Molecular Gas during the Post-Starburst Phase: Low Gas Fractions in Green Valley Seyfert Post-Starburst Galaxies

Post-starbursts (PSBs) are candidate for rapidly transitioning from star-bursting to quiescent galaxies. We study the molecular gas evolution of PSBs at z ~ 0.03 - 0.2. We undertook new CO (2-1) observations of 22 Seyfert PSBs candidates using the ARO Submillimeter Telescope. This sample complements previous samples of PSBs by including green valley PSBs with Seyfert-like emission, allowing us to analyze for the first time the molecular gas properties of 116 PSBs with a variety of AGN properties. The distribution of molecular gas to stellar mass fractions in PSBs is significantly different than normal star-forming galaxies in the COLD GASS survey. The combined samples of PSBs with Seyfert-like emission line ratios have a gas fraction distribution which is even more significantly different and is broader (~ 0.03-0.3). Most of them have lower gas fractions than normal star-forming galaxies. We find a highly significant correlation between the WISE 12 micron to 4.6 micron flux ratios and molecular gas fractions in both PSBs and normal galaxies. We detect molecular gas in 27% of our Seyfert PSBs. Taking into account the upper limits, the mean and the dispersion of the distribution of the gas fraction in our Seyfert PSB sample are much smaller (mean = 0.025, std dev. = 0.018) than previous samples of Seyfert PSBs or PSBs in general (mean ~ 0.1 - 0.2, std dev. ~ 0.1 - 0.2).Comment: 17 pages, 12 figures accepted in MNRA

Effect of Leucaena leucocephala, as a protein source in a total mixed ration, on milk yield and composition of Saanen milk goats

This study examined the effects of diets with two protein sources (oilseed cake meal (OSCM) and Leucaena leucocephala hay) on the performance of lactating goats that were fed a total mixed ration (TMR). Thirty Saanen milk goats were divided into two groups, which were fed one of the two TMRS, with or without Leucaena hay at 25% of the DM. The TMRs were formulated to be iso-nitrogenous, iso-energetic and iso-neutral detergent fibre, while using two sources of crude protein, namely i) Leucaena hay; and ii) oilseed cake meal OSCM from soybean, sunflower, and cottonseed. No significant differences were revealed in body condition score, bodyweight change, milk yield and milk composition, such as milk fat (%), milk lactose (%), somatic cell count (x 103 cells/mL) and milk protein (%) during the entire lactation period. In contrast, goats fed the TMR with Leucaena hay had a lower milk urea nitrogen (mg nitrogen/dL) level than those fed the control diet (with OSCM). It was concluded that replacing a proportion of oilseed cake meal with Leucaena in Saanen milk goats fed TMR does not negatively influence milk production and composition.Keywords: fodder tree, oilseed cake, protein concentrate, somatic cell coun

THERAPEUTIC EFFECT OF SILVER NANOPARTICLES AGAINST DIETHYL NITROSAMIN AND CARBON TETTRACHLORIDE-INDUCED HEPATOCELLULAR CARCINOMA IN RATS

Objective: Hepatic cancer is known as primary liver cancer and hepatocellular carcinoma (HCC). Newly silver nanoparticles gained importance due to its advantages and multiple potential such as molecular imaging agent, antimicrobial, wound healing, anti-inflammatory and anticancer activity. The current study deals to assess therapeutic property silver nanoparticles (AgNPs) against diethylnitrosamine (DENA), and carbon tetrachloride (CCL4) induced hepatic cancer. Methods: Thirty male albino rats (200-250g) were distributed into four groups and hepatic cancer was induced with a single intraperitoneal dose of 200 mg/kg body weight of DENA. Two weeks later, animals received subcutaneous injections of CCl4 once a week in a dose of 3 ml/kg body weight for 6weeks. Serum biomarkers, antioxidants enzymes, inflammatory markers were evaluated to find the anti-proliferative potential of silver nanoparticles. Histological evaluation and microscopic reports were also done to document the results of the current work. Results: AgNPs significantly recover the serum marker enzymes of hepatic parameter AST, ALT, ALP, and total bilirubin and also decreased the levels of NO, IL-6 and TNF-α. Histopathological features also exhibited recovery of a hepatic architecture in cancer-induced rats. Moreover, the immunohistochemical investigation demonstrated that the levels of PCNA, and Caspase-3, which are hepatocarcinogenic markers, were significantly improved by AgNPs. Conclusion: These results concluded that AgNPs showed promising curing effects on hepatocellular ailments

Digestibility and rumen fermentation of a high forage diet pre-treated with a mixture of cellulase and xylanase enzymes

