Rapid enzymatic test for phenotypic HIV protease drug resistance

A phenotypic resistance test based on recombinant expression of the active HIV protease in E. coli from patient blood samples was developed. The protease is purified in a rapid onestep procedure as active enzyme and tested for inhibition by five selected synthetic inhibitors (amprenavir, indinavir, nelfinavir, ritonavir, and saquinavir) used presently for chemotherapy of HIVinfected patients. The HPLC system used in a previous approach was replaced by a continuous fluorogenic assay suitable for highthroughput screening on microtiter plates. This reduces significantly the total assay time and allows the determination of inhibition constants (K-i). The Michaelis constant (K-m) and the inhibition constant (K-i) of recombinant wildtype protease agree well with published data for cloned HIV protease. The enzymatic test was evaluated with recombinant HIV protease derived from eight HIVpositive patients scored from sensitive to highly resistant according to mutations detected by genotypic analysis. The measured K-i values correlate well with the genotypic resistance scores, but allow a higher degree of differentiation. The noninfectious assay enables a more rapid yet sensitive detection of HIV protease resistance than other phenotypic assays

Probing the extreme realm of AGN feedback in the massive galaxy cluster, RX J1532.9+3021

We present a detailed Chandra, XMM-Newton, VLA and HST analysis of one of the strongest cool core clusters known, RX J1532.9+3021 (z=0.3613). Using new, deep 90 ks Chandra observations, we confirm the presence of a western X-ray cavity or bubble, and report on a newly discovered eastern X-ray cavity. The total mechanical power associated with these AGN-driven outflows is (22+/-9)*10^44 erg/s, and is sufficient to offset the cooling, indicating that AGN feedback still provides a viable solution to the cooling flow problem even in the strongest cool core clusters. Based on the distribution of the optical filaments, as well as a jet-like structure seen in the 325 MHz VLA radio map, we suggest that the cluster harbours older outflows along the north to south direction. The jet of the central AGN is therefore either precessing or sloshing-induced motions have caused the outflows to change directions. There are also hints of an X-ray depression to the north aligned with the 325 MHz jet-like structure, which might represent the highest redshift ghost cavity discovered to date. We further find evidence of a cold front (r=65kpc) that coincides with the outermost edge of the western X-ray cavity and the edge of the radio mini-halo. The common location of the cold front with the edge of the radio mini-halo supports the idea that the latter originates from electrons being reaccelerated due to sloshing induced turbulence. Alternatively, its coexistence with the edge of the X-ray cavity may be due to cool gas being dragged out by the outburst. We confirm that the central AGN is highly sub-Eddington and conclude that a >10^10M_Sun or a rapidly spinning black hole is favoured to explain both the radiative-inefficiency of the AGN and the powerful X-ray cavities.Comment: Accepted for publication to ApJ (minor corrections), 16 pages, 16 figures, 5 tables. Full resolution at http://www.stanford.edu/~juliehl/M1532

Quantum Games and Quantum Strategies

We investigate the quantization of non-zero sum games. For the particular case of the Prisoners' Dilemma we show that this game ceases to pose a dilemma if quantum strategies are allowed for. We also construct a particular quantum strategy which always gives reward if played against any classical strategy.Comment: 4 pages, 4 figures, typographic sign error in the definition of the operator J correcte

Spectral modeling of gaseous metal disks around DAZ white dwarfs

We report on our attempt for the first non-LTE modeling of gaseous metal disks around single DAZ white dwarfs recently discovered by Gaensicke et al. and thought to originate from a disrupted asteroid. We assume a Keplerian rotating viscous disk ring composed of calcium and hydrogen and compute the detailed vertical structure and emergent spectrum. We find that the observed infrared CaII emission triplet can be modeled with a hydrogen-deficient gas ring located at R=1.2 R_sun, inside of the tidal disruption radius, with Teff about 6000 K and a low surface mass density of about 0.3 g/cm**2. A disk having this density and reaching from the central white dwarf out to R=1.2 R_sun would have a total mass of 7 10**21 g, corresponding to an asteroid with about 160 km diameter.Comment: Proceedings, 16th European White Dwarf Workshop, Barcelona, 200

Quadrupole shape invariants in the interacting boson model

In terms of the Interacting Boson Model, shape invariants for the ground state, formed by quadrupole moments up to sixth order, are studied in the dynamical symmetry limits and, for the first time, over the whole structural range of the IBM-1. The results are related to the effective deformation parameters and their fluctuations in the geometrical model. New signatures that can distinguish vibrator and gamma-soft rotor structures, and one that is related to shape coexistence, are identified.Comment: 10 pages, ReVTeX, epsf, 2 Postscript figures include

Strong fragmentation of low-energy electromagnetic excitation strength in 117^{117}Sn

Results of nuclear resonance fluorescence experiments on $^{117}$Sn are reported. More than 50 $\gamma$ transitions with $E_{\gamma} < 4$ MeV were detected indicating a strong fragmentation of the electromagnetic excitation strength. For the first time microscopic calculations making use of a complete configuration space for low-lying states are performed in heavy odd-mass spherical nuclei. The theoretical predictions are in good agreement with the data. It is concluded that although the E1 transitions are the strongest ones also M1 and E2 decays contribute substantially to the observed spectra. In contrast to the neighboring even $^{116-124}$Sn, in $^{117}$Sn the $1^-$ component of the two-phonon $[2^+_1 \otimes 3^-_1]$ quintuplet built on top of the 1/2$^+$ ground state is proved to be strongly fragmented.Comment: 4 pages, 3 figure

Osteosarcoma of the mobile spine†

Background The aims of this analysis were to investigate features and outcome of high-grade osteosarcomas of the mobile spine. Patients and methods Since 1977, 20 Cooperative Osteosarcoma Study Group patients had a diagnosis of high-grade osteosarcomas of the mobile spine and were included in this retrospective analysis of patient-, tumor- and treatment-related variables and outcome. Results The median age was 29 years (range 5-58). Most frequent tumor sites were thoracic and lumbar spine. All but three patients had nonmetastatic disease at diagnosis. Treatment included surgery and chemotherapy for all patients, 13 were also irradiated. Eight patients failed to achieve a macroscopically complete surgical remission (five local, one primary metastases, two both), six died, two are alive, both with radiotherapy. Of 12 patients with complete remission at all sites, three had a recurrence (two local, one metastases) and died. The median follow-up of the 11 survivors was 8.7 years (range 3.1-22.3), 5-year overall and event-free survival rates were 60% and 43%. Age <40 years, nonmetastatic disease at diagnosis and complete remission predicted for better overall survival (OS, P < 0.05). Conclusions Osteosarcomas of the mobile spine are rare. With complete resection (and potentially radiotherapy) and chemotherapy, prognosis may be comparable with that of appendicular osteosarcoma