Localized modes at a D-brane--O-plane intersection and heterotic Alice strings

We study a system of $N_c$ $D3$-branes intersecting $D7$-branes and $O7$-planes in 1+1-dimensions. We use anomaly cancellation and string dualities to argue that there must be chiral fermion zero-modes on the $D3$-branes which are localized near the $O7$-planes. Away from the orientifold limit we verify this by using index theory as well as explicit construction of the zero-modes. This system is related to F-theory on K3 and heterotic matrix string theory, and the heterotic strings are related to Alice string defects in $\mathcal{N}=4$ Super-Yang-Mills. In the limit of large $N_c$ we find an $AdS_3$ dual of the heterotic matrix string CFT.Comment: 44 pages, typos corrected, version published in JHE

alpha'-exact entropies for BPS and non-BPS extremal dyonic black holes in heterotic string theory from ten-dimensional supersymmetry

We calculate near-horizon solutions for four-dimensional 4-charge and five-dimensional 3-charge black holes in heterotic string theory from the part of the ten-dimensional tree-level effective action which is connected to gravitational Chern-Simons term by supersymmetry. We obtain that the entropies of large black holes exactly match the alpha'-exact statistical entropies obtained from microstate counting (D=4) and AdS/CFT correspondence (D=5). Especially interesting is that we obtain agreement for both BPS and non-BPS black holes, contrary to the case of R^2-truncated (four-derivative) actions (D-dimensional N=2 off-shell supersymmetric or Gauss-Bonnet) were used, which give the entropies agreeing (at best) just for BPS black holes. The key property of the solutions, which enabled us to tackle the action containing infinite number of terms, is vanishing of the Riemann tensor \bar{R}_{MNPQ} obtained from torsional connection defined with \bar{\Gamma} = \Gamma - H/2. Morover, if every monomial of the remaining part of the effective action would contain at least two Riemanns \bar{R}_{MNPQ}, it would trivially follow that our solutions are exact solutions of the full heterotic effective action in D=10. The above conjecture, which appeared (in this or stronger form) from time to time in the literature, has controversial status, but is supported by the most recent calculations of Richards (arXiv:0807.3453 [hep-th]). Agreement of our results for the entropies with the microscopic ones supports the conjecture. As for small black holes, our solutions in D=5 still have singular horizons.Comment: 28 pages; v2: minor changes, references added; v3: extended discussion on small black holes in sec. 5.4, more references added, accepted in JHE

Near the horizon of 5D black rings

For the five dimensional N=2 black rings, we study the supersymmetry enhancement and identify the global supergroup of the near horizon geometry. We show that the global part of the supergroup is OSp(4*|2)X U(1) which is similar to the small black string. We show that results obtained by applying the entropy function formalism, the c-extremization approach and the Brown-Henneaux method to the black ring solution are in agreement with the microscopic entropy calculation.Comment: 26 pages, version to appear in JHEP, the near horizon superalgebra is corrected, discussion on small black ring is discarded, Brown-Henneaux approach to large black ring is adde

Fundamental Superstrings as Holograms

The worldsheet of a macroscopic fundamental superstring in the Green-Schwarz light-cone gauge is viewed as a possible boundary hologram of the near horizon region of a small black string. For toroidally compactified strings, the hologram has global symmetries of AdS_3 \times S^{d-1} \times T^{8-d}, (d =3,..,8), only some of which extend to local conformal symmetries. We construct the bulk string theory in detail for the particular case of d=3. The symmetries of the hologram are correctly reproduced from this exact worldsheet description in the bulk. Moreover, the central charge of the boundary Virasoro algebra obtained from the bulk agrees with the Wald entropy of the associated small black holes. This construction provides an exact CFT description of the near horizon region of small black holes both in Type-II and heterotic string theory arising from multiply wound fundamental superstrings.Comment: 46 pages, JHEP style. v2: Comments, references adde

D-Brane Propagation in Two-Dimensional Black Hole Geometries

We study propagation of D0-brane in two-dimensional Lorentzian black hole backgrounds by the method of boundary conformal field theory of SL(2,R)/U(1) supercoset at level k. Typically, such backgrounds arise as near-horizon geometries of k coincident non-extremal NS5-branes, where 1/k measures curvature of the backgrounds in string unit and hence size of string worldsheet effects. At classical level, string worldsheet effects are suppressed and D0-brane propagation in the Lorentzian black hole geometry is simply given by the Wick rotation of D1-brane contour in the Euclidean black hole geometry. Taking account of string worldsheet effects, boundary state of the Lorentzian D0-brane is formally constructible via Wick rotation from that of the Euclidean D1-brane. However, the construction is subject to ambiguities in boundary conditions. We propose exact boundary states describing the D0-brane, and clarify physical interpretations of various boundary states constructed from different boundary conditions. As it falls into the black hole, the D0-brane radiates off to the horizon and to the infinity. From the boundary states constructed, we compute physical observables of such radiative process. We find that part of the radiation to infinity is in effective thermal distribution at the Hawking temperature. We also find that part of the radiation to horizon is in the Hagedorn distribution, dominated by massive, highly non-relativistic closed string states, much like the tachyon matter. Remarkably, such distribution emerges only after string worldsheet effects are taken exactly into account. From these results, we observe that nature of the radiation distribution changes dramatically across the conifold geometry k=1 (k=3 for the bosonic case), exposing the `string - black hole transition' therein.Comment: 51 pages, 5 figures, v2: referece added, note added replying the comment made in hep-th/060206

