Ergonomy in laboratory animal care

No abstract availabl

Radio Galaxy Clustering at z~0.3

Radio galaxies are uniquely useful as probes of large-scale structure as their uniform identification with giant elliptical galaxies out to high redshift means that the evolution of their bias factor can be predicted. As the initial stage in a project to study large-scale structure with radio galaxies we have performed a small redshift survey, selecting 29 radio galaxies in the range 0.19<z<0.45 from a contiguous 40 square degree area of sky. We detect significant clustering within this sample. The amplitude of the two-point correlation function we measure is consistent with no evolution from the local (z<0.1) value. This is as expected in a model in which radio galaxy hosts form at high redshift and thereafter obey a continuity equation, although the signal:noise of the detection is too low to rule out other models. Larger surveys out to z~1 should reveal the structures of superclusters at intermediate redshifts and strongly constrain models for the evolution of large-scale structure.Comment: 7 pages, 3 figures, accepted by ApJ Letter

Colorectal Cancer Screening Practices among Obstetrician/Gynecologists and Nurse Practitioners

Abstract Objective: Obstetrician/gynecologists (Ob/Gyn) and nurse practitioners (NP) are essential providers of primary and preventive care for their female patients. Therefore, colorectal cancer (CRC) screening should be part of their routine preventive practices. The purpose of our study is to evaluate the CRC screening practices of these providers. Methods: A self-administered survey was mailed to a national sample of 1130 Ob/Gyns and NPs to assess providers' demographics, current CRC screening practices, and familiarity with CRC guidelines. Results: Three hundred thirty-six providers (29.7%) returned our survey (54% Ob/Gyns and 46% NPs). Three fourths of providers routinely performed screening for CRC, compared with 95% for breast and cervical cancer. Routine CRC screening was more common among Ob/Gyns (87.2%) than NPs (61.7%) (p10 years (p<0.01), practicing in a multispecialty group (2.62 times more likely), and having an older patient population (p<0.001). Conclusions: Ob/Gyns and NPs underuse CRC screening compared with breast and cervical cancer screening and lack knowledge about appropriate use of CRC screening modalities. Opportunities to further educate Ob/Gyns and NPs should be sought to improve compliance with current CRC screening guidelines.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/78151/1/jwh.2008.1117.pd

COVID-19 and Preventing Harm to Vulnerable Children

Although COVID-19 mercifully seems to affect children less severely than adults, children are far from immune from the impacts of the virus. Public health orders closing schools and businesses, cancelling events, and keeping children at home have been disruptive and distressing to many children and families. But for children who rely on government entities for protection, care, custody, and services, the effects of the public health orders can be devastating. COVID-19 and the response to it has serious implications for the safety, well-being, and development of these vulnerable children—those within the child welfare, juvenile justice, and special education systems. All three groups consist of children to whom the state has legal obligations. Additionally, all three groups consist disproportionately of children of color, a reality being brought to the forefront in the context of this pandemic and beyond. As these child-serving systems adapt to the new realities defined by public health limitations, there exists an opportunity to address both immediate challenges as well as enduring concerns within these complex structures. This Article explores the current state of child rights within the child welfare, juvenile justice, and special education systems, highlighting concerns that pre-date COVID-19 as well as recent legal implications of the pandemic. Each section examines the particular repercussions of the pandemic and the response to it on children and proposes potential remedies. It also offers perspective on how meeting today’s critical challenges can result in long-term systemic improvements

Differential neuroproteomic and systems biology analysis of spinal cord injury

Acute spinal cord injury (SCI) is a devastating condition with many consequences and no known effective treatment. Although it is quite easy to diagnose traumatic SCI, the assessment of injury severity and projection of disease progression or recovery are often challenging, as no consensus biomarkers have been clearly identified. Here rats were subjected to experimental moderate or severe thoracic SCI. At 24h and 7d postinjury, spinal cord segment caudal to injury center versus sham samples was harvested and subjected to differential proteomic analysis. Cationic/anionic-exchange chromatography, followed by 1D polyacrylamide gel electrophoresis, was used to reduce protein complexity. A reverse phase liquid chromatography-tandem mass spectrometry proteomic platform was then utilized to identify proteome changes associated with SCI. Twenty-two and 22 proteins were up-regulated at 24 h and 7 day after SCI, respectively; whereas 19 and 16 proteins are down-regulated at 24 h and 7 day after SCI, respectively, when compared with sham control. A subset of 12 proteins were identified as candidate SCI biomarkers - TF (Transferrin), FASN (Fatty acid synthase), NME1 (Nucleoside diphosphate kinase 1), STMN1 (Stathmin 1), EEF2 (Eukaryotic translation elongation factor 2), CTSD (Cathepsin D), ANXA1 (Annexin A1), ANXA2 (Annexin A2), PGM1 (Phosphoglucomutase 1), PEA15 (Phosphoprotein enriched in astrocytes 15), GOT2 (Glutamic-oxaloacetic transaminase 2), and TPI-1 (Triosephosphate isomerase 1), data are available via ProteomeXchange with identifier PXD003473. In addition, Transferrin, Cathepsin D, and TPI-1 and PEA15 were further verified in rat spinal cord tissue and/or CSF samples after SCI and in human CSF samples from moderate/severe SCI patients. Lastly, a systems biology approach was utilized to determine the critical biochemical pathways and interactome in the pathogenesis of SCI. Thus, SCI candidate biomarkers identified can be used to correlate with disease progression or to identify potential SCI therapeutic targets