El articulo esta publicado en una revista de Open accessForages play an important role in ruminant animal production worldwide. Unlocking the nutritional potential of poor-quality tropical forages with fibrolytic enzymes would improve forage digestibility and utilization. Using in vitro and in vivo methods this study investigated the effect of pre-treating Smutsfinger hay for 24 hours with a mixture of fibrolytic enzyme (100% cellulase; 75% cellulase: 25% xylanase; 50% cellulase: 50% xylanase; 25% cellulase: 75% xylanase; 100% xylanase and a control with no enzyme) on ruminal fermentation and digestibility of nutrients by sheep. For in vitro fermentation, dry matter, neutral detergent fibre (NDF) degradability and volatile fatty acids (VFA) were determined with standard procedures. The same treatments were used for an in vivo digestibility trial using Merino sheep in a 6 x 6 Latin square design. Feed intake and total tract digestibility were recorded. Rumen fluid samples were collected daily, preserved, and analysed for VFA. The addition of 100% cellulase enzyme to Smutsfinger hay in vitro increased (P <0.05) NDF degradability and gas production compared with the control and inclusion of 100% xylanase enzyme. Both 100% cellulase and xylanase enzymes significantly reduced in vitro end time fermentation pH. A 50:50 mixture of cellulase and xylanase plus enzyme in vivo, increased acetate, total VFA concentration, and higher NDF and ADF digestibility of the test feed compared with the control. Inclusion of a 50-75% mixture of cellulase and 50-25% xylanase enzymes treatment led to higher gas production and butyrate concentration, decreased ruminal pH and improved nutrient digestibility

Perceptions of Gauteng Beef Farmers on Significance of Practising Climate Smart Agriculture

Farmers are crucial role-players in agriculture, especially in beef farming. Daily farm activities affect climate change, either negatively or positively. Therefore, farmers’ ability to relate climate change with farm activities is highly imperative. A study was conducted to investigate perceptions of Gauteng beef farmers on significance of practising climate smart agriculture (CSA). Semi-structured key informant interviews were conducted with 57 beef cattle farmers from three areas (Bronkhorstspruit, Rust de Winter and Cullinan) of Tshwane region (Gauteng province). A fully detailed ethical statement was used to explain the study and request farmers’ participation. Data analysis was done using a Statistical Package for Social Sciences (SPSS) software version 20 with a significance of P \u3c 0.05. Majority (71%) of farmers who participated were males. Most (43%) of participated farmers were middle age, which indicates that farmers are now considering beef farming as a full-time job. Majority (60%) of participants had access to enough land (\u3e 700 hectares), which makes them suitable for practising CSA, provided they get appropriate training. Few participants showed good understanding of climate change (14%), global warming (14%), climate change reduction strategies (29%), cattle contribution to climate change (14%), adaptation strategies for climate change (29%) and the role played by CSA on reduction of farm operational costs (14%). Majority of farmers had average understanding of climate change (86%), global warming (86%), cattle contribution on global warming (71%), climate change adaptation strategies (71%), climate change reduction strategies (71%), recommendation of climate smart feed resource (71%) and impact of CSA on economic development (86%). Majority (71%) of participants identified water pollution as the only environmental hazard associated with beef farming, whereas few (29%) identified air pollution due to greenhouse gases emissions from poorly managed cattle manure. All participants (100%) showed good understanding regarding the benefits of practising CSA and its impact on food security. Furthermore, they were willing to adopt CSA and promote it to fellow farmers. There is a need for farmers’ training on CSA

Detection of Inositol Polyphosphates by Polyacrylamide Gel Electrophoresis (PAGE) under Apoptotic Conditions in Cultured SW480 Cells

Inositol phosphates are naturally occurring compounds that regulate diverse cellular processes including apoptosis. Apoptosis is a mechanism by which cells undergo natural death to maintain cellular homeostasis. It causes cell death in areas during a state that is harmful to the body. It also regulates cellular development. Previous work has shown that exogenously administered, as well as endogenously manipulated inositol phosphates bring about apoptotic changes. It has been demonstrated that cellular levels of inositol phosphates, particularly higher inositol phosphates such as inositol hexakis-phosphate (IP6) and diphosphoinositol pentakis-phosphate (IP7) levels increase during apoptotic conditions. In this study, we have attempted to separate and identify higher inositol phosphates such as IP6 by polyacrylamide gel electrophoresis (PAGE) and shown that changes in inositol phosphate levels can be detected by this method. Cells were treated with etoposide to induce apoptosis, and apoptotic cells were observed under UV light following ethidium bromide/acridine orange staining. This staining showed that IP3 - IP6 induced apoptosis in SW480 cells with IP6 being the most effective inducing agent. The extracts from apoptotic and control cells were then loaded onto the polyacrylamide gel and run along with standard IP6. Results showed that IP6 could be detected using the PAGE method and that cellular levels of IP6 were increased in SW480 cells, in which apoptosis had been induced by etoposide. Our results demonstrated that this technique could be utilized instead of the laborious radioactive labeling and HPLC separation method to study the changes in cellular levels of inositol phosphates particularly IP6

Husbandry practices and phenotypic characteristics of indigenous goat populations in Ethiopia.

