Berberine Attenuates Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage Via Modulating AMPK/Rho Pathway

Purpose: Our goal is to clarify the effectiveness of berberine (BBR) on cerebral vasospasm induced by subarachnoid hemorrhage

Assessment of atherosclerosis in obese adolescents: Positive correlation of mean platelet volume and carotid intima media thickness

Aims: This study aims to assess the correlation of mean platelet volume (MPV) and common carotid artery (CCA) thickness in a population of obese adolescents

Does intra-articular fracture change the lubricant content of synovial fluid?

Background: Lubrication function is impaired and the lubricant content of synovial fluid (SF) changes immediately after plateau tibia fractures. Here, we aimed to analyze the lubricant content of SF at chronic term following plateau tibia fracture

Investigation of dose-dependent effects of berberine against renal ischemia/reperfusion injury in experimental diabetic rats

Background: Ischemia-reperfusion injury causes various severe morphological and functional changes in diabetic patients. To date, numerous antidiabetic and antioxidant agents have been used for treatment of the disease-related changes

Oxidative Stress in Children with Chronic Spontaneous Urticaria.

The pathogenesis of chronic spontaneous urticaria (CSU) has not been fully understood; nevertheless, significant progress has been achieved in recent years. The aim of this study was to investigate the possible role of reactive oxygen species (ROS) in the pathogenesis of CSU. Sixty-two children with CSU and 41 healthy control subjects were enrolled in the study. An extensive evaluation of demographic and clinical features was done, and serum oxidative stress was evaluated by plasma total oxidant status (TOS) and total antioxidant status (TAS) measurements. The median value of plasma TOS was found to be 10.49 mu mol H2O2 equiv./L (interquartile range, 7.29-17.65) in CSU patients and 7.68 mu mol H2O2 equiv./L (5.95-10.39) in the control group. The difference between the groups was statistically significant (p = 0.003). Likewise, the median plasma TAS level in the CSU group was decreased significantly compared to that of the control group (2.64 [2.30-2.74] versus 2.76 [2.65-2.86] mmol Trolox equiv./L, resp., p = 0,001). Our results indicated that plasma oxidative stress is increased in children with CSU when compared to healthy subjects, and plasma oxidative stress markers are positively correlated with disease activity

SP057EFFECTS OF PROXEED PLUS IN THE PREVENTION AND TREATMENT OF CISPLATIN NEPHROTOXICITY: AN EXPERIMENTAL RAT STUDY

INTRODUCTION AND AIMS:Cisplatin is widely used in solid organ cancer chemotherapy. One of the most important side effects of cisplatin is nephrotoxicity. The antioxidant properties of Proxeed Plus (PP) molecules and minerals provide a significant increase in sperm count and function. In our study, the effect of Proxeed Plus was evaluated histopathologically and biochemically in the prevention and treatment of cisplatin nephrotoxicity.METHODS:In our study, 40 rats were included. The rats were divided into five groups of (CIS), (PP+CIS), (PP+CIS+PP), (PP), (Control). At the end of the treatment, the rats were sacrified and intracardiac blood samples and kidney tissues were taken. Renal damage markers (BUN, creatinine), oxidative stress parameters (TAS, TOS, OSI), DNA damage, proinflammatory cytokines (TNF-alpha, IL-1 Beta, IL-6) and antiinflammatory cytokine (IL-10) were measured by biochemical evaluation. Histopathological evaluation of renal structure was studied with haematoxylin eosin. In addition, the apoptosis was examined by TUNEL method and caspase 3.RESULTS:Only in the cisplatin group were present higher BUN, creatinine, TOS, OSI, DNA damage, proinflammatory cytokines, and lower TAS and IL-10 values than the other groups. Although, BUN, creatinine, TOS, OSI, DNA damage, proinflammatory cytokines values were significantly lower in the group treated with PP than cisplatin group, values were significantly higher than control group. The BUN, creatinine, TOS, OSI, DNA damage, proinflammatory cytokines values of the group, which used PP before and after cisplatin, were significantly lower than those of both group used only cisplatin and used PP before cisplatin; There was no significant difference with the control group. Histopathological evaluation showed significant improvement in haematoxylin eosin staining compared to only cisplatin treated group than in both groups treated with PP. In the TUNEL and caspase 3 staining, in which apoptosis was assessed, the apoptosis rate was significantly lower in the group treated with Proxeed plus before and after cisplatin than in the group treated with both PP before cisplatin and cisplatin alone.CONCLUSIONS:The effect of Proxeed plus, which contains several molecules of nephrotoxic activity in previous studies, on cisplatin nephrotoxicity was investigated for the first time. The use of Proxeed plus before and after cisplatin has been demonstrated by both biochemical and histopathological mechanisms that prevent nephrotoxic effect

Elevated Nitrosative Stress in Children with Chronic Spontaneous Urticaria

Background: Chronic spontaneous urticaria (CSU) is an idiopathic condition that seriously affects quality of life. It is well known that oxidative stress and nitrosative stress (NS) are generally involved in many chronic inflammatory diseases. This study aimed to evaluate the possible role of NS in the pathogenesis of CSU. Methods: Thirty-two children with CSU and 22 healthy control subjects were enrolled in the study. Demographic and clinical features were defined, and disease activity was quantified using the urticaria activity score (UAS). Serum NS was assessed by the plasma levels of total nitric oxide (NOx) metabolites and nitrite and nitrate measurements using a Griess method -based commercial kit. Results: Plasma NOx levels were 82.5 11.3 mol/L in the CSU group and 50.9 9.4 mol/L in the control group. The difference was statistically significant (p < 0.001). CSU patients also had higher plasma nitrite levels than controls (53.3 13.8 vs. 30.2 10.1 mol/L, respectively, p < 0.001). The median values of plasma nitrate were 27.5 mon (IQR 19.1-35.5) in CSU patients and 20.9 rnol/L (IQR 17.9-23.2) in the control group, and the difference was statistically significant (p = 0.009). In addition, plasma NOx and nitrite levels were positively correlated with the UAS (rho = 0.512, p = 0.03 and rho = 0.452, p = 0.011, respectively). Conclusion: Plasma NS is elevated and positively correlated with disease activity in children with CSU. (C) 2017 S. Ka rger AG, Base