The Processing Pathway of Prelamin A

The conversion of mammalian prelamin A to mature lamin A proceeds through the removal of 18 amino acids from the carboxyl terminus. The initial step in this processing is the isoprenylation of a CAAX box cysteine. This proteolytic event is distinctive for prelamin A among the known prenylated mammalian proteins. Since the carboxyl terminus of prelamin A is removed during maturation, it is not obvious that this protein would undergo the two reactions subsequent to prenylation observed in other CAAX box proteins-the endoproteolytic removal of the carboxyl-terminal 3 amino acids and the subsequent methylation of the now carboxyl-terminal cysteine. To characterize the maturation of prelamin A further, we have developed a CHO-K1 cell line that possesses a dexamethasone-inducible human prelamin A against a genetic background of high mevalonate uptake. Utilizing this cell line in association with antibodies specific to the transgenic prelamin A, we have been able to demonstrate directly in vivo that prelamin A undergoes farnesylation and carboxymethylation prior to conversion to lamin A, as is the case for other prenylated proteins. We have demonstrated previously that in the absence of isoprenylation, conversion of prelamin A to lamin A is blocked, but that unprocessed prelamin A is transported to the nucleus where it can still undergo maturation. Consistent with the implications of these prior studies, we now demonstrate the presence of both subunits of farnesyl-protein transferase in the nucleus

Understanding solvent spreading for Langmuir deposition of nanomaterial films: a Hansen solubility parameter approach

In order to prepare high-quality Langmuir films of two-dimensional materials it is important to select a solvent optimized for both exfoliation and spreading at the air-water interface. Whilst it is generally accepted that exfoliation and stabilisation of two-dimensional materials is well-described using the Hansen solubility parameter theory, a complementary description of solvent spreading behaviour is lacking. To this end we develop an understanding of solvent spreading using a Hansen solubility parameter framework. Our model accurately predicts the behaviour of both water-immiscible and water-miscible solvents in Langmuir film formation experiments. We demonstrate that spreading behaviour can be modified by controlling the surface pressure of the subphase using an amphiphilic species and accordingly utilise this approach to determine the maximum spreading pressure for a selection of solvents. Ultimately, by building on this understanding we open up additional routes to optimize the preparation of Langmuir films of two-dimensional materials and other nanoparticles

Theory of Pseudomodes in Quantum Optical Processes

This paper deals with non-Markovian behaviour in atomic systems coupled to a structured reservoir of quantum EM field modes, with particular relevance to atoms interacting with the field in high Q cavities or photonic band gap materials. In cases such as the former, we show that the pseudo mode theory for single quantum reservoir excitations can be obtained by applying the Fano diagonalisation method to a system in which the atomic transitions are coupled to a discrete set of (cavity) quasimodes, which in turn are coupled to a continuum set of (external) quasimodes with slowly varying coupling constants and continuum mode density. Each pseudomode can be identified with a discrete quasimode, which gives structure to the actual reservoir of true modes via the expressions for the equivalent atom-true mode coupling constants. The quasimode theory enables cases of multiple excitation of the reservoir to now be treated via Markovian master equations for the atom-discrete quasimode system. Applications of the theory to one, two and many discrete quasimodes are made. For a simple photonic band gap model, where the reservoir structure is associated with the true mode density rather than the coupling constants, the single quantum excitation case appears to be equivalent to a case with two discrete quasimodes

Non-Markovian Decay of a Three Level Cascade Atom in a Structured Reservoir

We present a formalism that enables the study of the non-Markovian dynamics of a three-level ladder system in a single structured reservoir. The three-level system is strongly coupled to a bath of reservoir modes and two quantum excitations of the reservoir are expected. We show that the dynamics only depends on reservoir structure functions, which are products of the mode density with the coupling constant squared. This result may enable pseudomode theory to treat multiple excitations of a structured reservoir. The treatment uses Laplace transforms and an elimination of variables to obtain a formal solution. This can be evaluated numerically (with the help of a numerical inverse Laplace transform) and an example is given. We also compare this result with the case where the two transitions are coupled to two separate structured reservoirs (where the example case is also analytically solvable)