Requirement of Podocalyxin in TGF-Beta Induced Epithelial Mesenchymal Transition

Epithelial mesenchymal transition (EMT) is characterized by the development of mesenchymal properties such as a fibroblast-like morphology with altered cytoskeletal organization and enhanced migratory potential. We report that the expression of podocalyxin (PODXL), a member of the CD34 family, is markedly increased during TGF-β induced EMT. PODXL is enriched on the leading edges of migrating A549 cells. Silencing of podocalyxin expression reduced cell ruffle formation, spreading, migration and affected the expression patterns of several proteins that normally change during EMT (e.g., vimentin, E-cadherin). Cytoskeletion assembly in EMT was also found to be dependent on the production of podocalyin. Compositional analysis of podocalyxin containing immunoprecipitates revealed that collagen type 1 was consistently associated with these isolates. Collagen type 1 was also found to co-localize with podocalyxin on the leading edges of migrating cells. The interactions with collagen may be a critical aspect of podocalyxin function. Podocalyxin is an important regulator of the EMT like process as it regulates the loss of epithelial features and the acquisition of a motile phenotype

Defining Polyamory: A Thematic Analysis of Lay People’s Definitions

This study aimed to analyze laypeople’s definitions of polyamory and compare definitions presented by people who are not willing to engage in consensual non-monogamy (CNM) and those who are or are willing to be in a CNM relationship. This exploratory qualitative study used data collected from a convenience sample through a web survey, where people answered the question “What does polyamory mean?” We conducted thematic analysis to examine patterns in meaning and used demographic data to compare themes among groups. The final sample comprised 463 participants aged 18–66 years (M = 32.19, SD = 10.02), mostly heterosexual (60%). Of the total sample, 54% were in a monogamous relationship, followed by 21% not in a relationship, and 13% in a non-monogamous relationship. Analysis showed that people define polyamory mostly as a set of behaviors in a relationship, followed by the potential of multiple relationships or feelings for multiple people. Definitions also include emotional, sexual, and ethical aspects. People in CNM relationships are more likely to define polyamory as constituting a potential form of relating, focus more on interpersonal feelings and ethics, and include consent in their definitions than those unwilling to engage in CNM. People in CNM relationships also focus particularly on the non-central role of sex within these relationships, which might challenge assumptions about sexuality in these relationships in clinical and research settings