A secretome profile indicative of oleate-induced proliferation of HepG2 hepatocellular carcinoma cells

Increased fatty acid (FA) is often observed in highly proliferative tumors. FAs have been shown to modulate the secretion of proteins from tumor cells, contributing to tumor survival. However, the secreted factors affected by FA have not been systematically explored. Here, we found that treatment of oleate, a monounsaturated omega-9 FA, promoted the proliferation of HepG2 cells. To examine the secreted factors associated with oleate-induced cell proliferation, we performed a comprehensive secretome profiling of oleate-treated and untreated HepG2 cells. A comparison of the secretomes identified 349 differentially secreted proteins (DSPs; 145 upregulated and 192 downregulated) in oleate-treated samples, compared to untreated samples. The functional enrichment and network analyses of the DSPs revealed that the 145 upregulated secreted proteins by oleate treatment were mainly associated with cell proliferation-related processes, such as lipid metabolism, inflammatory response, and ER stress. Based on the network models of the DSPs, we selected six DSPs (MIF, THBS1, PDIA3, APOA1, FASN, and EEF2) that can represent such processes related to cell proliferation. Thus, our results provided a secretome profile indicative of an oleate-induced proliferation of HepG2 cell

Vampires in the village Žrnovo on the island of Korčula: following an archival document from the 18th century

Središnja tema rada usmjerena je na raščlambu spisa pohranjenog u Državnom arhivu u Mlecima (fond: Capi del Consiglio de’ Dieci: Lettere di Rettori e di altre cariche) koji se odnosi na događaj iz 1748. godine u korčulanskom selu Žrnovo, kada su mještani – vjerujući da su se pojavili vampiri – oskvrnuli nekoliko mjesnih grobova. U radu se podrobno iznose osnovni podaci iz spisa te rečeni događaj analizira u širem društvenom kontekstu i prate se lokalna vjerovanja.The main interest of this essay is the analysis of the document from the State Archive in Venice (file: Capi del Consiglio de’ Dieci: Lettere di Rettori e di altre cariche) which is connected with the episode from 1748 when the inhabitants of the village Žrnove on the island of Korčula in Croatia opened tombs on the local cemetery in the fear of the vampires treating. This essay try to show some social circumstances connected with this event as well as a local vernacular tradition concerning superstitions

in: Damage Control Resuscitation

Airway care of the patient with life threatening haemorrhage presents many challenges during damage control resuscitation. The essential requirements are to maintain oxygenation at all stages of care and when necessary deliver general anaesthesia to facilitate invasive haemorrhage control procedures. In the remote, pre-hospital setting, providers must be able to assess the airway and intervene with a range of strategies to prevent hypoxaemia. These interventions may vary from basic airway opening manoeuvres to advanced techniques such as drug-assisted rapid sequence intubation. The initial delivery of these skills in remote settings will be the responsibility of whichever medical provider is present, and so their training, equipment and decision-making skills must reflect the challenges they will face. Rapid sequence intubation skills may not be widely available in remote environments and so providers must be equipped with alternative airway management strategies including cricothyrotomy and use of extraglottic airway devices. When invasive haemorrhage control procedures are required for patients with life threatening haemorrhage, rapid sequence intubation will need to be performed. This procedure carries significant risk in the presence of haemorrhagic shock. Providers must be aware of the hypotensive effects of induction agents and the adverse impact of positive pressure ventilation upon cardiac output in the presence of life threatening haemorrhage. The risks of intubation should be minimised with appropriate blood production administration and ventilation techniques as part of a coordinated damage control resuscitation strategy