Accuracy-awareness: A pessimistic approach to optimal control of triggered mobile communication networks

We use nonlinear model predictive control to procure a joint control of mobility and transmission to minimize total network communication energy use. The nonlinear optimization problem is solved numerically in a self-triggered framework, where the next control update time depends on the predicted state trajectory and the accuracy of the numerical solution. Solution accuracy must be accounted for in any circumstance where systems are run in open-loop for long stretches of time based on potentially inaccurate predictions. These triggering conditions allow us to place wireless nodes in low energy ‘idle' states for extended periods, saving over 70% of energy compared to a periodic policy where nodes consistently use energy to receive control updates

Innovation and opportunity: review of the UK’s national AI strategy

The publication of the UK’s National Artificial Intelligence (AI) Strategy represents a step-change in the national industrial, policy, regulatory, and geo-strategic agenda. Although there is a multiplicity of threads to explore this text can be read primarily as a ‘signalling’ document. Indeed, we read the National AI Strategy as a vision for innovation and opportunity, underpinned by a trust framework that has innovation and opportunity at the forefront. We provide an overview of the structure of the document and offer an emphasised commentary on various standouts. Our main takeaways are: Innovation First: a clear signal is that innovation is at the forefront of UK’s data priorities. Alternative Ecosystem of Trust: the UK’s regulatory-market norms becoming the preferred ecosystem is dependent upon the regulatory system and delivery frameworks required. Defence, Security and Risk: security and risk are discussed in terms of utilisation of AI and governance. Revision of Data Protection: the signal is that the UK is indeed seeking to position itself as less stringent regarding data protection and necessary documentation. EU Disalignment—Atlanticism?: questions are raised regarding a step back in terms of data protection rights. We conclude with further notes on data flow continuity, the feasibility of a sector approach to regulation, legal liability, and the lack of a method of engagement for stakeholders. Whilst the strategy sends important signals for innovation, achieving ethical innovation is a harder challenge and will require a carefully evolved framework built with appropriate expertise

Is Blood Donation an Opportunity for Hypertension Awareness?

Blood centers serve as a cornerstone of public health by providing potentially lifesaving blood products. Interactions with millions of potential donors provides these centers with a unique means of health education and screening opportunities.https://scholarworks.uvm.edu/comphp_gallery/1081/thumbnail.jp

Preparation of multiplexed small RNA libraries from plants

[EN] High-throughput sequencing is a powerful tool for exploring small RNA populations in plants. The ever-increasing output from an Illumina Sequencing System allows for multiplexing multiple samples while still obtaining sufficient data for small RNA discovery and characterization. Here we describe a protocol for generating multiplexed small RNA libraries for sequencing up to 12 samples in one lane of an Illumina HiSeq System single-end, 50 base pair run. RNA ligases are used to add the 3¿ and 5¿ adaptors to purified small RNAs; ligation products that lack a small RNA molecule (adaptor-adaptor products) are intentionally depleted. After cDNA synthesis, a linear PCR step amplifies the DNA fragments. The 3¿ PCR primers used here include unique 6- nucleotide sequences to allow for multiplexing up to 12 samples.The original version of this protocol was described in Carbonell et al. (2012). The updated version of the protocol was described in Carbonell et al. (2014). This work was supported by grants from the National Science Foundation (MCB-0956526, MCB-1231726) and National Institutes of Health (AI043288)Gilbert, KB.; Fahlgren, N.; Kasschau, KD.; Chapman, EJ.; Carrington, JC.; Carbonell, A. (2014). Preparation of multiplexed small RNA libraries from plants. Bio-protocol. 4(21):1-17. https://doi.org/10.21769/BioProtoc.1275S11742

Opportunistic pathology-based screening for diabetes

OBJECTIVE To determine the potential of opportunistic glycated haemoglobin (HbA1c) testing of pathology samples to detect previously unknown diabetes. DESIGN Pathology samples from participants collected for other reasons and suitable for HbA1c testing were utilised for opportunistic diabetes screening. HbA1c was measured with a Biorad Variant II turbo analyser and HbA1c levels of ≥6.5% (48 mmol/mol) were considered diagnostic for diabetes. Confirmation of previously unknown diabetes status was obtained by a review of hospital medical records and phone calls to general practitioners. SETTING Hospital pathology laboratory receiving samples from hospital-based and community-based (CB) settings. PARTICIPANTS Participants were identified based on the blood sample collection location in the CB, emergency department (ED) and inpatient (IP) groups. Exclusions pretesting were made based on the electronic patient history of: age <18 years, previous diabetes diagnosis, query for diabetes status in the past 12 months, evidence of pregnancy and sample collected postsurgery or transfusion. Only one sample per individual participant was tested. RESULTS Of the 22 396 blood samples collected, 4505 (1142 CB, 1113 ED, 2250 IP) were tested of which 327 (7.3%) had HbA1c levels ≥6.5% (48 mmol/mol). Of these 120 (2.7%) were determined to have previously unknown diabetes (11 (1%) CB, 21 (1.9%) ED, 88 (3.9%) IP). The prevalence of previously unknown diabetes was substantially higher (5.4%) in hospital-based (ED and IP) participants aged over 54 years. CONCLUSIONS Opportunistic testing of referred pathology samples can be an effective method of screening for diabetes, especially in hospital-based and older persons.This project was supported by a grant from the Canberra Hospital Private Practice Trust Fund

