Terahertz Magneto Optical Polarization Modulation Spectroscopy

We report the development of new terahertz techniques for rapidly measuring the complex Faraday angle in systems with broken time-reversal symmetry using the cyclotron resonance of a GaAs two-dimensional electron gas in a magnetic field as a system for demonstration of performance. We have made polarization modulation, high sensitivity (< 1 mrad) narrow band rotation measurements with a CW optically pumped molecular gas laser, and by combining the distinct advantages of terahertz (THz) time domain spectroscopy and polarization modulation techniques, we have demonstrated rapid broadband rotation measurements to < 5 mrad precision.Comment: 25 pages including 7 figures, introduces use of rotating polarizer with THz TDS for Complex Faraday Angle determinatio

Prolonged Application of High Fluid Shear to Chondrocytes Recapitulates Gene Expression Profiles Associated with Osteoarthritis

BACKGROUND: Excessive mechanical loading of articular cartilage producing hydrostatic stress, tensile strain and fluid flow leads to irreversible cartilage erosion and osteoarthritic (OA) disease. Since application of high fluid shear to chondrocytes recapitulates some of the earmarks of OA, we aimed to screen the gene expression profiles of shear-activated chondrocytes and assess potential similarities with OA chondrocytes. METHODOLOGY/PRINCIPAL FINDINGS: Using a cDNA microarray technology, we screened the differentially-regulated genes in human T/C-28a2 chondrocytes subjected to high fluid shear (20 dyn/cm(2)) for 48 h and 72 h relative to static controls. Confirmation of the expression patterns of select genes was obtained by qRT-PCR. Using significance analysis of microarrays with a 5% false discovery rate, 71 and 60 non-redundant transcripts were identified to be ≥2-fold up-regulated and ≤0.6-fold down-regulated, respectively, in sheared chondrocytes. Published data sets indicate that 42 of these genes, which are related to extracellular matrix/degradation, cell proliferation/differentiation, inflammation and cell survival/death, are differentially-regulated in OA chondrocytes. In view of the pivotal role of cyclooxygenase-2 (COX-2) in the pathogenesis and/or progression of OA in vivo and regulation of shear-induced inflammation and apoptosis in vitro, we identified a collection of genes that are either up- or down-regulated by shear-induced COX-2. COX-2 and L-prostaglandin D synthase (L-PGDS) induce reactive oxygen species production, and negatively regulate genes of the histone and cell cycle families, which may play a critical role in chondrocyte death. CONCLUSIONS/SIGNIFICANCE: Prolonged application of high fluid shear stress to chondrocytes recapitulates gene expression profiles associated with osteoarthritis. Our data suggest a potential link between exposure of chondrocytes/cartilage to abnormal mechanical loading and the pathogenesis/progression of OA

Neuroprotectants in stroke therapy

10.1517/14656566.9.6.887Expert Opinion on Pharmacotherapy96887-900EOPH

Twenty-Four Centrifuge Tests to Quantify Sensitivity of Lateral Spreading to Dr and PGA

Twenty-four centrifuge model tests have been conducted at nine different geotechnical centrifuge facilities around the world as part of the international LEAP effort (liquefaction experiments and analysis projects). All of the centrifuge models represent a 4 m deep 5 degree sloping submerged sand deposit. The mean effective PGA of the input motion for all of the experiments was approximately 0.15 g and the mean relative density was approximately 65%, but the effective PGA's varied between about 0.07 g and 0.3 g, and the relative densities varied between about 40% and 75%. The test matrix was designed to enable experimental quantification of not only the median response but also the trend and sensitivity of the model response to density and shaking intensity. Quantification of the sensitivity of the response to initial conditions is a prerequisite for objective evaluation of the quality of the model test data. In other words, a discrepancy between two experiments should be evaluated after accounting for the uncertainty in the initial conditions and the sensitivity of the response to initial conditions. For the first time, a sufficient number of experiments has been performed on a similar problem to provide meaningful quantitative evaluation of the trend between PGA, density, and displacement. The sensitivity is quantified by the gradient of the trend and the uncertainty of the trend is quantified from the residuals between the fitting data and the trend