Oxytocin at physiological concentrations evokes adrenocorticotropin (ACTH) release from corticotrophs by increasing intracellular free calcium mobilized mainly from intracellular stores. Oxytocin displays synergistic or additive effects on ACTH-releasing factor or arginine vasopressin-induced ACTH secretion, respectively

The potency of oxytocin (OT) in evoking ACTH secretion by isolated, superfused rat adenohypophyseal corticotrophs and its enhancement by CRF and arginine vasopressin (AVP) were analyzed. Each secretagogue effectively released ACTH from adenohypophyseal cells when added separately in pulsatile fashion in physiological concentrations based on hypophyseal portal blood (OT, 10 nM; AVP, 0.5 nM; CRF, 0.1 nM). OT released ACTH at concentrations as low as 1 nM. Moreover, a dose- response relationship up to 10 microM was revealed. Combinations of a constant amount of CRF (0.1 nM) with increasing concentrations of OT exerted a synergistic effect on ACTH release. In contrast, OT given in various concentrations in combination with AVP (0.5 nM) produced an additive effect on ACTH release. To study the mechanism of action of OT on ACTH secretion, cytosolic free calcium levels in single pituitary cells exposed to OT or AVP were measured using the calcium-sensitive fluorescent indicator Fura-2. Corticotrophs among mixed adenohypophyseal cell types in the primary cultures were identified by immunocytochemistry. More than 500 cells were individually stimulated with OT or AVP. Basal cytosolic free calcium levels ranged between 80- 130 nM free calcium. The addition of 100 nM OT or 1 microM AVP increased the cytosolic free calcium concentration within 3 sec to values ranging from 500-800 nM. An increase in intracellular calcium ranging from 200-500 nM due to OT could still be observed after extracellular calcium depletion. Taken together, our data demonstrate that physiological concentrations of OT stimulate ACTH secretion, independent of the other ACTH secretagogues, by mobilizing calcium mainly from intracellular stores

PSR B1828-11: a precession pulsar torqued by a quark planet?

The pulsar PSR B1828-11 has long-term, highly periodic and correlated variations in both pulse shape and the rate of slow-down. This phenomenon may provide evidence for precession of the pulsar as suggested previously within the framework of free precession as well as forced one. On a presumption of forced precession, we propose a quark planet model to this precession henomenon instead, in which the pulsar is torqued by a quark planet. We construct this model by constraining mass of the pulsar ($M_{\rm psr}$), mass of the planet ($M_{\rm pl}$) and orbital radius of the planet ($r_{\rm pl}$). Five aspects are considered: derived relation between $M_{\rm psr}$ and $r_{\rm pl}$, movement of the pulsar around the center of mass, ratio of $M_{\rm psr}$ and $M_{\rm pl}$, gravitational wave radiation timescale of the planetary system, and death-line criterion. We also calculate the range of precession period derivative and gravitational wave strength (at earth) permitted by the model. Under reasonable parameters, the observed phenomenon can be understood by a pulsar ($10^{-4}\sim10^{-1}M_{\odot}$) with a quark planet ($10^{-8}\sim10^{-3}M_{\odot}$) orbiting it. According to the calculations presented, the pulsar would be a quark star because of its low mass, which might eject a lump of quark matter (to become a planet around) during its birth.Comment: 6 pages, 3 figures, accepted by MNRAS (Letters

A new approach to anti-neutrino running in long baseline neutrino oscillation experiments

We study the possibility to replace the anti-neutrino run of a long baseline neutrino oscillation experiment, with anti-neutrinos from muon decay at rest. The low energy of these neutrinos allows the use of inverse beta decay for detection in a Gadolinium-doped water Cerenkov detector. We show that this approach yields a factor of five times larger anti-neutrino event sample. The resulting discovery reaches in theta_13, the mass hierarchy and leptonic CP violation are compared with those from a conventional superbeam experiment with combined neutrino and anti-neutrino running. We find that this approach yields a greatly improved reach for CP violation and theta_13 while leaving the ability to measure the mass hierarchy intact.Comment: 4 pages, 2 pages, 1 tabl

