14 research outputs found
Generation of radially polarized beam with a segmented spiral varying retarder
10.1364/OE.16.015694Optics Express162015694-1569
Thermo-responsive porous membranes of controllable porous morphology from triblock copolymers of polycaprolactone and poly(N-isopropylacrylamide) prepared by atom transfer radical polymerization
10.1021/bm7008922Biomacromolecules91331-339BOMA
Interpenetrating network hydrogels via simultaneous "click Chemistry" and atom transfer radical polymerization
10.1021/bm100268tBiomacromolecules1171810-1817BOMA
Synthesis, Characterization and Degradation Properties of Poly(α-angelica lactone-co-∊-caprolactone) Copolymers
Improving osteoblasts cells proliferation via femtosecond laser surface modification of 3D-printed poly-ε-caprolactone scaffolds for bone tissue engineering applications
The role of crystallinity on differential attachment/proliferation of osteoblasts and fibroblasts on poly (caprolactone-co-glycolide) polymeric surfaces
3D
© 2021 Wiley Periodicals LLC.Fabrication of scaffolds using polymers and then cell seeding is a routine protocol of tissue engineering applications. Synthetic polymers have adequate mechanical properties to substitute for some bone tissue, but they are generally hydrophobic and have no specific cell recognition sites, which leads to poor cell affinity and adhesion. Some natural polymers, have high cell affinity but are mechanically weak and do not have the strength required as a bone supporting material. In the present study, 3D printed hybrid scaffolds were fabricated using PCL and GelMA carrying dental pulp stem cells (DPSCs), which is printed in the gaps between the PCL struts. This cell loaded GelMA was shown to support osteoinductivity, while the PCL provided mechanical strength needed to mimic the bone tissue. 3D printed PCL/GelMA and GelMA scaffolds were highly stable during 21 days of incubation in PBS. The compressive moduli of the hybrid scaffolds were in the range of the compressive moduli of trabecular bone. DPSCs were homogeneously distributed throughout the entire hydrogel component and exhibited high cell viability in both scaffolds during 21 days of incubation. Upon osteogenic differentiation DPSCs expressed two key matrix proteins, osteopontin and osteocalcin. Alizarin red staining showed mineralized nodules, which demonstrates osteogenic differentiation of DPSCs within GelMA. This construct yielded a very high cell viability, osteogenic differentiation and mineralization comparable to cell culture without compromising mechanical strength suitable for bone tissue engineering applications. Thus, 3D printed, cell loaded PCL/GelMA hybrid scaffolds have a great potential for use in bone tissue engineering applications