Municipal Solid Waste Characterisation and Quantification as a measure towards Effective Waste Management in the Takoradi Sub-Metro, Ghana

Waste management is a major challenge for many metropolitan and municipal assemblies in Ghana. The quantity of waste generated from the cities keeps increasing at a faster rate without increasing facilities to match its management. In the Takoradi sub-metro for instance, all the waste generated ends up at the final disposal sites without any recovery of the valuables. Proper management of the generated waste requires reliable and informative data which could assist in the collection as well as value addition process. Waste separation efficiency; willingness to separate waste at source; physical composition and per capita waste generated by households within the Takoradi sub-metropolis were studied over a five-week period. Questionnaire, interviews and survey were employed in the collection of the required data. The data were analysed using SPSS. The results showed solid waste composition of 60.0% organics, 11.5% plastics, 8.0% inert materials, 7.1% papers and cardboard, 5.0% miscellaneous materials, 2.9% textiles, 2.4% metals, 1.5% glasses and 1.2% leather and rubber. Over 80% of the waste fraction has the potential for recovery into other products; with this, 22.7% could be recycled and 63.6% suitable for biological conversions such as composting and anaerobic digestion since they have a moisture content as high as 55%. The average per capita waste generated within the sub-metro was 0.70 kg/cap/day. Households were able to separate the organic fractions from the rest of the waste fractions reaching effectiveness of 92% for organic separation and 83% for all other wastes. The data generated on the quantity and composition of the waste stream in the Metropolis would play a positive role in solid waste management and help solid waste managers make informed decisions on waste management options.     Keywords: Waste Sorting and Separation, Biodegradables, Household Waste, Separation Efficienc

Physico-chemical Assessment of Mosquito Breeding Sites from Selected Mining Communities at the Obuasi Municipality in Ghana.

A survey was conducted in the Obuasi Municipality in Ghana to assess the impact of some physic-chemicals in waters of mosquito breeding sites from 15 randomly selected communities. The water samples, collected fortnightly for eight months, were analyzed using spectrophotometry and other standard laboratory protocols at the AngloGold Ashanti Environmental Quality Assurance Laboratory for temperature, total suspended solids, total dissolved solids, dissolved oxygen, electrical conductivity and pH. The physico-chemical parameters which ranged from 17.03 ± 0.18?C - 24.06 ± 0.18?C (temperature), 17.03 ± 4.04mg/L - 96.67 ± 4.04mg/L (TSS), 1.09±3.23mg/L - 35.67±3.23mg/L (TDS), 3.97 ± 0.13mg/L - 7.43 ± 0.13mg/L (DO), 17.00 ± 1.30µScm-3 - 83.00 ± 1.30 µScm-3 (EC) and 7.77 ± 0.0 - 10.70 ± 0.01 (pH) were much lower relative to the EPA Maximum Permissible Limits. It is apparent that under rising temperature conditions of climate change, the mosquito’s habitat may be highly favoured for adaptation and prolific breeding in the tropics and this further creates the opportunity for research partners to get actively involved in finding integrated control measures to counteract the life cycle of the pest. Keywords: Anglogold Ashanti, Obuasi Municipality, Physio-chemical Analysis, Mosquito Breeding Water

Microbiological quality and metal levels in wells and boreholes water in some peri-urban communities in Kumasi, Ghana

