Biological Microscopy with Undetected Photons

Novel imaging techniques utilizing nondegenerate, correlated photon pairs sparked intense interest during the last couple of years among scientists of the quantum optics community and beyond. It is a key property of such "ghost imaging" or "quantum interference" methods that they use those photons of the correlated pairs for imaging that never interacted with the sample, allowing detection in a spectral range different from that of the illumination of the object. Extensive applications of these techniques in spectroscopy and microscopy are envisioned, however, their limited spatial resolution to date has not yet supported real-life microscopic investigations of tiny biological objects. Here we report a modification of the method based on quantum interference by using a seeding laser and confocal scanning, that allows the improvement of the resolution of imaging with undetected photons by more than an order of magnitude, and we also present examples of application in the microscopy of biological samples.Comment: This article has been accepted for publication in IEEE Access, but has not been fully edite

The effect of four-phasic versus three-phasic contrast media injection protocols on extravasation rate in coronary CT angiography: a randomized controlled trial.

OBJECTIVES: Contrast media (CM) extravasation is a well-known complication of CT angiography (CTA). Our prospective randomized control study aimed to assess whether a four-phasic CM administration protocol reduces the risk of extravasation compared to the routinely used three-phasic protocol in coronary CTA. METHODS: Patients referred to coronary CTA due to suspected coronary artery disease were included in the study. All patients received 400 mg/ml iomeprol CM injected with dual-syringe automated injector. Patients were randomized into a three-phasic injection-protocol group, with a CM bolus of 85 ml followed by 40 ml of 75%:25% saline/CM mixture and 30 ml saline chaser bolus; and a four-phasic injection-protocol group, with a saline pacer bolus of 10 ml injected at a lower flow rate before the three-phasic protocol. RESULTS: 2,445 consecutive patients were enrolled (mean age 60.6 +/- 12.1 years; females 43.6%). Overall rate of extravasation was 0.9% (23/2,445): 1.4% (17/1,229) in the three-phasic group and 0.5% (6/1,216) in the four-phasic group (p = 0.034). CONCLUSIONS: Four-phasic CM administration protocol is easy to implement in the clinical routine at no extra cost. The extravasation rate is reduced by 65% with the application of the four-phasic protocol compared to the three-phasic protocol in coronary CTA. KEY POINTS: * Four-phasic CM injection-protocol reduces extravasation rate by 65% compared to three-phasic. * The saline pacer bolus substantially reduces the risk of CM extravasation. * The implementation of four-phasic injection-protocol is at no cost

Terrestrial and aquatic ecosystem responses to early Holocene rapid climate change (RCC) events in the South Carpathian Mountains, Romania

As demonstrated by an increasing number of palaeoclimatic and palaeoecological studies, rapid climate change events (RCCs) occurred frequently in the Holocene and their timing correlates well in the European records. Changes in vegetation composition and environmental conditions were significant during these RCC events. In this study we use high resolution pollen, stomata, micro- and macrocharcoal, macrofossil, siliceous algae, biogenic silica and organic content analyses from two alpine lake sediments (Lake Brazi, 1740 m a.s.l.; Lake Gales, 1990 m a.s.l.) in the Retezat Mts, South Carpathian Mountains, Romania. Our aim is to study ecosystem responses to RCCs between 12,000 and 7000 cal yr BP using high-resolution proxy analyses of the relevant sediment sections. We detected several significant changes in the terrestrial vegetation composition and aquatic ecosystems in case of both lakes. Complex ecosystem responses were found in connection with the early Holocene RCC intervals. Most prominently, the 10.2 ka climatic change likely fostered the extinction of Larix decidua from the shore of Lake Brazi, while climatic change during the 8.2 ka event facilitated the establishment of Carpinus betulus in the lower deciduous mixed oak forests, due to decreasing growing season temperatures, frequent summer droughts and associated recurrent fire events. Taken together, most of the significant pollen compositional changes reflected the periodic spread of pioneer deciduous tree taxa (mainly Fraxinus excelsior and Corylus avellana) during the early Holocene RCCs. In all cases, this change was connectable to increased regional fire activity and the temporary increase of herbs. The most significant change in the lake-ecosystems was often the short-lived spread of various planktonic diatom species, mainly the members of genus Aulacoseira. Sudden appearance and large-scale percentage increase of these taxa suggested higher water-depth and/or intensified water turbulence

