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3D
Authors
Abouzeid RE
Anada T
+79 more
Aubin JE
Bahcecioglu G
Bao C
Bektas CK
Bektas CK
Bertassoni LE
Bruyas A
Buyuksungur S
Celikkin N
Celikkin N
Choi JR
Cunniffe GM
Dong L
Duarte Campos DF
Eke G
Entezari A
Erdem A
Freeman FE
Gao G
García‐Gareta E
Groll J
Hasirci N
Heo EY
Hoch E
Isikli C
Jian Z
Kang H
Khanna‐Jain R
Kim MH
Kong B
Koo Y
Lee BH
Lee D
Lin CH
Lin K
Liu F
Luo L
Malikmammadov A
Malikmammadov E
Malikmammadov E
Meena LK
Nabavi MH
Nakamura A
Nguyen BNB
Park J
Park JH
Park JY
Posritong S
Qiao Y
Ramis JM
Ren B
Rodas‐Junco BA
Roosa SMM
Rothrauff BB
Ruiz‐Cantu L
Saekhor K
Sawyer SW
Schuurman W
Sezer UA
Shao H
Shim JH
Shim JH
Short AR
Tamay DG
Tiaw K
Tondera C
Turnbull G
Visser J
Wang W
Wang X
Woodruff MA
Wu W
Xiao W
Yang X
Yue K
Zamani Y
Zhao C
Zhao D
Zhou X
Publication date
1 January 2021
Publisher
'Wiley'
Doi
Cite
Abstract
© 2021 Wiley Periodicals LLC.Fabrication of scaffolds using polymers and then cell seeding is a routine protocol of tissue engineering applications. Synthetic polymers have adequate mechanical properties to substitute for some bone tissue, but they are generally hydrophobic and have no specific cell recognition sites, which leads to poor cell affinity and adhesion. Some natural polymers, have high cell affinity but are mechanically weak and do not have the strength required as a bone supporting material. In the present study, 3D printed hybrid scaffolds were fabricated using PCL and GelMA carrying dental pulp stem cells (DPSCs), which is printed in the gaps between the PCL struts. This cell loaded GelMA was shown to support osteoinductivity, while the PCL provided mechanical strength needed to mimic the bone tissue. 3D printed PCL/GelMA and GelMA scaffolds were highly stable during 21 days of incubation in PBS. The compressive moduli of the hybrid scaffolds were in the range of the compressive moduli of trabecular bone. DPSCs were homogeneously distributed throughout the entire hydrogel component and exhibited high cell viability in both scaffolds during 21 days of incubation. Upon osteogenic differentiation DPSCs expressed two key matrix proteins, osteopontin and osteocalcin. Alizarin red staining showed mineralized nodules, which demonstrates osteogenic differentiation of DPSCs within GelMA. This construct yielded a very high cell viability, osteogenic differentiation and mineralization comparable to cell culture without compromising mechanical strength suitable for bone tissue engineering applications. Thus, 3D printed, cell loaded PCL/GelMA hybrid scaffolds have a great potential for use in bone tissue engineering applications
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Last time updated on 28/05/2021
OpenMETU (Middle East Technical University)
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oai:https://open.metu.edu.tr:1...
Last time updated on 02/12/2021