String Theory on Warped AdS_3 and Virasoro Resonances

We investigate aspects of holographic duals to time-like warped AdS_3 space-times--which include G\"odel's universe--in string theory. Using worldsheet techniques similar to those that have been applied to AdS_3 backgrounds, we are able to identify space-time symmetry algebras that act on the dual boundary theory. In particular, we always find at least one Virasoro algebra with computable central charge. Interestingly, there exists a dense set of points in the moduli space of these models in which there is actually a second commuting Virasoro algebra, typically with different central charge than the first. We analyze the supersymmetry of the backgrounds, finding related enhancements, and comment on possible interpretations of these results. We also perform an asymptotic symmetry analysis at the level of supergravity, providing additional support for the worldsheet analysis.Comment: 24 pages + appendice

Computing the Spectrum of a Heterotic Flux Vacuum

We compute the massless spectra of a set of flux vacua of the heterotic string. The vacua we study include well-known non-Kahler T^2-fibrations over K3 with SU(3) structure and intrinsic torsion. Following gauged linear sigma models of these vacua into phases governed by asymmetric Landau-Ginzburg orbifolds allows us to compute the spectrum using generalizations of familiar LG-orbifold techniques. We study several four- and six-dimensional examples with N=2 spacetime supersymmetry in detail.Comment: 1+49 pages, updated to JHEP version (added appendix and minor revisions

Evaluation of methods for detection of fluorescence labeled subcellular objects in microscope images

<p>Abstract</p> <p>Background</p> <p>Several algorithms have been proposed for detecting fluorescently labeled subcellular objects in microscope images. Many of these algorithms have been designed for specific tasks and validated with limited image data. But despite the potential of using extensive comparisons between algorithms to provide useful information to guide method selection and thus more accurate results, relatively few studies have been performed.</p> <p>Results</p> <p>To better understand algorithm performance under different conditions, we have carried out a comparative study including eleven spot detection or segmentation algorithms from various application fields. We used microscope images from well plate experiments with a human osteosarcoma cell line and frames from image stacks of yeast cells in different focal planes. These experimentally derived images permit a comparison of method performance in realistic situations where the number of objects varies within image set. We also used simulated microscope images in order to compare the methods and validate them against a ground truth reference result. Our study finds major differences in the performance of different algorithms, in terms of both object counts and segmentation accuracies.</p> <p>Conclusions</p> <p>These results suggest that the selection of detection algorithms for image based screens should be done carefully and take into account different conditions, such as the possibility of acquiring empty images or images with very few spots. Our inclusion of methods that have not been used before in this context broadens the set of available detection methods and compares them against the current state-of-the-art methods for subcellular particle detection.</p

Image-Based Assessment of Growth and Signaling Changes in Cancer Cells Mediated by Direct Cell-Cell Contact

Many important biological processes are controlled through cell-cell interactions, including the colonization of metastatic tumor cells and the control of differentiation of stem cells within their niche. Despite the crucial importance of the cellular environment in regulating cellular signaling, in vitro methods for the study of such interactions are difficult and/or indirect.We report on the development of an image-based method for distinguishing two cell types grown in coculture. Furthermore, cells of one type that are in direct contact with cells of a second type (adjacent cells) can be analyzed separately from cells that are not within a single well. Changes are evaluated using population statistics, which are useful in detecting subtle changes across two populations. We have used this system to characterize changes in the LNCaP prostate carcinoma cell line when grown in contact with human vascular endothelial cells (HUVECs). We find that the expression and phosphorylation of WWOX is reduced in LNCaP cells when grown in direct contact with HUVECs. Reduced WWOX signaling has been associated with reduced activation or expression of JNK and p73. We find that p73 levels are also reduced in LNCaP cells grown in contact with HUVECs, but we did not observe such a change in JNK levels.We find that the method described is statistically robust and can be adapted to a wide variety of studies where cell function or signaling are affected by heterotypic cell-cell contact. Ironically, a potential challenge to the method is its high level of sensitivity is capable of classifying events as statistically significant (due to the high number cells evaluated individually), when the biological effect may be less clear. The methodology would be best used in conjunction with additional methods to evaluate the biological role of potentially subtle differences between populations. However, many important events, such as the establishment of a metastatic tumor, occur through rare but important changes, and methods such as we describe here can be used to identify and characterize the contribution of the environment to these changes