Alterations in PGC1[alfa] expression levels are involved in colorectal cancer risk: a qualitative systematic review

Background: Colorectal cancer (CRC) is a major global public health problem and the second leading cause of cancer-related death. Mitochondrial dysfunction has long been suspected to be involved in this type of tumorigenesis, as supported by an accumulating body of research evidence. However, little is known about how mitochondrial alterations contribute to tumorigenesis. Mitochondrial biogenesis is a fundamental cellular process required to maintain functional mitochondria and as an adaptive mechanism in response to changing energy requirements. Mitochondrial biogenesis is regulated by peroxisome proliferator-activated receptor gamma coactivator 1-? (PPARGC1A or PGC1?). In this paper, we report a systematic review to summarize current evidence on the role of PGC1? in the initiation and progression of CRC. The aim is to provide a basis for more comprehensive research. Methods: The literature search, data extraction and quality assessment were performed according to the document Guidance on the Conduct of Narrative Synthesis in Systematic Reviews and the PRISMA declaration. Results: The studies included in this review aimed to evaluate whether increased or decreased PGC1? expression affects the development of CRC. Each article proposes a possible molecular mechanism of action and we create two concept maps. Conclusion: Our systematic review indicates that altered expression of PGC1? modifies CRC risk. Most studies showed that overexpression of this gene increases CRC risk, while some studies indicated that lower than normal expression levels could increase CRC risk. Thus, various authors propose PGC1? as a good candidate molecular target for cancer therapy. Reducing expression of this gene could help to reduce risk or progression of CRC

Phase structure and heating efficiency of as-synthesised iron-oxide nanoparticles and after long time storage in glycerol

In the present work the structural characteristics and heating efficiencies of iron-oxide nanoparticles were studied. The investigated samples were produced by co-precipitation of Fe-O nanoparticles from mixture of Fe2+ and Fe3+ salts in the alkaline environment. Furthermore, the influence of processing route and storage environment on the phase structure and heating efficiency was studies for glycol dispersed nanoparticles. The heating efficiency experiments were performed on 5 wt% glycerol dispersions of Fe-O nanoparticles. The measurements were carried out on specimens directly after precipitation and for those kept in glycerol for substantial period of time in order to determine how the storage environment can impact their performance as a heating medium. Furthermore, comparative studies for nanoparticles produced at different processing routes were performed. The structural studies were carried out using X-ray diffractometry and transmission electron microscopy. The heating efficiencies and specific loss powers were determined using the self-constructed setup operating in the alternating magnetic field of various amplitudes from 2 to 4 kA/m and frequency of 100 kHz

Magneto-responsive hyaluronan hydrogel for hyperthermia and bioprinting: Magnetic, rheological properties and biocompatibility

Magneto-responsive soft hydrogels are used for a number of biomedical applications, e.g., magnetic hyperthermia, drug delivery, tissue engineering, and neuromodulation. In this work, this type of hydrogel has been fabricated from hyaluronan (HA) filled with a binary system of Al2O3 nanoparticles and multicore magnetic particles (MCPs), which were obtained by clustering of superparamagnetic iron oxide FeOx NPs. It was established that the presence of diamagnetic Al2O3 has several positive effects: it enhances the hydrogel storage modulus and long-term stability in the cell cultivation medium; prevents the magnetic interaction among the MCPs. The HA hydrogel provides rapid heating of 0.3 °C per min under exposure to low amplitude radio frequency alternating magnetic field. Furthermore, the magneto-responsive hydrogel was successfully used to encapsulate cells and extrusion-based 3D printing with 87±6% cell viability, thus providing a bio-ink. The combination of high heating efficiency, softness, cytocompatibility, and 3D printability of magnetic HA hydrogel leads to a material suitable for biomedical applications