HLA-G: expression in human keratinocytes in vitro and in human skin in vivo

Classical, polymorphic major histocompatibility complex class I molecules are expressed on most nucleated cells.They present peptides at the cell surface and, thus, enable the immune system to scan peptides for their antigenicity. The function of the other, nonclassical class I molecules in man is controversial. HLA-G which has been shown by transfection experiments to be expressed at the cell surface, is only transcribed in placental tissue and in the fetal eye.Therefore, a role of HLA-G in the control of rejection of the allogeneic fetus has been discussed. We found that HLA-G expression is induced in keratinocytes by culture in vitro. Three different alternative splicing products of HLA-G can be detected: a full length transcript, an mRNA lacking exon 3 and a transcript devoid of exon 3 and 4. Reverse transcription followed by polymerase chain reaction also revealed the presence of HLA-G mRNA in vivo in biopsies of either diseased or healthy skin

Evaluation of a Direct Fed Microbial an an Enzymatically Hydrolyzed Yeast Product Fed Alone or in Combination to Beef Steers Administered Ractopamine Hydrochloride 28 Days Prior to Harvest During Summer Months in the Northern Plains

Study Description: Single-sourced, newly weaned steers (n=256; initial BW=542 ± 3.7lb; n=64 steers/treatment; 8 steers/pen) were blocked by location in a 2×2 factorial arrangement of DFM (Certillus CP B1801 Dry; Bacillus subtilis, Lactobacillus plantarum; 28 g/steer·d-1) and YCW (Celmanax; 18 g/steer·d-1). Temperature-humidity index (THI) was calculated as: THI=0.81×ambient temperature+[relative humidity×(ambient temperature-14.40)]+46.40. On d-1 and 2 and d-21 and 22 on RH, respiration rate (RR) and panting scores (PS) were determined before and after AM and PM feedings (0700h, 1100h, 1400h, 1700h). RR (n=3 steers/pen) was calculated from: 600/seconds required for 10 flank movements. PS utilized this scoring system: 0 (not distressed) to 4.5 (severely distressed)

Evaluation of a Direct Fed Microbial and/or an Enzymatically Hydrolyzed Yeast Product in Diets Containing Monensin Sodium on Feedlot Phase Growth Performance, Efficiency of Dietary Net Energy Utilization, and Carcass Characteristics in Newly Weaned Beef Steers Fed in Confinement for 258 Days

Study Description: Single-sourced, newly weaned steers (n = 256; initial body weight (BW) = 542 ± 3.7 lb) were allotted to 32 pens (n = 8 pens/treatment with 8 steers/pen). Steers were blocked by location in a 2x2 factorial treatment arrangement of DFM (Certillus CP B1801 Dry; Bacillus subtilis, Lactobacillus plantarum; 28 g/steer·d-1) and YCW (Celmanax; 18 g/steer·d-1). Steers were vaccinated and poured at processing and individually weighed on d 1, 14, 42 (end of receiving phase; implanted), 77, 105 (end of growing phase), 133, 161 (implanted), 182, 230 (start ractopamine HCl) and 258. Growth performance and carcass measurements were recorded

The object sculpture

Wilson co-curated an international survey exhibition of 30 sculptures past and present, each work by a different artist. Works were installed throughout the Henry Moore Institute, from the front steps to the library, the final work being the catalogue, designed as a piece of work by artist Pae White. An exhibition arising out of a unique set of curatorial conditions – the entire budget and apparatuses of the museum were handed over to three artists seen, through their exhibited work, to have manifested a particular interest in the condition of sculpture today. Together, they were set the task of answering the question ‘What is Sculpture?’ Over the following 18 months, through extensive meetings and correspondences, an exhibition was made which took the full extent of the Institute as its frame, from the library to the front steps. The artists employed an independent researcher to gather information surrounding each chosen work, and offered all this archival material to the sculptor Pae White who was asked to produce a catalogue as the final artwork for exhibition. The collection of works and texts on display articulated the shared international and historical context in which the artists agreed they now work. But the exhibition also announced its own particular modus operandi – each discrete artwork was carefully installed according to its own needs, as well as being there as representative of its class of sculpture. Visitors were invited to encounter discrete works in the first instance, divorced from their contexts, and this prioritising of direct experience was reflected in the invitation cards, where the list of works took precedence over the list of artists. The catalogue, as well as being an artwork in its own right, provided factual material surrounding each exhibited piece with a minimum of interpretation, offering the first step in helping visitors piece together a more contextual reading. Wilson took full equal part in the curatorial process up to final installation of the works. The exhibition was widely reviewed and has an international reputation - it helped set the terms for discussing contemporary sculptural practice

HEROS - Sounding Rocket Development by the HyEnD Project

The student team Hybrid Engine Development (HyEnD) of the University of Stuttgart is taking part with the Institute of Space Systems (IRS) in the DLR educational program STERN (Studentische Experimentalraketen). This program supports students at German universities to design, build and launch an experimental rocket within 3 years. HyEnD is developing a hybrid rocket called HEROS (Hybrid Experimental Rocket Stuttgart) with a thrust of 10 kN, a total impulse of over 100 kNs and an expected lift off weight up to 175 kg. HEROS is aimed to be launched in October 2015 from Esrange in Sweden to an expected flight altitude of 40 to 50 km. The current altitude record for amateur rockets in Europe is at approximately 12.5 km. HEROS’ hybrid rocket engine HyRES uses a paraffin-based solid fuel and nitrous oxide as the liquid oxidizer. In the course of the STERN program the student team not only has to master the difficulties of a high thrust hybrid rocket engine but also the development of a light weight oxidizer tank, valves, rocket structure, recovery system and electronics. Concluding, the development and construction of a lightweight hybrid sounding rocket for the given requirements and budget is described herein