Low energy availability assessed by a sport-specific questionnaire and clinical interview indicative of bone health, endocrine profile and cycling performance in competitive male cyclists

Objectives To evaluate the efficacy of a sport-specific energy availability (EA) questionnaire, combined with clinical interview, for identifying male athletes at risk of developing bone health, endocrine and performance consequences of relative energy deficiency in sports (RED-S). Methods Fifty competitive male road cyclists, recruited through links of participants in a pilot study, were assessed by a newly developed sport-specific questionnaire and clinical interview (SEAQ-I) and received dual energy X-ray absorptiometry (DXA) bone mineral density (BMD) and body composition scans and blood tests for endocrine markers. Results Low EA as assessed using the SEAQ-I, was observed in 28% of cyclists. Low lumbar spine BMD (Z-score<−1.0) was found in 44% of cyclists. EA was the most significant determinant of lumbar spine BMD Z-score (p<0.001). Among low EA cyclists, lack of previous load-bearing sport was associated with the lowest BMD (p=0.013). Low EA was associated with reduced total percentage fat (p<0.019). The 10 cyclists with chronic low EA had lower levels of testosterone compared with those having adequate EA (p=0.024). Mean vitamin D concentration was below the level recommended for athletes (90  nmol/L). Training loads were positively associated with power-to-weight ratios, assessed as 60  min functional threshold power (FTP) per kg (p<0.001). Percentage body fat was not significantly linked to cycling performance. Conclusions This study demonstrates that a SEAQ-I is effective for identifying male road cyclists with acute intermittent and chronic sustained low EA. Cyclists with low EA, particularly in the long-term, displayed adverse quantifiable measures of bone, endocrinology and performance consequences of RED-S

Management options for pregnant feedlot heifers

Heifers that were 167 days pregnant when slaughtered gained faster and more efficiently than open heifers, or heifers that had been aborted with a prostaglandin analog at 83 or 138 days, unless the slaughter weight was adjusted for the 1.7% lower carcass yield (dressing %). When the slaughter weights for all these management options were adjusted using the carcass yield of open heifers, there was no difference in gain except for the depressed performance associated with late abortions. However, open heifers were 6.7% more efficient than heifers pregnant when slaughtered. Heifers aborted at 138 days had substantially reduced gains and feed conversion. These results indicate that because of increased carcass yield, packers can afford to pay a premium for heifers that are open or have been aborted during the first trimester. Unless a premium is paid for open heifers, pregnant heifers (provided they are sold before calving) sold on a live weight basis might be more profitable because of the apparent increased gain and efficiency

Calcutta Botanic Garden and the colonial re-ordering of the Indian environment

This article examines three hand-painted colour maps that accompanied the annual report of the Calcutta Botanic Garden for 1846 to illustrate how the Garden’s layout, uses and functions had changed over the previous 30 years. The evolution of the Calcutta Botanic Garden in the first half of the nineteenth-century reflects a wider shift in attitudes regarding the relationship between science, empire and the natural world. On a more human level the maps result from, and illustrate, the development of a vicious personal feud between the two eminent colonial botanists charged with superintending the garden in the 1840s

The Tsaatan Reindeer Herders of Mongolia : Forgotten lessons of of human-animal systems

For millennia, humans have depended on animals for resources necessary for survival. The Tsaatan people of northern Mongolia are a nomadic people who depend on reindeer for nearly all aspects of survival, as well as cultural, spiritual, linguistic, and economic identity

Assessing the Anti-Inflammatory Activity of the Anxiolytic Drug Buspirone Using CRISPR-Cas9 Gene Editing in LPS-Stimulated BV-2 Microglial Cells

Buspirone is an anxiolytic drug with robust serotonin receptor 1A (Htr1a) agonist activities. However, evidence has demonstrated that this drug also targets the dopamine D3 receptor (Drd3), where it acts as a potent antagonist. In vivo, Drd3 blockade is neuroprotective and reduces inflammation in models of Parkinson's disease. To test if buspirone also elicited anti-inflammatory activities in vitro, we generated stable Drd3-/- and Htr1a-/- BV2 microglial cell lines using CRISPR-Cas9 technology and then tested the effects of buspirone after lipopolysaccharide (LPS) challenge. We found that LPS exposure had no effect on cell viability, except in Htr1a-/- cells, where viability was reduced (p -/- cells, but not in WT or Htr1a-/- cells. Buspirone counteracted LPS-induced NO release, NOS2, IL-1β and TNF-α gene expression in WT cells, whereas it exerted limited effects in Drd3-/- or Htr1a-/- microglia. In summary, our findings indicate that buspirone attenuates microglial polarization after LPS challenge. These results also highlight some major effects of Drd3 or Htr1a genetic ablation on microglial biology, raising important questions on the complex role of neurotransmitters in regulating microglia functions