The Rwenzori Mountains, a Paleoproterzoic crustal shear belt crossing the Albertine rift system

This contribution discusses the development of the Paleoproterozoic Buganda-Toro belt in the Rwenzori mountains and its influence on the western part of the East African Rift System in Uganda. The Buganda-Toro belt is composed of several thick-skinned nappes consisting of Archaean Gneisses and Palaeoproterozoic cover units that are thrusted northwards. The high Rwenzori mountains are located in the frontal unit of this belt with retrograde greenschist facies gneisses towards the north, which are unconformably overlain by metasediments and amphibolites. Towards the south the metasediments are overthrust by the next migmatitic gneiss unit that belongs to a crustal scale nappe. The southwards dipping metasedimentary and volcanic sequence in the high Rwenzori mountains shows an inverse metamorphic grade with greenschist facies conditions in the north and amphibolite facies conditions in the south. Early D1 deformation structures are overgrown by cordierite, which in turn grows into D2 deformation, representing the major northwards directed thrusting event. We argue that the inverse metamorphic gradient develops because higher grade rocks are exhumed in the footwall of a crustal scale nappe whereas the exhumation decreases towards the north away from the nappe leading to a decrease in metamorphic grade. The D2 deformation event is followed by a D3 E-W compression, a D4 with the development of steep shear zones with a NNE-SSW and SSE-NNW trend including the large Nyamwamba shear followed by a local D5 retrograde event and D6 brittle inverse faulting. The Paleoproterozoic Buganda-Toro belt is relatively stiff and crosses the NNE-SSW running rift system exactly at the node where the highest peaks of the Rwenzori mountains are situated and where the lake George rift terminates towards the north. Orientation of brittle and ductile fabrics show some similarities indicating that the cross-cutting Buganda-Toro belt influenced rift propagation and brittle fault development within the Rwenzori mountain and that this stiff belt may form part of the reason why the Rwenzori mountains are relatively high within the rift. Keywords: East African Rift, Basement, Buganda Toro, Inverse Metamorphic Gradient, Microtectonics, Rwenzori mountain

Remote sensing of aquatic plants

Various sensors were tested in terms of their ability to detect and discriminate among noxious aquatic macrophytes. A survey of researchers currently studying the problem and a brief summary of their work is included. Results indicated that the sensor types best suited to assessment of the aquatic environment are color, color infrared, and black-and-white infrared film, which furnish consistently high contrasts between aquatic plants and their surroundings

Effect of oxytocin on free intracellular Ca2+ levels and progesterone release by human granulosa-lutein cells

Oxytocin and its receptor are found in the corpus luteum in a variety of species, including the human. In the present study we used fura-2 microfluorimetry to investigate whether activation of the oxytocin receptor of cultured human granulosa-lutein cells causes intracellular calcium (Ca2+) signals and affects progesterone release. Although after 1 day in culture, cells were not responsive to oxytocin, the number of responsive cells increased steadily during the first 3 days in culture, reaching a maximum on days 4 and 5 (59-66%) and then declined again until day 8. Effective oxytocin concentrations were apparently independent of the culture day, and concentrations as low as 10 nmol/L increased intracellular free Ca2+ levels from 70-140 nmol/L (basal levels) to maximal peak levels of 800 nmol/L. The oxytocin-induced Ca2+ signal was not affected by removal of extracellular Ca2+ with EGTA. Moreover, depletion of intracellular Ca2+ stores by ionomycin treatment rendered the cells unresponsive to oxytocin, pointing also at the intracellular source of the oxytocin-inducible Ca2+ signal. Interestingly, after one single stimulation with oxytocin, cells became refractory to additional stimuli, and only extremely high concentrations of oxytocin induced a second increase in intracellular free Ca2+. To examine the possible effects of oxytocin on progesterone release by cultured cells, we incubated cells on culture day 2 (20% responsive cells in the fura measurements) and culture day 5 (66% responsive cells in the fura measurements) for 24 h with oxytocin (10 nmol/L) and hCG (10,000 IU/L). Although hCG significantly stimulated progesterone release, oxytocin alone was without a stimulatory effect on either day. However, a significant augmentation of the effect of hCG on progesterone release was found in incubations of cells on day 5. Interestingly, the effects of hCG also included stimulation of oxytocin release by cultured granulosa-lutein cells into the culture medium, as determined by RIA. In summary, our data indicate the presence of a functional oxytocin receptor on human granulosa-lutein cells that is linked to Ca2+ as a second messenger released from intracellular Ca2+ stores. The number of oxytocin-responsive cells increases during differentiation in culture. Moreover, oxytocin release induced by hCG and a stimulatory effect of oxytocin on the hCG-induced progesterone production during the period of maximal responsiveness of cultured cells were found. We, therefore, propose that oxytocin may have autocrine and/or paracrine functions in human granulosa-lutein cells, including fine-tuning of progesterone release

