Buckling of stiffened curved panels under uniform axial compression

In bridge construction, the use of stiffened plates for box-girder or steel beams is common day to day practice. The advantages of the stiffening from the economical and mechanical points of view are unanimously recognized. For curved steel panels, however, applications are more recent and the literature on their mechanical behaviour including the influence of stiffeners is therefore limited. Their design with commercial finite element software is significantly time-consuming, which reduces the number of parameters which can be investigated in an optimization procedure. The present paper is thus dedicated to the study of the behaviour of stiffened curved panels under uniform longitudinal compression. It addresses the linear buckling and the ultimate strength which are both influenced by the coupled effects of curvature and stiffening. It finally proposes a design methodology based on that for stiffened flat plates adopted by European Standards and a column-like behaviour

Shear correction factors for functionally graded plates

International audienceThe Reissner-Mindlin plate model for calculation of functionally graded materials has been proposed in literature by using shear correction coefficient of homogeneous model. However, this use is a priori not appropriate for the gradient material. Identification of the transverse shear factors is thus investigated in this paper. The transverse shear stresses are derived by using energy considerations from the expression of membrane stresses. Using the obtained transverse shear factor, a numerical analysis is performed on a simply supported FG square plate whose elastic properties are isotropic at each point and vary through the thickness according to a power law distribution. The numerical results of a static analysis are compared with available solutions from previous studies

Green's operator for a periodic medium with traction-free boundary conditions and computation of the effective properties of thin plates

International audienceThis paper presents a new Green's operator (Gamma-operator) for mixed periodic and stress-free boundary conditions. The solution is split into a classical periodic solution and a complementary solution to account for stress-free boundary conditions. This operator is used to obtain the effective properties of thin heterogeneous plates made of periodic cells, using the Fast Fourier Transform, the periodic thin plate theory and the computation of the strain energy within the cell. Numerical applications are provided, including comparisons with closed-form solutions and finite element solutions

First-order shear deformation plate models for functionally graded materials

International audienceIn this paper, first-order shear deformation plate models for modelling structures made of functionally graded materials are proposed. Identification of transverse shear factors is investigated through these models by energy equivalence. The transverse shear stresses are derived from the expression of membrane stresses and equilibrium equations. Using the obtained transverse shear factor, a numerical analysis is performed on the examples of a simply supported square plate and of a cylindrical bending sandwich plate clamped at both ends. The material properties are assumed to be isotropic at each point and vary through the thickness according to a power law distribution. The numerical results of the static analysis are compared to available solutions from previous studies

Mining Modification of River Systems: A case study from the Australian Gold Rush

Mobilisation of large volumes of bedrock, regolith and soil has long been a characteristic feature of metal mining. Prior to the 20th century this was most efficiently achieved through harnessing the motive power of water. Large-scale water use in mining produced waste sands, gravels and silts that were flushed downstream, triggering changes in stream and floodplain morphology and function. During the 19th century the shift from artisanal to industrialised mining resulted in a rapid increase in the scale and extent of environmental change. This paper presents results from a multi-disciplinary research program investigating the environmental effects of 19th-century gold mining on waterways in south-eastern Australia. Archaeological and geospatial landscape survey are combined with historical data modelling and geomorphological analysis to examine the extractive processes that produced sediment in headwater regions and how this influenced fluvial processes operating on downstream waterways and floodplains. Our case study of the Three Mile-Hodgson Creek system on the Ovens (Beechworth) goldfield in north-east Victoria indicates that miners mobilised up to 7.3 million m3 of sediment in this small catchment alone. Results of the research suggest that tailings dams and sludge channels in this catchment are important archaeological evidence for early attempts to manage industrial waste

Genome-Wide SNP Discovery and Mapping QTLs for Seed Iron and Zinc Concentrations in Chickpea (Cicer arietinum L.)

Biofortification through plant breeding is a cost-effective and sustainable approach towards addressing micronutrient malnutrition prevailing across the globe. Screening cultivars for micronutrient content and identification of quantitative trait loci (QTLs)/genes and markers help in the development of biofortified varieties in chickpea (Cicer arietinum L.). With the aim of identifying the genomic regions controlling seed Fe and Zn concentrations, the F2 : 3 population derived from a cross between MNK-1 and Annigeri 1 was genotyped using genotyping by sequencing approach and evaluated for Fe and Zn concentration. An intraspecific genetic linkage map comprising 839 single nucleotide polymorphisms (SNPs) spanning a total distance of 1,088.04 cM with an average marker density of 1.30 cM was constructed. By integrating the linkage map data with the phenotypic data of the F2 : 3 population, a total of 11 QTLs were detected for seed Fe concentration on CaLG03, CaLG04, and CaLG05, with phenotypic variation explained ranging from 7.2% (CaqFe3.4) to 13.4% (CaqFe4.2). For seed Zn concentration, eight QTLs were identified on CaLG04, CaLG05, and CaLG08. The QTLs individually explained phenotypic variations ranging between 5.7%(CaqZn8.1) and 13.7%(CaqZn4.3). Three QTLs for seed Fe and Zn concentrations (CaqFe4.4, CaqFe4.5, and CaqZn4.1) were colocated in the “QTL-hotspot” region on CaLG04 that harbors several drought tolerance-related QTLs. We identified genes in the QTL regions that encode iron–sulfur metabolism and zinc-dependent alcohol dehydrogenase activity on CaLG03, iron ion binding oxidoreductase on CaLG04, and zinc-induced facilitator-like protein and ZIP zinc/iron transport family protein on CaLG05. These genomic regions and the associated markers can be used in marker-assisted selection to increase seed Fe and Zn concentrations in agronomically superior chickpea varieties