Theory of Coherent Time-dependent Transport in One-dimensional Multiband Semiconductor Superlattices

We present an analytical study of one-dimensional semiconductor superlattices in external electric fields, which may be time-dependent. A number of general results for the (quasi)energies and eigenstates are derived. An equation of motion for the density matrix is obtained for a two-band model, and the properties of the solutions are analyzed. An expression for the current is obtained. Finally, Zener-tunneling in a two-band tight-binding model is considered. The present work gives the background and an extension of the theoretical framework underlying our recent Letter [J. Rotvig {\it et al.}, Phys. Rev. Lett. {\bf 74}, 1831 (1995)], where a set of numerical simulations were presented.Comment: 15 pages, Revtex 3.0, uses epsf, 2 ps figures attache

Metformin Treatment Attenuates Brain Inflammation and Rescues PACAP/VIP Neuropeptide Alterations in Mice Fed a High-Fat Diet.

High-fat diet (HFD)-induced comorbid cognitive and behavioural impairments are thought to be the result of persistent low-grade neuroinflammation. Metformin, a first-line medication for the treatment of type-2 diabetes, seems to ameliorate these comorbidities, but the underlying mechanism(s) are not clear. Pituitary adenylate cyclase-activating peptide (PACAP) and vasoactive intestinal peptide (VIP) are neuroprotective peptides endowed with anti-inflammatory properties. Alterations to the PACAP/VIP system could be pivotal during the development of HFD-induced neuroinflammation. To unveil the pathogenic mechanisms underlying HFD-induced neuroinflammation and assess metformin's therapeutic activities, (1) we determined if HFD-induced proinflammatory activity was present in vulnerable brain regions associated with the development of comorbid behaviors, (2) investigated if the PACAP/VIP system is altered by HFD, and (3) assessed if metformin rescues such diet-induced neurochemical alterations. C57BL/6J male mice were divided into two groups to receive either standard chow (SC) or HFD for 16 weeks. A further HFD group received metformin (HFD + M) (300 mg/kg BW daily for 5 weeks) via oral gavage. Body weight, fasting glucose, and insulin levels were measured. After 16 weeks, the proinflammatory profile, glial activation markers, and changes within the PI3K/AKT intracellular pathway and the PACAP/VIP system were evaluated by real-time qPCR and/or Western blot in the hypothalamus, hippocampus, prefrontal cortex, and amygdala. Our data showed that HFD causes widespread low-grade neuroinflammation and gliosis, with regional-specific differences across brain regions. HFD also diminished phospho-AKT(Ser473) expression and caused significant disruptions to the PACAP/VIP system. Treatment with metformin attenuated these neuroinflammatory signatures and reversed PI3K/AKT and PACAP/VIP alterations caused by HFD. Altogether, our findings demonstrate that metformin treatment rescues HFD-induced neuroinflammation in vulnerable brain regions, most likely by a mechanism involving the reinstatement of PACAP/VIP system homeostasis. Data also suggests that the PI3K/AKT pathway, at least in part, mediates some of metformin's beneficial effects

S1×S2S^1 \times S^2 wormholes and topological charge

I investigate solutions to the Euclidean Einstein-matter field equations with topology $S^1 \times S^2 \times R$ in a theory with a massless periodic scalar field and electromagnetism. These solutions carry winding number of the periodic scalar as well as magnetic flux. They induce violations of a quasi-topological conservation law which conserves the product of magnetic flux and winding number on the background spacetime. I extend these solutions to a model with stable loops of superconducting cosmic string, and interpret them as contributing to the decay of such loops.Comment: 18 pages (includes 6 figs.), harvmac and epsf, CU-TP-62

Time Periodic Behavior of Multiband Superlattices in Static Electric Fields

We use an analytic perturbation expansion for the two-band system of tight binding electrons to discuss Bloch oscillations and Zener tunneling within this model. We make comparison with recent numerical results and predict analytically the frequency of radiation expected from Zener tunneling, including its disappearance, as a function of the system parameters.Comment: 12 pages, no figure include