17 research outputs found
Transcriptomic data from panarthropods shed new light on the evolution of insulator binding proteins in insects
Comparison of Intravitreal Bevacizumab Upload Followed by a Dexamethasone Implant versus Dexamethasone Implant Monotherapy for Retinal Vein Occlusion with Macular Edema
Early Results of Dexamethasone Implant, Ranibizumab, and Triamcinolone in Macular Edema Due to Branch Retinal Vein Occlusion
Twelve-month experience with Ozurdex for the treatment of macular edema associated with retinal vein occlusion
Photodynamic Therapy Versus Combination Therapy in Polypoidal Choroidal Vasculopathy: Changes of Aqueous Vascular Endothelial Growth Factor
Graphene coated with alumina and its utilization as a thermal conductivity enhancer for alumina sphere/thermoplastic polyurethane composite
The Effect of cilostazolon Glucose Tolerance and Insulin Resistance in a Rat Model of Non - insulin Dependent Diabetes Mellitus
Expansion of Chromosome Territories with Chromatin Decompaction in BAF53-depleted Interphase Cells
Chromosomes are compartmentalized into discrete chromosome territories during interphase in mammalian cells. A chromosome territory is generated by the tendency of chromatin to occupy the smallest shell volume, which is determined by the polymeric properties and interactions of the internal meshwork of the chromatin fiber. Here, we show that BAF53 knockdown by small interfering RNA interference led to the expansion of chromosome territories. This was accompanied by a reduction in chromatin compaction, an increase in the micrococcal nuclease sensitivity of the chromatin, and an alteration in H3-K9 and H3-K79 dimethylation. Interestingly, the BAF53 knockdown cells suffer a cell cycle defect. Despite the significant irregularity and decompaction of the polynucleosomes isolated from the BAF53 knockdown cells, the chromatin loading of H1 and core histones remained unaltered, as did the nucleosome spacing. The histone hyperacetylation and down-regulation of BRG-1, mBrm, and Tip49, the catalytic components of the SWI/SNF complex and the TIP60 complex, respectively, did not expand chromosome territories. These results indicate that BAF53 contributes to the polymeric properties and/or the internal meshwork interactions of the chromatin fiber probably via a novel mechanism