Attempts to increase the rate of organ donation confirmation at emergency medical department

富山大学・富医薬博甲第328号・高橋 絹代・2020/03/24関連論文Importance of Continuing Education for Medical Staff to Improve the Confirmation Rate of Intent for Organ Donationdoi：10.1016/j.transproceed.2019.08.043富山大

Myelin Basic Protein as a Novel Genetic Risk Factor in Rheumatoid Arthritis—A Genome-Wide Study Combined with Immunological Analyses

Rheumatoid arthritis (RA) is a major cause of adult chronic inflammatory arthritis and a typical complex trait. Although several genetic determinants have been identified, they account for only a part of the genetic susceptibility. We conducted a genome-wide association study of RA in Japanese using 225,079 SNPs genotyped in 990 cases and 1,236 controls from two independent collections (658 cases and 934 controls in collection1; 332 cases and 302 controls in collection2), followed by replication studies in two additional collections (874 cases and 855 controls in collection3; 1,264 cases and 948 controls in collection4). SNPs showing p<0.005 in the first two collections and p<10−4 by meta-analysis were further genotyped in the latter two collections. A novel risk variant, rs2000811, in intron2 of the myelin basic protein (MBP) at chromosome 18q23 showed strong association with RA (p = 2.7×10−8, OR 1.23, 95% CI: 1.14–1.32). The transcription of MBP was significantly elevated with the risk allele compared to the alternative allele (p<0.001). We also established by immunohistochemistry that MBP was expressed in the synovial lining layer of RA patients, the main target of inflammation in the disease. Circulating autoantibody against MBP derived from human brain was quantified by ELISA between patients with RA, other connective tissue diseases and healthy controls. As a result, the titer of anti-MBP antibody was markedly higher in plasma of RA patients compared to healthy controls (p<0.001) and patients with other connective tissue disorders (p<0.001). ELISA experiment using citrullinated recombinant MBP revealed that a large fraction of anti-MBP antibody in RA patients recognized citrullinated MBP. This is the first report of a genetic study in RA implicating MBP as a potential autoantigen and its involvement in pathogenesis of the disease

An Estimate of the Velocity and Stress in the Deep Ocean Bottom Boundary Layer

In order to estimate the near-bottom velocity and the boundary shear stress, a one-dimensional unsteady boundary layer model was applied for the observed time series of the 6-level current observation conducted from Oct. 26 through to Nov. 3, 1988 at the site of the north western end of the Kyushu-Palau Ridge. Current meters were deployed 6, 10.5, 18.5, 39.0, 75.0 and 151.0 meters respectively up from the sea floor of 3519 m depth for seven days. The applied model, developed by McLean and Yean, predicts fairly well the near-bottom velocity field and stress, with the adjusted roughness of zo=0.025 cm. The estimated time-averaged drag coefficient is 4.5 x 10-3 • Thickness of the logarithmic layer is inferred as the order of 1 meter, which overlies the viscous sublayer of a thickness of about 1 cm. 1

Visualization and Measurements of Sub-Millisecond Transient Spray Dynamics Applicable to Direct Injection Gasoline Engine : Part 1:Visualization of Spray Using High Speed Video Camera and Laser Sheet Technique

Transient temporal and spatial spray characteristics have been investigated. A kind of practical fuel injector with swirl nozzle was examined under variable injection duration within the range of 0.25 to 4.0 ms and injection pressure 5.0 and 7.0 MPa. The fuel spray was injected at a cycling frequency of 50 Hz in a room under normal atmospheric condition. Considered details of sub-millisecond transient dynamics were observed by using a high-speed video camera with temporal resolution of 24.69 μs(40500 frames / s). The results indicate that investigated spray consists of two incorporated flow fractions that are a core jet and a quasi-umbrella droplets spray simultaneously moving down and swirling in local cross-axis domains. Instantaneous transient values of axial and radial penetration were accurately evaluated and analyzed. It was observed that after actual injection a post-injection of fuel droplets occurs and the time-delay and lifetime of these droplets were systematically estimated

Direct Delivery of Cas9-sgRNA Ribonucleoproteins into Cells Using a Nanoneedle Array

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is a powerful and widely used tool for genome editing. Recently, it was reported that direct delivery of Cas9-sgRNA ribonucleoproteins (RNPs) reduced off-target effects. Therefore, non-invasive, high-throughput methods are needed for direct delivery of RNPs into cells. Here, we report a novel method for direct delivery of RNPs into cells using a nanostructure with a high-aspect-ratio and uniform nanoneedles. This nanostructure is composed of tens of thousands of nanoneedles laid across a 2D array. Through insertion of the nanoneedle array previously adsorbed with Cas9-sgRNA, it was possible to deliver RNPs directly into mammalian cells for genome editing