Genome analysis and physiological comparison of Alicycliphilus denitrificans strains BC and K601T

The genomes of the Betaproteobacteria Alicycliphilus denitrificans strains BC and K601T have been sequenced to get insight into the physiology of the two strains. Strain BC degrades benzene with chlorate as electron acceptor. The cyclohexanol-degrading denitrifying strain K601T is not able to use chlorate as electron acceptor, while strain BC cannot degrade cyclohexanol. The 16S rRNA sequences of strains BC and K601T are identical and the fatty acid methyl ester patterns of the strains are similar. Basic Local Alignment Search Tool (BLAST) analysis of predicted open reading frames of both strains showed most hits with Acidovorax sp. JS42, a bacterium that degrades nitro-aromatics. The genomes include strain-specific plasmids (pAlide201 in strain K601T and pAlide01 and pAlide02 in strain BC). Key genes of chlorate reduction in strain BC were located on a 120 kb megaplasmid (pAlide01), which was absent in strain K601T. Genes involved in cyclohexanol degradation were only found in strain K601T. Benzene and toluene are degraded via oxygenase-mediated pathways in both strains. Genes involved in the meta-cleavage pathway of catechol are present in the genomes of both strains. Strain BC also contains all genes of the ortho-cleavage pathway. The large number of mono- and dioxygenase genes in the genomes suggests that the two strains have a broader substrate range than known thus far.This research was supported by the Technology Foundation, the Applied Science Division (STW) of the Netherlands Organization for Scientific Research (NWO), project number 08053, the graduate school WIMEK (Wageningen Institute for Environment and Climate Research, which is part of SENSE Research School for Socio-Economic and Natural Sciences of the Environment, www.wimek-new.wur.nl and www.sense.nl), SKB (Dutch Centre for Soil Quality Management and Knowledge Transfer, www.skbodem.nl) and the Consolider project CSD-2007-00055. The research was incorporated in the TRIAS (TRIpartite Approaches 469 toward Soil systems processes) program (http://www.nwo.nl/en/research-and-results/programmes/alw/trias-tripartite-approach-to-soil-system-processes/index. html). Flávia Talarico Saia was supported by a FAPESP (the State of São Paulo Research Foundation) scholarship (2006-01997/5). The work conducted by the DOE JGI is supported by the Office of Science of the United States Department of Energy under contract number DE-AC02-05CH11231. Alfons Stams acknowledges support by an ERC (European Research Counsil) advanced grant (project 323009). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

A benzene-degrading nitrate-reducing microbial consortium displays aerobic and anaerobic benzene degradation pathways

All sequence data from this study were deposited at the European Bioinformatics Institute under the accession numbers ERS1670018 to ERS1670023. Further, all assigned genes, taxonomy, function, sequences of contigs, genes and proteins can be found in Table S3.In this study, we report transcription of genes involved in aerobic and anaerobic benzene degradation pathways in a benzene-degrading denitrifying continuous culture. Transcripts associated with the family Peptococcaceae dominated all samples (2136% relative abundance) indicating their key role in the community. We found a highly transcribed gene cluster encoding a presumed anaerobic benzene carboxylase (AbcA and AbcD) and a benzoate-coenzyme A ligase (BzlA). Predicted gene products showed >96% amino acid identity and similar gene order to the corresponding benzene degradation gene cluster described previously, providing further evidence for anaerobic benzene activation via carboxylation. For subsequent benzoyl-CoA dearomatization, bam-like genes analogous to the ones found in other strict anaerobes were transcribed, whereas gene transcripts involved in downstream benzoyl-CoA degradation were mostly analogous to the ones described in facultative anaerobes. The concurrent transcription of genes encoding enzymes involved in oxygenase-mediated aerobic benzene degradation suggested oxygen presence in the culture, possibly formed via a recently identified nitric oxide dismutase (Nod). Although we were unable to detect transcription of Nod-encoding genes, addition of nitrite and formate to the continuous culture showed indication for oxygen production. Such an oxygen production would enable aerobic microbes to thrive in oxygen-depleted and nitrate-containing subsurface environments contaminated with hydrocarbons.This study was supported by a grant of BE-Basic-FES funds from the Dutch Ministry of Economic Affairs. The research of A.J.M. Stams is supported by an ERC grant (project 323009) and the gravitation grant “Microbes for Health and Environment” (project 024.002.002) of the Netherlands Ministry of Education, Culture and Science. F. Hugenholtz was supported by the same gravitation grant (project 024.002.002). B. Hornung is supported by Wageningen University and the Wageningen Institute for Environment and Climate Research (WIMEK) through the IP/OP program Systems Biology (project KB-17-003.02-023).info:eu-repo/semantics/publishedVersio

Regulatory network modelling of iron acquisition by a fungal pathogen in contact with epithelial cells

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