Many communities in Kumasi, Ghana, are increasingly dependent on boreholes and hand dug wells. The aim of this study was to examine the drinking water suitability of 6 wells and 3 boreholes in periurban communities in Kumasi, between December 2003 and January 2005. Total coliforms, faecal coliforms and enterococci were enumerated using the standard most probable number method and membrane filtration methods. The heavy metals in the water samples were determined using the atomic absorption spectrometry method. Overall, significantly higher bacterial counts were recorded during the wet (rainy) season compared to the dry (harmattan) season. Faecal coliforms counts (FCC) in 3 borehole samples ranged between 3 x 101 and 3.5 x 107 per 100 ml (geometric means 1.82, 1.75 and 2.8 x 104) while mean numbers of enterococci were 103-105 times lower. The range and geometric means of FCC was similar in samples from wells but levels of enterococci were 8 times higher than in boreholes. Manganese and iron levels were well within the WHO standards for all 9 sites but lead levels except for one site (Boadi) were all higher than the WHO standard. A brief sanitation survey at each site suggested that wells and boreholes were frequently cited near latrines, refuse tips and other social amenities, and in the vicinity of domestic or grazing animals. In Kumasi, the water from shallow wells and boreholes, upon which the local communities depend is of poor quality. The data are being used to advise the local government. An integrated approach is required to minimise faecal pollution of wells and boreholes within peri-urban communities.Key words: Boreholes, enterococci, faecal coliforms, peri-urban communities, total coliforms, wells

Assessment of contamination of singed hides from cattle and goats by heavy metals in Ghana

Many local butchers in Ghana have been using scrap tyres as substitute for firewood to singe slaughtered ruminants. This study analysed such singed hides from seven goats and three cattle in unregulated local slaughtering operations near the Kumasi abattoir, for risk of heavy metal contamination (Mg, Mn, Cu, Ni, Cd, Pb and Zn) using atomic absorption spectrometry. Relative to unsinged control hides, singed treatments generally elevated heavy metal levels in both goats and cattle hides. Magnesium, Mn, Cu, Ni and Cd concentrations in un-singed goats hide increased by 23, 29, 54, 24 and 20% respectively to 108.63 ± 3.85, 0.83 ± 0.13, 1.63 ± 0.13, 2.15 ± 0.29 and 2.26 ± 0.20 mg/kg in the singed material. Similarly, singeing increased Mg, Mn, Cu, Ni, Cd and Zn concentrations in cattle hide by 94, 49, 130, 33, 174 and 1055% to 80.0 ± 5.25, 2.13 ± 0.26, 5.67 ± 1.24, 3.50 ± 0.17, 4.20 ± 0.17, and 204.49 ± 36.69 mg/kg respectively. Lead concentration in cattle hide on the other hand decreased by 34% upon singeing, from 4.61 ± 0.30 mg/kg in un-singed controls to 3.06 ± 0.26 mg/kg in singed material. High concentrations of the metals in singed hides could not be entirely attributed to the singed treatment alone, in view of considerable background levels of heavy metals recorded in the un-singed samples; a situation that probably reflected a larger problem of heavy metal pollution in the local environment. Concentrations of Cd and Pb in hides were above maximum permissible levels (MPLs) of 0.05 and 0.01 mg/kg respectively set by the European Commission Regulation, whilst Zn was also above MPL of 50.0 mg/kg, according to the Meat Food Products Order of India. It was concluded that the hides were potentially unsafe for human consumption.Key words: Heavy metal contamination, meat products, singed treatments, consumed animal hide

Traced Metals and Nutrients in Mosquito Breeding Sites at the Obuasi Municipality of Ashanti Region in Ghana

A survey was conducted in the Obuasi Municipality to assess the impact of some trace metals and nutrients on mosquito breeding sites from 15 randomly selected communities. The water samples, collected fortnightly for eight months, were analyzed using spectrophotometry and other standard laboratory protocols at the AngloGold Ashanti Environmental Quality Assurance Laboratory. The results on trace metals, and nutrients did not reveal any significant pattern of attendant pollution that would influence the breeding patterns of mosquitoes. However, the trace metals ranged from 0.12±0.01mg/L - 13.42±0.01mg/L and 0.01±0.01mg/L - 0.30±0.01mg/L for Fe and Pb respectively in the waters whilst As and Zn were not detected. For the nutrients - nitrate values which were > 1 ranged from 2mg/L – > 40mg/L. Sulphate levels varied between < 20mg/L and > 200mg/L and were above the EPA Maximun Permissible Limit of 1.5 mg/L  for polluted  natural water bodies. There was no definite pattern in the concentrations of phosphates which varied between 0.1mg/L and 1.5mg/L in the waters. It is apparent that under rising temperature conditions of climate change, the mosquito’s habitat may be highly favoured for adaptation and prolific breeding in the tropics and this further creates the opportunity for research partners to get actively involved in finding integrated control measures to counteract the life cycle of the pest. Keywords: Anglogold Ashanti, Obuasi Municipality, Traced Metal Analysis, Mosquito Breeding Water