Magnetic excitations in the metallic single-layer Ruthenates Ca(2-x)Sr(x)RuO(4) studied by inelastic neutron scattering

By inelastic neutron scattering, we have analyzed the magnetic correlations in the paramagnetic metallic region of the series Ca(2-x)Sr(x)RuO(4), 0.2<=x<=0.62. We find different contributions that correspond to 2D ferromagnetic fluctuations and to fluctuations at incommensurate wave vectors (0.11,0,0), (0.26,0,0) and (0.3,0.3,0). These components constitute the measured response as function of the Sr-concentration x, of the magnetic field and of the temperature. A generic model is applicable to metallic Ca(2-x)Sr(x)RuO(4) close to the Mott transition, in spite of their strongly varying physical properties. The amplitude, characteristic energy and width of the incommensurate components vary only little as function of x, but the ferromagnetic component depends sensitively on concentration, temperature and magnetic field. While ferromagnetic fluctuations are very strong in Ca1.38Sr0.62RuO4 with a low characteristic energy of 0.2 meV at T=1.5 K, they are strongly suppressed in Ca1.8Sr0.2RuO4, but reappear upon the application of a magnetic field and form a magnon mode above the metamagnetic transition. The inelastic neutron scattering results document how the competition between ferromagnetic and incommensurate antiferromagnetic instabilities governs the physics of this system

Interpreting experimental bounds on D^0 - \bar{D^0} mixing in the presence of CP violation

We analyse the most recent experimental data regarding D^0 - \bar{D^0} mixing, allowing for CP violation. We focus on the dispersive part of the mixing amplitude, M^D_{12}, which is sensitive to new physics contributions. We obtain a constraint on the mixing amplitude: |M^D_{12}| < 6.2\times 10^{-11} MeV at 95% C.L. . This constraint is weaker by a factor of about three than the one which is obtained when no CP violation is assumed.Comment: 9 pages, revtex4; One reference updated, one reference added, footnote 3 correcte

TeV neutrinos and gamma rays from pulsars

Recent studies suggest that pulsars could be strong sources of TeV muon neutrinos provided positive ions are accelerated by pulsar polar caps to PeV energies. In such a situation muon neutrinos are produced through the delta resonance in interactions of pulsar accelerated ions with its thermal radiation field. High energy gamma rays also should be produced simultaneously in pulsar environment as both charged and neutral pions are generated in the interactions of energetic hadrons with the ambient photon fields. Here we estimate TeV gamma ray flux at Earth from few nearby young pulsars. When compared with the observations we find that proper consideration of the effect of polar cap geometry in flux calculation is important. Incorporating such an effect we obtain the (revised) event rates at Earth due to few potential nearby pulsars. The results suggest that pulsars are unlikely to be detected by the upcoming neutrino telescopes. We also estimate TeV gamma ray and neutrino fluxes from pulsar nebulae for the adopted model of particle acceleration.Comment: Six pages, accepted in MNRA