Warm Younger Dryas summers and early late glacial spread of temperate deciduous trees in the Pannonian Basin during the last glacial termination (20-9 kyr cal BP)

This paper focuses on the last glacial termination vegetation and climate reorganization of the Eastern Pannonian Plain via the multi-proxy paleoecological analysis of Kokad mire in Eastern Hungary. Grains size, sediment chemistry and magnetic susceptibility records are compared with the pollen and plant macrofossil records on the basis of which biome and quantitative summer mean temperature reconstructions are provided and discussed with other climate records of the region. Biome assignments indicated (graminoid and forb) tundra vegetation from 19,440 cal yr BP with the local presence of Betula pendula, B. pubescens, B. nana, and Pinus sylvestris. A rapid biome shift to cool coniferous forest took place at 16,200 cal yr BP, and a second biome shift was identified at 14,740 cal yr BP when cool mixed forest developed and persisted into the Early Holocene. The most prominent feature of the record was the early post last glacial maximum (LGM) establishment (17,700 cal yr BP) and expansion (14,700 cal yr BP) of elm (Ulmus) and hazel (Corylus) supporting the phylogeographical evidence for extra-Mediterranean refugia in the Pannonian Basin. The fungal spore record indicated the presence of large grazing mammals locally after the LGM until ∼16,780 cal yr BP. Their disappearance predated the biome shift to cold coniferous forest and the final increase of forest fires. Pollen based summer temperature reconstruction suggested relatively warm summers (∼14.5 °C) by 19,440 cal yr BP, and buffered July mean temperature fluctuations throughout the last glacial termination in this region with < 1 °C decrease in summer mean temperatures during the Younger Dryas stadial, and ∼2.2 °C warming in the Early Holocene. Our comprehensive summary of the basin’s Late Pleniglacial (24,000–14,600 cal yr BP) and late glacial paleoenvironmental and paleoclimate records showed a partially wooded landscape with higher woody cover in Western Hungary. Several loess and lake archives confirmed that in this region of Europe the warming after Heinrich event 1 (around 16,200 cal yr BP) had similar amplitude to the late glacial warming

Telmisartan Is a Promising Agent for Managing Neuropathic Pain and Delaying Opioid Analgesic Tolerance in Rats

Despite the large arsenal of analgesic medications, neuropathic pain (NP) management is not solved yet. Angiotensin II receptor type 1 (AT1) has been identified as a potential target in NP therapy. Here, we investigate the antiallodynic effect of AT1 blockers telmisartan and losartan, and particularly their combination with morphine on rat mononeuropathic pain following acute or chronic oral administration. The impact of telmisartan on morphine analgesic tolerance was also assessed using the rat tail-flick assay. Morphine potency and efficacy in spinal cord samples of treated neuropathic animals were assessed by [35S]GTPγS-binding assay. Finally, the glutamate content of the cerebrospinal fluid (CSF) was measured by capillary electrophoresis. Oral telmisartan or losartan in higher doses showed an acute antiallodynic effect. In the chronic treatment study, the combination of subanalgesic doses of telmisartan and morphine ameliorated allodynia and resulted in a leftward shift in the dose–response curve of morphine in the [35S]GTPγS binding assay and increased CSF glutamate content. Telmisartan delayed morphine analgesic-tolerance development. Our study has identified a promising combination therapy composed of telmisartan and morphine for NP and opioid tolerance. Since telmisartan is an inhibitor of AT1 and activator of PPAR-γ, future studies are needed to analyze the effect of each component