27 research outputs found

    Reply to: Validity of managing peatlands with fire

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    Replying to: Baird, A.J. et al. Validity of managing peatlands with fire. Nature Geoscience 12(11), 884–885 (2019) doi:10.1038/s41561-019-0477-

    Characterization of IS1110, a highly mobile genetic element from Mycobacterium avium

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    [EN] A highly mobile insertion sequence designated IS1110 was detected in Mycobacterium avium strain LR541 following an observed increase in size of the plasmid pLR20. Genomic libraries of M. avium strains carrying either parental pLR20 or the modified plasmid (pLR20') were constructed and the sequence of the relevant clones was determined to characterize the insertion sequence and the target region. IS1110 Is a 1457bp element lacking terminal inverted repeats, and is related to IS900 (from Mycobacterium paratuberculosis), IS901 and IS902 (from M. avium) and to IS116 (from Streptomyces clavuligerus). LR541 carries several copies of IS1110. Individual colonies from the same plate show differences in Southern blot patterns when tested with an IS1110-derived probe; the ability to detect transposition events in random colonies, without any selection pressure, indicates an exceptionally high degree of mobility, which will be invaluable for transposon mutagenesis. Analyses of M. avium isolates from human, veterinary, and environmental sources showed that IS1110-hybridizing sequences are present in some M. avium isolates but they were not detected in strains of other mycobacterial species. The polymorphism exhibited in M. avium isolates suggests that this element may be useful for molecular epidemiological studies f M. avium infections.We are grateful for support from the Medical Research Council (Grant No. SPG 8825658), the Spanish Ministry of Education and Science (Grant No. PG90 52552468), and the Commission of the European Communities. Our thanks are due to Jack Crawford for provision of strain LR541, and to JohnJoe McFadden and Tim Doran for helpful discussions. This work benefitted from the use of the SERC SEQNET facility at Daresbury.Hernández Pérez, M.; Fomukong, N.; Hellyer, T.; Brown, I.; Dale, J. (1994). Characterization of IS1110, a highly mobile genetic element from Mycobacterium avium. 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Identification, cloning, and sequencing of piv, a new gene involved in inverting the pilin genes of Moraxella lacunata. Journal of Bacteriology, 172(8), 4370-4377. doi:10.1128/jb.172.8.4370-4377.1990Meissner, P. S., & Falkinham, J. O. (1986). Plasmid DNA Profiles as Epidemiological Markers for Clinical and Environmental Isolates of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum. Journal of Infectious Diseases, 153(2), 325-331. doi:10.1093/infdis/153.2.325Moss, M. T., Malik, Z. P., Tizard, M. L. V., Green, E. P., Sanderson, J. D., & Hermon-Taylor, J. (1992). IS902, an insertion element of the chronic-enteritis-causing Mycobacterium avium subsp. silvaticum. Journal of General Microbiology, 138(1), 139-145. doi:10.1099/00221287-138-1-139Murray, A., Winter, N., Lagranderie, M., Hill, D. F., Rauzier, J., Timm, J., … Gicquel, B. (1992). 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