Groundwater quality, sanitation and vulnerable groups: case study of Bawku East District

The effect of human hygienic behaviour on the quality of groundwater was investigated using standard methods for trace elements and bacteria pollution indicators in Bawku East District of Ghana in 2006. No iron was detected in water; manganese levels were higher in wells than boreholes and fluoride levels exceeded the WHO standard (1.5mg/l) by 0.5-1.0mg/l in one well and a borehole at Bawku. Typical bacteria numbers deviated from normal trends (i.e.1014, 1011, 108, and 104 for total coliforms, faecal coliforms, E. coli and enterococci in the well water compared to 106, 105, 103 and 102 in the boreholes respectively). The six UNICEF boreholes were within the WHO guideline of zero helminthes (100-1 ml) but the wells were not. Hence, quality of the boreholes was higher than the wells for drinking purposes

Investigation of yeast genes possibly involved in mtDNA stability using the nematode Caenorhabditis elegans

Screening of Caenorhabditis elegans genes possibly involved in the mitochondrial genome maintenance was performed using our previous validated method of RNAi combined with ethidium bromide. This was to knock down C. elegans genes homologous to yeast genes known to be involved in mtDNA stability but of unknown molecular function or to identify transient components that could play important role on the stability of mtDNA in a temporal and/or spatial manner. C. elegans homologs for 11 genes among 27 yeast genes for which deletion leads to a rho0 state were found, however, only 5 genes were present in the RNAi library. Out of these 5 genes, 1 gene (homolog of GEM1) gave a clear L3 arrest on RNAi and ethidium bromide indicating its involvement on mtDNA stability. Four other genes homologs of MTG2, YER087W, AVL9 and RRG3 did not lead to L3 arrest even though their deletion in Saccharomyces cerevisiae leads to rho0 state. Although MTG2 has been reported to be important in the function and structure on mtDNA stability in yeast, our results did not support those findings in C. elegans. The human homolog of this gene (MIRO1) can be considered as a candidate gene involved in mtDNA stability and sequenced in patients with mtDNA depletion diseases.Keywords: mtDNA, Caenorhabditis elegans, nucleoid, RNAi, candidate genes, homolog, MIRO

Pointed gaps in the provision, quality, patronage and management of toilet facilities in Bawku East District

The objective was to assess the level of sanitation and hygiene with baseline information on the impact of onsite sanitation facility development on public health in the Bawku East District using hundred and sixty eight (n=168) respondents from five randomly selected communities according to UNICEF water and sanitation support programs for a survey. Public latrine alone accounted for 43% patronage of toilet facilities in the district. Unfortunately, about 70% of the population who preferred household toilets rather patronized open defecation due to poverty and inadequate good toilet facilities at user ratio of 1: over 500 people with very low seat capacities. Among the subjects encountered, 42% were unskilled labourers whilst 27% students, who, could hardly afford the comfort of household toilet facilities

Epidemiology of cervical human papillomavirus (HPV) infection and squamous intraepithelial lesions (SIL) among a cohort of HIV-infected and uninfected Ghanaian women.

