Thermocouple installation in thin-walled tubes

Brazing process attaches thermocouples to slot in type-347 stainless steel tube flush around its circumference. Electrolytic flashing with gold enhances brazing process. This installation procedure does not significantly change calibration of thermocouples used and does not cause major wear spots in the thin-walled devices

Brorfelde Schmidt CCD Catalog (BSCC)

The Brorfelde Schmidt CCD Catalog (BSCC) contains about 13.7 million stars, north of +49 deg Declination with precise positions and V, R photometry. The catalog has been constructed from the reductions of 18,667 CCD frames observed with the Brorfelde Schmidt Telescope between 2000 and 2007. The Tycho-2 catalog was used for astrometric and photometric reference stars. Errors of individual positions are about 20 to 200 mas for stars in the R = 10 to 18 mag range. External comparisons with 2MASS and SDSS reveal possible small systematic errors in the BSCC of up to about 30 mas. The catalog is supplemented with J, H, and K_s magnitudes from the 2MASS catalog. The catalog data file (about 550 MB ASCII, compressed) will be made available at the Strasbourg Data Center (CDS).Comment: 16 pages, 22 figures, 2 tables, accepted by A

Stress Activation of a Bean Hydroxyproline-Rich Glycoprotein Promoter Is Superimposed on a Pattern of Tissue-Specific Developmental Expression

The HRGP4.1 gene, which encodes a cell wall hydroxyproline-rich glycoprotein, was isolated from a genomic library of bean (Phaseolus vulgaris L.). Two transcripts, one induced by wounding and one by elicitation, were transcribed from the same initiation site. The gene encodes a polypeptide of 580 amino acids with the amino terminal half consisting of repeats of the sequence serine-(proline)4-lysine-histidine-serine-(proline)4-(tyrosine)3-histidi ne and the carboxyl-terminal half composed of repeats of the sequence serine-(proline)4-valine-tyrosine-lysine-tyrosine-lysine. A 964-bp upstream promoter fragment was translationally fused to the beta-glucuronidase reporter gene (Escherichia coli uidA) and transferred into tobacco by Agrobacterium tumefaciens-mediated leaf disc transformation. Analysis of beta-glucuronidase activity showed that wounding caused local activation of the HRGP4.1 promoter in the phloem. Infection by tobacco mosaic virus was a less effective inducer than wounding. Stress induction was superimposed on tissue-specific developmental expression in stem nodes and root tips, suggesting that HRGP4.1 may have specific structural roles in development as well as protective functions in defense. Deletion analysis showed that control of tissue specificity and wound inducibility lies in a region between -94 and -251 relative to the transcription start site and that activation by infection lies outside that region

Delayed Toxicity Associated with Soluble Anthrax Toxin Receptor Decoy-Ig Fusion Protein Treatment

Soluble receptor decoy inhibitors, including receptor-immunogloubulin (Ig) fusion proteins, have shown promise as candidate anthrax toxin therapeutics. These agents act by binding to the receptor-interaction site on the protective antigen (PA) toxin subunit, thereby blocking toxin binding to cell surface receptors. Here we have made the surprising observation that co-administration of receptor decoy-Ig fusion proteins significantly delayed, but did not protect, rats challenged with anthrax lethal toxin. The delayed toxicity was associated with the in vivo assembly of a long-lived complex comprised of anthrax lethal toxin and the receptor decoy-Ig inhibitor. Intoxication in this system presumably results from the slow dissociation of the toxin complex from the inhibitor following their prolonged circulation. We conclude that while receptor decoy-Ig proteins represent promising candidates for the early treatment of B. anthracis infection, they may not be suitable for therapeutic use at later stages when fatal levels of toxin have already accumulated in the bloodstream

Polyploidization as a Retraction Force in Plant Genome Evolution: Sequence Rearrangements in Triticale

BACKGROUND: Polyploidization is a major evolutionary process in plants where hybridization and chromosome doubling induce enormous genomic stress and can generate genetic and epigenetic modifications. However, proper evaluation of DNA sequence restructuring events and the precise characterization of sequences involved are still sparse. METHODOLOGY/PRINCIPAL FINDINGS: Inter Retrotransposons Amplified Polymorphism (IRAP), Retrotransposons Microsatellite Amplified Polymorphism (REMAP) and Inter Simple Sequence Repeat (ISSR) largely confirmed the absence of any intraspecific variation in wheat, rye and triticale. The comparative analysis of banding profiles between wheat and rye inbred lines revealed 34% of monomorphic (common to both parental species) bands for the ten different primer combinations used. The analysis of triticale plants uncovered nearly 51% of rearranged bands in the polyploid, being the majority of these modifications, due to the loss of rye bands (83%). Sequence analysis of rye fragments absent in triticale revealed for instance homology with hydroxyproline-rich glycoproteins (HRGP), a protein that belongs to a major family of inducible defence response proteins. Conversely, a wheat-specific band absent in triticale comprises a nested structure of copia-like retrotransposons elements, namely Claudia and Barbara. Sequencing of a polyploid-specific band (absent in both parents) revealed a microsatellite related sequence. Cytological studies using Fluorescent In Situ Hybridization (FISH) with REMAP products revealed a widespread distribution of retrotransposon and/or microsatellite flanking sequences on rye chromosomes, with a preferential accumulation in heterochromatic sub-telomeric domains. CONCLUSIONS/SIGNIFICANCE: Here, we used PCR-based molecular marker techniques involving retrotransposons and microsatellites to uncover polyploidization induced genetic restructuring in triticale. Sequence analysis of rearranged genomic fragments either from rye or wheat origin showed these to be retrotransposon-related as well as coding sequences. Further FISH analysis revealed possible chromosome hotspots for sequence rearrangements. The role of chromatin condensation on the origin of genomic rearrangements mediated by polyploidization in triticale is also discussed

Modélisation de la qualité des stations et de la croissance individuelle des arbres dans des peuplements purs et mélangés de Pinus brutia dans le nord-est de la Grèce

International audienc

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead