Gypsy Moth (Lepidoptera: Lymantriidae) Feeding on Purple Loosestrife \u3ci\u3e(Lythrum Salicaria)\u3c/i\u3e in Michigan

Purple loosestrife, Lythrum salicaria, is an exotic invasive weed which is currently the target of a biological control effort using introduced leaf-feeding beetles. In 1997-1998 we observed larvae of the gypsy moth, Lymantria dispar feeding on L. salicaria at several locations in south central Michigan. In one-minute timed counts conducted over a six-week period in 1998, densities of 0 to 8 larvae per 1-m2 quadrat were observed. Other observations indicated 23 L. dispar 2nd and 3rd instars on a single L. salicaria plant. Second and third instar L. dispar collected on L. salicaria in the field were successfully reared to the adult stage in the lab on a diet of L. salicaria foliage. This is the first report of L. dispar feeding and development on L. salicaria. In areas where they co-occur, distinguishing L. dispar damage from that of introduced natural enemies will be important so that estimates of biocontrol agent impact are not biased

Thermodynamically accurate modeling of the catalytic cycle of photosynthetic oxygen evolution: A mathematical solution to asymmetric Markov chains

AbstractForty-three years ago, Kok and coworkers introduced a phenomenological model describing period-four oscillations in O2 flash yields during photosynthetic water oxidation (WOC), which had been first reported by Joliot and coworkers. The original two-parameter Kok model was subsequently extended in its level of complexity to better simulate diverse data sets, including intact cells and isolated PSII-WOCs, but at the expense of introducing physically unrealistic assumptions necessary to enable numerical solutions. To date, analytical solutions have been found only for symmetric Kok models (inefficiencies are equally probable for all intermediates, called “S-states”). However, it is widely accepted that S-state reaction steps are not identical and some are not reversible (by thermodynamic restraints) thereby causing asymmetric cycles. We have developed a mathematically more rigorous foundation that eliminates unphysical assumptions known to be in conflict with experiments and adopts a new experimental constraint on solutions. This new algorithm termed STEAMM for S-state Transition Eigenvalues of Asymmetric Markov Models enables solutions to models having fewer adjustable parameters and uses automated fitting to experimental data sets, yielding higher accuracy and precision than the classic Kok or extended Kok models. This new tool provides a general mathematical framework for analyzing damped oscillations arising from any cycle period using any appropriate Markov model, regardless of symmetry. We illustrate applications of STEAMM that better describe the intrinsic inefficiencies for photon-to-charge conversion within PSII-WOCs that are responsible for damped period-four and period-two oscillations of flash O2 yields across diverse species, while using simpler Markov models free from unrealistic assumptions

High Brain Ammonia Tolerance and Down-Regulation of Na+:K+:2Cl- Cotransporter 1b mRNA and Protein Expression in the Brain of the Swamp Eel, Monopterus albus, Exposed to Environmental Ammonia or Terrestrial Conditions

10.1371/journal.pone.0069512PLoS ONE89-POLN

Quantification of non-Q B -reducing centers in leaves using a far-red pre-illumination

An alternative approach to quantification of the contribution of non-QB-reducing centers to Chl a fluorescence induction curve is proposed. The experimental protocol consists of a far-red pre-illumination followed by a strong red pulse to determine the fluorescence rise kinetics. The far-red pre-illumination induces an increase in the initial fluorescence level (F25 μs) that saturates at low light intensities indicating that no light intensity-dependent accumulation of QA− occurs. Far-red light-dose response curves for the F25 μs-increase give no indication of superimposed period-4 oscillations. F25 μs-dark-adaptation kinetics following a far-red pre-pulse, reveal two components: a faster one with a half-time of a few seconds and a slower component with a half-time of around 100 s. The faster phase is due to the non-QB-reducing centers that re-open by recombination between QA− and the S-states on the donor side. The slower phase is due to the recombination between QB− and the donor side in active PS II reaction centers. The pre-illumination-induced increase of the F25 μs-level represents about 4-5% of the variable fluorescence for pea leaves (∼2.5% equilibrium effect and 1.8-3.0% non-QB-reducing centers). For the other plant species tested these values were very similar. The implications of these values will be discusse

Insights into substrate binding to the oxygen-evolving complex of photosystem II from ammonia inhibition studies

© 2014 American Chemical Society. Water oxidation in Photosystem II occurs at the oxygen-evolving complex (OEC), which cycles through distinct intermediates, S0-S4. The inhibitor ammonia selectively binds to the S2 state at an unresolved site that is not competitive with substrate water. By monitoring the yields of flash-induced oxygen production, we show that ammonia decreases the net efficiency of OEC turnover and slows the decay kinetics of S2 to S1. The temperature dependence of biphasic S2 decay kinetics provides activation energies that do not vary in control and ammonia conditions. We interpret our data in the broader context of previous studies by introducing a kinetic model for both the formation and decay of ammonia-bound S2. The model predicts ammonia binds to S2 rapidly (t1/2 = 1 ms) with a large equilibrium constant. This finding implies that ammonia decreases the reduction potential of S2 by at least 2.7 kcal mol-1 (\u3e120 mV), which is not consistent with ammonia substitution of a terminal water ligand of Mn(IV). Instead, these data support the proposal that ammonia binds as a bridging ligand between two Mn atoms. Implications for the mechanism of O-O bond formation are discussed

Photosynthetic water oxidation: Binding and activation of substrate waters for O-O bond formation

© 2015 The Royal Society of Chemistry. Photosynthetic water oxidation occurs at the oxygen-evolving complex (OEC) of Photosystem II (PSII). The OEC, which contains a Mn4CaO5 inorganic cluster ligated by oxides, waters and amino-acid residues, cycles through five redox intermediates known as Si states (i = 0-4). The electronic and structural properties of the transient S4 intermediate that forms the O-O bond are not well understood. In order to gain insight into how water is activated for O-O bond formation in the S4 intermediate, we have performed a detailed analysis of S-state dependent substrate water binding kinetics taking into consideration data from Mn coordination complexes. This analysis supports a model in which the substrate waters are both bound as terminal ligands and react via a water-nucleophile attack mechanism