A rare intercomparison of nutrient analysis at sea: lessons learned and recommendations to enhance comparability of open-ocean nutrient data

An intercomparison study has been carried out on the analysis of inorganic nutrients at sea following the operation of two nutrient analysers simultaneously on the GO-SHIP A02 trans-Atlantic survey in May 2017. Both instruments were Skalar San++ Continuous Flow Analyzers, one from the Marine Institute, Ireland and the other from Dalhousie University, Canada, each operated by their own laboratory analysts following GO-SHIP guidelines while adopting their existing laboratory methods. There was high comparability between the two data sets and vertical profiles of nutrients also compared well with those collected in 1997 along the same A02 transect by the World Ocean Circulation Experiment. The largest differences between data sets were observed in the low-nutrient surface waters and results highlight the value of using three reference materials (low, middle and high concentration) to cover the full range of expected nutrients and identify bias and non-linearity in the calibrations. The intercomparison also raised some interesting questions on the comparison of nutrients analysed by different systems and a number of recommendations have been suggested that we feel will enhance the existing GO-SHIP guidelines to improve the comparability of global nutrient data sets. A key recommendation is for the specification of clearly defined data quality objectives for oceanic nutrient measurements and a flagging method for reported data that do not meet these criteria. The A02 nutrient data set is currently available at the National Oceanographic Data Centre of Ireland: https://doi.org/10.20393/CE49BC4C-91CC-41B9-A07F-D4E36B18B26F and https://doi.org/10.20393/EAD02A1F-AAB3-4F4E-AD60-6289B9585531.</p

Xpert MTB/RIF Assay Shows Faster Clearance of Mycobacterium tuberculosis DNA with Higher Levels of Rifapentine Exposure.

The Xpert MTB/RIF assay is both sensitive and specific as a diagnostic test. Xpert also reports quantitative output in cycle threshold (CT) values, which may provide a dynamic measure of sputum bacillary burden when used longitudinally. We evaluated the relationship between Xpert CT trajectory and drug exposure during tuberculosis (TB) treatment to assess the potential utility of Xpert CT for treatment monitoring. We obtained serial sputum samples from patients with smear-positive pulmonary TB who were consecutively enrolled at 10 international clinical trial sites participating in study 29X, a CDC-sponsored Tuberculosis Trials Consortium study evaluating the tolerability, safety, and antimicrobial activity of rifapentine at daily doses of up to 20 mg/kg of body weight. Xpert was performed at weeks 0, 2, 4, 6, 8, and 12. Longitudinal CT data were modeled using a nonlinear mixed effects model in relation to rifapentine exposure (area under the concentration-time curve [AUC]). The rate of change of CT was higher in subjects receiving rifapentine than in subjects receiving standard-dose rifampin. Moreover, rifapentine exposure, but not assigned dose, was significantly associated with rate of change in CT (P = 0.02). The estimated increase in CT slope for every additional 100 μg · h/ml of rifapentine drug exposure (as measured by AUC) was 0.11 CT/week (95% confidence interval [CI], 0.05 to 0.17). Increasing rifapentine exposure is associated with a higher rate of change of Xpert CT, indicating faster clearance of Mycobacterium tuberculosis DNA. These data suggest that the quantitative outputs of the Xpert MTB/RIF assay may be useful as a dynamic measure of TB treatment response

Comparing typologies of violence exposure and associations with syndemic health outcomes among cisgender and transgender female sex workers living with HIV in the Dominican Republic

Violence against women research largely excludes transgender women’s experiences and violence from perpetrators other than intimate partners. This study compares patterns of violence exposure among cisgender and transgender female sex workers (FSWs) and the associations with syndemic health outcomes. We used cross-sectional surveys from samples of cisgender and transgender FSWs living with HIV in the Dominican Republic (N = 211 and 100, respectively). We used latent class analysis to identify patterns of emotional, physical and sexual violence and harassment by partners, clients, and police. We assessed sociodemographic and occupational predictors in relation to class membership, and class membership in relation to health (HIV continuum of care outcomes, mental health, substance use), using logistic regression. Two classes were identified in cisgender sample: Low Reported Violence Exposure (Class 1) and Sex Work-related Police Harassment (Class 2). Class 2 participants had greater odds of scoring abnormal or borderline abnormal anxiety on the Hospital Anxiety and Depression Scale (HADS-A) (adjusted OR = 3.97 p<0.01), moderate-to-severe depression per the Patient Health Questionnaire-9 (PHQ-9) (aOR = 5.74, p<0.01), and any illicit drug use in the past six months (aOR = 3.06, p<0.05) compared to Class 1. The transgender sample produced three classes: Low Reported Violence Exposure (Class 1); Sex Work-related Police Harassment (Class 2); and Sex Workrelated Violence and Harassment (Class 3). Class 3 participants had greater odds of having anxiety (aOR = 6.65, p<0.01) and depression (aOR = 4.45, p<0.05), while Class 2 participants had greater odds of perfect ART adherence during the previous four days (aOR = 2.78, p<0.05), compared to Class 1. The more diverse and extreme violence patterns uncovered for the transgender sample show this group’s heightened risk, while similar patterns across groups regarding police abuse highlight a need for police-focused violence prevention interventions. Each sample’s highest violence class was associated with poor mental health, underscoring the need for mental health interventions for all FSWs