Abstract: The present study was conducted with the objective to characterize the husbandry practices and phenotypic characteristics of mature Bati, Borana and Short-eared Somali goats kept under traditional management systems so that the information generated can be used in designing breeding programs. The study was covered five districts in four administrative zones of Ethiopia representing Bati (in Oromia Zone) and Kalu (in South Wollo Zone) for Bati goats; Yabello (in Borana Zone) for Borana goats and Shinille and Erer (in Siti Zone) for Short-Eared Somali goats. A total of 345 households interview was made and phenotypic measurements were taken on 601(468 females and 133 males) heads of adult goats with 4 pair of permanent incisors (PPI). However, because of difficulty of finding adequate number of 4PPI sample males, measurements were taken from 2PPI and above males. In this study, in number, goats accounted for 72.01, 50.93 and 47.38% of other livestock species in Siti, around Bati and Borana, respectively. The least square mean (±SE) of goat flock size (44.02±3.33) per household in Siti was significantly (p0.05),whereas Short-eared Somali goats remained significantly (p<0.05) lower for most of the body measurement characteristics. The canonical analysis done on phenotypic measurements also put Bati and Borana goats closer by discriminating Short-eared Somali goat populations. The similarities between Bati and Borena goats and significance divergence of Short-eared Somali goats in phenotypic measurements suggested that the need of further molecular characterization study to validate information from phenotypic characterization. Correlation coefficient was consistently highest between live weight and chest girth in both sexes across the goat populations. Hence, linear measurements could be valuable to estimate live body weight for those farm communities where sensitive weighing scales are not readily available

Prevalence and associated risk factors of intestinal parasitic infections among children in pastoralist and agro-pastoralist communities in the Adadle woreda of the Somali Regional State of Ethiopia

BACKGROUND: Intestinal parasitic infections (IPIs) can cause illness, morbidity, and occasional mortality in children. Agro-pastoralist and pastoralist children in the Somali Regional State of Ethiopia (ESRS) are especially at risk for IPIs, as access to safe water, sanitation, and health services is lacking. Minimal data on the prevalence of IPIs and associated risk factors exists in this region. METHODOLOGY: We assessed the prevalence of IPIs and associated risk factors during the wet season from May-June 2021 in 366 children aged 2 to 5 years in four agro-pastoralist and four pastoralist kebeles (wards) in Adadle woreda (district) of the Shebelle zone, ESRS. Household information, anthropometric measurements, and stool samples were obtained from included children. Parasites were identified microscopically using Kato-Katz and direct smear methods. Risk factors were assessed using general estimating equation models accounting for clustering. PRINCIPAL FINDINGS: Overall prevalence of IPIs was 35%: 30.6% for single infections and 4.4% for poly-parasitic infections. Intestinal protozoan prevalence was 24.9%: 21.9% Giardia intestinalis, and 3.0% Entamoeba spp.. Intestinal helminth prevalence was 14.5%: 12.8% Ascaris lumbricoides, 1.4% hookworm (Ancylostoma duodenale /Necator americanus.), and 0.3% Hymenolepis nana. G. intestinalis infection was associated with drinking water sourced from the river (aOR 15.6, 95%CI 6.84, 35.4) and from collected rainwater (aOR 9.48, 95%CI 3.39, 26.5), with toilet sharing (aOR 2.93, 95%CI 1.36, 6.31) and with household ownership of cattle (1-5 cattle: aOR 1.65, 95%CI 1.13, 2.41; 6+ cattle: aOR 2.07, 95%CI 1.33, 3.21) and chickens (aOR 3.80, 95%CI 1.77, 8.17). A. lumbricoides infection was associated with children 36 to 47 months old (aOR 1.92, 95%CI 1.03, 3.58). CONCLUSIONS/SIGNIFICANCE: Improving access to safe water, sanitation, and hygiene services in Adadle and employing a One Health approach would likely improve the health of children living in (agro-) pastoralist communities in Adadle and the ESRS; however, further studies are required