). Mean values and its respective Standard deviations are: blood urea nitrogen (BUN) -1.32mg dL-1 (&plusmn;1.1); uric acid - 2.08mg dL-1 (&plusmn;1.4); creatinine - 0.52mg dL-1 (&plusmn;0.2); total protein - 3.7g dL-1 (&plusmn;0.7); albumin - 1.62g dL-1 (&plusmn;0.4); globulins - 2.08g dL-1 (&plusmn;0.5); calcium - 15.25mg dL-1 (&plusmn;2.8); phosphorus - 4.61mg dL-1 (&plusmn;1.9); cholesterol - 171.58mg dL-1 (&plusmn;52.7); triglycerides - 19.29mg dL-1 (&plusmn;14.3); alkaline phosfatase - 31.04U L-1 (&plusmn;12.4); aspartate aminotransferase (AST) - 22.25U L-1 (&plusmn;11.4); alanin aminotransferase (ALT) 7.11U L-1 (&plusmn;5.4); amylases - 1385.23U L-1 (&plusmn;568.7). The following indexes were calculated: BUN/creatinine ratio - 2.5 and calcium/phosphorus ratio -3.3. Rattlesnake blood biochemical results above could be used as reference in clinical evaluation for captivity C. durissa

Pulmonary, microbiological and hematological changes in Crotalus durissus terrificus (Serpentes, Viperidae) parasitized by nematodes of the genus Rhabdias (Nematoda, Rhabdiasidae) Alterações Pulmonares, microbiológicas e hematológicas em Crotalus durissus terrificus (Serpentes, Viperidae) parasitadas pelos nematódeos do gênero Rhabdias (Nematoda, Rhabdiasidae)

This study reported the pulmonary, microbiological, and hematological alterations in Crotalus durissus terrificus parasitized by nematodes of the genus Rhabdias. Histological, microbiological, and hematological analysis were performed on parasitized (n=6) and non-parasitized (n=6) snakes. Granulocytic and mononuclear cell infiltrates in the pulmonary parenchyma and epithelium were also observed during the histological analysis of parasitized snakes. Microbiological analysis of parasitized animals revealed the following Gram-negative bacteria: Citrobacter divergens, Burkholderia cepacia, Stenotrophomonas maltophilia, Proteus vulgaris, Enterobacter sakazakii, Enterobacter ammnigenus, Pseudomonas aeruginosa, Pantoea sp. and Providencia rettgeri. In non-parasitized snakes, the following species were identified: B. cepacia, Pseudomonas fluorescens, and Acinetobacter baumanii. Increased levels of plasmatic protein, decreased lymphocyte counts, and normal red blood cell values were observed in parasitized animals.<br>Este trabalho relata as alterações pulmonares, microbiológicas e hematológicas em Crotalus durissus terrificus parasitadas pelo nematódeo do gênero Rhabdias. As análises histológicas, microbiológicas e hematológicas foram realizadas em serpentes parasitadas (n=6) e não parasitadas (n=6). Foram observados infiltrados de células granulocíticas e mononucleares no parênquima pulmonar durante a análise histopatológica das serpentes parasitadas. A análise microbiológica revelou as seguintes bactérias Gram-negativas; Citrobacter divergens, Burkholderia cepacia, Stenotrophomonas maltophilia, Proteus vulgaris, Enterobacter sakazakii, Enterobacter ammnigenus, Pseudomonas aeruginosa, Pantoea sp. e Providencia rettgeri. Nas serpentes não parasitadas foram identificadas: B. cepacia, Pseudomonas fluorescens e Acinetobacter baumanii. Nos animais parasitados observaram-se: aumento da concentração de proteína plasmática, diminuição da contagem de linfócitos, e valores normais de hematimetria