Transcriptional regulation of the human ALDH1A1 promoter by the oncogenic homeoprotein TLX1/HOX11

The homeoprotein TLX1, which is essential to spleen organogenesis and oncogenic when aberrantly expressed in immature T cells, functions as a bifunctional transcriptional regulator, being capable of activation or repression depending on cell type and/or promoter context. However, the detailed mechanisms by which it regulates the transcription of target genes such as ALDH1A1 remains to be elucidated. We therefore functionally assessed the ability of TLX1 to regulate ALDH1A1 expression in two hematopoietic cell lines, PER-117 T-leukemic cells and human erythroleukemic (HEL) cells, by use of luciferase reporter and mobility shift assays. We showed that TLX1 physically interacts with the general transcription factor TFIIB via its homeodomain, and identified two activities in respect to TLX1-mediated regulation of the CCAAT box-containing ALDH1A1 promoter. The first involved CCAAT-dependent transcriptional repression via perturbation of GATA factor-containing protein complexes assembled at a non-canonical TATA (GATA) box. A structurally intact homeodomain was essential for repression by TLX1 although direct DNA binding was not required. The second activity, which involved CCAAT-independent transcriptional activation did not require an intact homeodomain, indicating that the activation and repression functions of TLX1 are distinct. These findings confirm ALDH1A1 gene regulation by TLX1 and support an indirect model for TLX1 function, in which protein-protein interactions, rather than DNA binding at specific sites, are crucial for its transcriptional activity

Identification of genes associated with blood feeding in the cat flea, Ctenocephalides felis

Background: The cat flea (Ctenocephalides felis) is a blood-feeding ectoparasitic insect and particular nuisance pest of companion animals worldwide. Identification of genes that are differentially expressed in response to feeding is important for understanding flea biology and discovering targets for their control. Methods: C. felis fleas were maintained and fed for 24 h using an artificial rearing system. The technique of suppression subtractive hybridization was employed to screen for mRNAs specifically expressed in fed fleas. Results: We characterized nine distinct full-length flea transcripts that exhibited modulated or de novo expression during feeding. Among the predicted protein sequences were two serine proteases, a serine protease inhibitor, two mucin-like molecules, a DNA topoisomerase, an enzyme associated with GPI-mediated cell membrane attachment of proteins and a component of the insect innate immune response. Conclusions: Our results provide a molecular insight into the physiology of flea feeding. The protein products of the genes identified may play important roles during flea feeding in terms of blood meal digestion, cellular growth/repair and protection from feeding-associated stresses

Spinflation from Geometric Tachyon

We study the assisted inflation scenario from the rolling of $N$ BPS D3-brane into the NS5-branes, on a transverse geometry of $R^3 \times S^1$, coupled to four dimensional gravity. We assume that the branes are distributed along $S^1$ and the probe D3-branes spin along $R^3$ plane. Qualitatively this process is similar to that of N-tachyon assisted inflation on unstable D-branes. We further study the spinflation scenario numerically and analyze its effect.Comment: 18pages, 9 figures, added clarifications, to appear in JHE

β -decay measurements of 12B with Gammasphere

The β-decay branching ratio of 12B to the Hoyle state in 12C was measured by detection of γ rays. 12B nuclei were produced via the 11B(d,p)12B reaction in inverse kinematics on a TiD2 target. The present results corroborate those obtained recently for the β branch by implantation. The value from both experiments is inconsistent with that accepted in the literature

The cosmic ultraviolet baryon survey (CUBS): IV: The complex multiphase circumgalactic medium as revealed by partial Lyman limit systems

Galaxie

The cosmic ultraviolet baryon survey (CUBS): III. Physical properties and elemental abundances of Lyman-limit systems at z < 1

Galaxie

The cosmic ultraviolet baryon survey (CUBS): II. Discovery of an H2_2 -bearing DLA in the vicinity of an early-type galaxy at z = 0.576

Interstellar matter and star formatio

The Cosmic Ultraviolet Baryon Survey (CUBS) - I. Overview and the diverse environments of Lyman limit systems at z < 1

Galaxie