BACKGROUND: There is limited data in Ghana on the epidemiology of HPV and cervical neoplasia and their associations with HIV. This study aimed to compare among HIV-1 seropositive and HIV-seronegative Ghanaian women: (1) the prevalence, genotype distribution and risk factors associated with cervical HPV infection; and (2) the prevalence and risk factors associated with abnormal cervical cytology. METHODS: A comparative frequency-matched study was conducted in a systematic sample of women aged ≥18 years attending HIV and general outpatient clinics in Cape Coast Teaching Hospital, Ghana. Participants were interviewed and cervical samples collected for HPV genotyping (Seegene Anyplex-II HPV28) and cytological testing. RESULTS: Overall, 333 women were recruited, 163 HIV-1 seropositive and 170 HIV-seronegative women of mean age 43.8 years (SD ±9.4)) and 44.3 years (SD ±12.8), respectively. The prevalence of 14 high-risk (hr) HPV genotypes was higher among HIV-1 seropositive women (65.6% vs. 30.2%, P < 0.0001), as was proportion with multiple hr.-HPV infections (60.6% vs. 21.3%, P < 0.0001). HPV35 was the most prevalent hr.-HPV genotype in both groups (11.9% and 5.3%). The main factors associated with hr.-HPV infection were age for HIV-positive women and circumcision status of main sexual partner for both HIV-negative and positive women. Abnormal cervical cytology prevalence was higher among HIV-1 seropositive women (any SIL: 14.1% vs. 1.2%, P < 0.0001; low-grade SIL [LSIL]: 4.9% vs. 0.6%, P = 0.02; high-grade SIL: 1.8% vs. 0%, P = 0.07). Among HIV-1 seropositive women, number of pregnancies and CD4+ cell count were associated with LSIL+ cytology. There was strong association between LSIL+ abnormalities and HPV35 (aOR = 4.7, 95%CI: 1.3-17.7, P = 0.02). CONCLUSIONS: HIV-1 infected women bear significant burden of HPV infection and related disease. Prevention and screening programmes should be specifically deployed for this population in Ghana

Estimating malaria transmission risk through surveillance of human–vector interactions in northern Ghana

Background: Vector bionomics are important aspects of vector-borne disease control programs. Mosquito-biting risks are affected by environmental, mosquito behavior and human factors, which are important for assessing exposure risk and intervention impacts. This study estimated malaria transmission risk based on vector–human interactions in northern Ghana, where indoor residual spraying (IRS) and insecticide-treated nets (ITNs) have been deployed. Methods: Indoor and outdoor human biting rates (HBRs) were measured using monthly human landing catches (HLCs) from June 2017 to April 2019. Mosquitoes collected were identified to species level, and Anopheles gambiae sensu lato (An. gambiae s.l.) samples were examined for parity and infectivity. The HBRs were adjusted using mosquito parity and human behavioral observations. Results: Anopheles gambiae was the main vector species in the IRS (81%) and control (83%) communities. Indoor and outdoor HBRs were similar in both the IRS intervention (10.6 vs. 11.3 bites per person per night [b/p/n]; z = −0.33, P = 0.745) and control communities (18.8 vs. 16.4 b/p/n; z = 1.57, P = 0.115). The mean proportion of parous An. gambiae s.l. was lower in IRS communities (44.6%) than in control communities (71.7%). After adjusting for human behavior observations and parity, the combined effect of IRS and ITN utilization (IRS: 37.8%; control: 57.3%) on reducing malaria transmission risk was 58% in IRS + ITN communities and 27% in control communities with ITNs alone (z = −4.07, P < 0.001). However, this also revealed that about 41% and 31% of outdoor adjusted bites in IRS and control communities respectively, occurred before bed time (10:00 pm). The mean directly measured annual entomologic inoculation rates (EIRs) during the study were 6.1 infective bites per person per year (ib/p/yr) for IRS communities and 16.3 ib/p/yr for control communities. After considering vector survival and observed human behavior, the estimated EIR for IRS communities was 1.8 ib/p/yr, which represents about a 70% overestimation of risk compared to the directly measured EIR; for control communities, it was 13.6 ib/p/yr (16% overestimation). Conclusion: Indoor residual spraying significantly impacted entomological indicators of malaria transmission. The results of this study indicate that vector bionomics alone do not provide an accurate assessment of malaria transmission exposure risk. By accounting for human behavior parameters, we found that high coverage of ITNs alone had less impact on malaria transmission indices than combining ITNs with IRS, likely due to observed low net use. Reinforcing effective communication for behavioral change in net use and IRS could further reduce malaria transmission