TOWARDS CATALYTIC OXIDATIVE DEPOLYMERIZATION OF LIGNIN

Lignin is one of the most abundant and underutilized biopolymers on earth. Primarily composed on three monolignol units (sinapyl, coniferyl, and p-coumaryl alcohol), lignin is formed through a radical pathway resulting in an assortment of linkages, of which the β-O-4 linkage is the most prevalent (up to 60% in some hardwood species). In planta, lignin plays an important role in water transport and in protecting plants from chemical and biological attack. Traditional attempts to depolymerize lignin have focused on the cleavage of β-O-4 linkages via thermal or reductive routes. However these pathways lead to low-value, unstable product mixtures. Moreover, typical product yields are low and the highly corrosive reaction medium results in added expense. More recently, catalytic oxidations have been studied as a viable means to lignin utilization. The present work will review the state-of-the-art of lignin oxidations, and focus on stoichiometric and catalytic attempts to oxidize lignin and lignin model compounds in order achieve selective stepwise depolymerization of lignin. Specifically, activated dimethyl sulfoxides and LDH catalysts were evaluated for lignin and/or lignin model compound oxidations leading, in some cases, to unexpected products

Pre- and post-processing for Cosmic/NASTRAN on personal computers and mainframes

An interface between Cosmic/NASTRAN and GIFTS has recently been released, combining the powerful pre- and post-processing capabilities of GIFTS with Cosmic/NASTRAN's analysis capabilities. The interface operates on a wide range of computers, even linking Cosmic/NASTRAN and GIFTS when the two are on different computers. GIFTS offers a wide range of elements for use in model construction, each translated by the interface into the nearest Cosmic/NASTRAN equivalent; and the options of automatic or interactive modelling and loading in GIFTS make pre-processing easy and effective. The interface itself includes the programs GFTCOS, which creates the Cosmic/NASTRAN input deck (and, if desired, control deck) from the GIFTS Unified Data Base, COSGFT, which translates the displacements from the Cosmic/NASTRAN analysis back into GIFTS; and HOSTR, which handles stress computations for a few higher-order elements available in the interface, but not supported by the GIFTS processor STRESS. Finally, the versatile display options in GIFTS post-processing allow the user to examine the analysis results through an especially wide range of capabilities, including such possibilities as creating composite loading cases, plotting in color and animating the analysis

Effects of isometric exercise on the relationship between muscle pain and resting blood pressure and resting heart rate

Title from PDF of title page (University of Missouri--Columbia, viewed on Aug. 18, 2010).The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.Thesis advisor: Dr. Pamela Hinton.M.A. University of Missouri--Columbia 2010.Silent myocardial ischemia is a common phenomenon in patients with coronary heart disease (CHD). This may be related to findings that high BP is associated with suppression of chest pain during episodes of myocardial ischemia. Examination of the relationship between pain and cardiovascular responses during and following resistance exercise may provide insight in the phenomenon of silent ischemia. Purpose: The primary objective of this study was to examine the relationship between isometric exercise-induced muscle pain and resting blood pressure (BP) and heart rate (HR). Five exploratory objectives related to the effects of eccentric exercise pre- and two days post-exercise were also examined: 1. To examine differences in the strength of the relationship between isometric exercise-induced muscle pain ratings and resting BP and HR. 2. To examine the change in salivary cortisol. 3. To examine the differences in the strength of the relationship between non-exercising extension pain and resting BP and HR. 4. To examine the differences in the strength of the relationship between resting salivary cortisol and resting BP and HR. 5. To examine if the change in resting salivary cortisol was significantly correlated to the change in non-exercising extension pain. Methods: Thirty-two participated this randomized controlled trial, which included two exercise sessions. In the first session baseline measurements of blood pressure, HR, cortisol and pain ratings were taken. Tonic and phasic isometric muscle contraction tests were performed following all of the baseline measurements. After the isometric muscle contraction tests, participants (n=16) performed three sets of 12 eccentric muscle contractions. All of the measurements and isometric muscle contraction tests were completed again, two days post-eccentric exercise. Results: Isometric exercise-induced pain and resting BP and HR was not found to be significantly or negatively correlated. None of the relationships examined in the exploratory objectives were significant from pre- to posteccentric exercise. Salivary cortisol concentration did not significantly change pre- to posteccentric exercise. Conclusion: A negative relationship between exercise-induced pain and resting BP and HR was not detected, which may be due to several factors, such as the type of pain induced during exercise or the timing of the BP and HR measurement.Includes bibliographical reference

Fragmented whole : a theory of narrative and identity

Title from PDF of title page (University of Missouri--Columbia, viewed on August 23, 2010).The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.Dissertation advisor: Dr. Loreen N. Olson.Vita.Ph. D. University of Missouri--Columbia 2010.The act of storytelling is one way to understand the complex nature of identity. Through the act of storytelling, human communication is enacted in the world, and the performance of the story is an act of identification of the self. Like a kaleidoscope image that changes depending on the way it is turned and the amount of light it absorbs, so too, do narratives of identity change depending on the stories being assimilated and the context within which the assimilation takes place. The purpose of this study is to conceptualize identity with narration as a central component of identity formation and performance, leading to a new theory called narrative of identity.Includes bibliographical reference

Phase function effects on oceanic light fields

Numerical simulations show that underwater radiances, irradiances, and reflectances are sensitive to the shape of the scattering phase function at intermediate and large scattering angles, although the exact shape of the phase function in the backscatter directions (for a given backscatter fraction) is not critical if errors of the order of 10% are acceptable. We present an algorithm for generating depth–and wavelength-dependent Fournier–Forand phase functions having any desired backscatter fraction. Modeling of a comprehensive data set of measured inherent optical properties and radiometric variables shows that use of phase functions with the correct backscatter fraction and overall shape is crucial to achieve model–data closure

ANALYSIS OF β8 INTEGRIN IN NEUROGENESIS AND NEUROVASCULAR HOMEOSTASIS

Neurogenesis in the adult mouse brain occurs within the subventricular zone (SVZ) of the lateral ventricle. In the SVZ, neural stem cells (NSC) reside in a specialized microenvironment, or vascular niche, consisting of blood vessels and their basement membranes. Most NSCs in the SVZ differentiate into progenitor cells, which further differentiate to generate neuroblasts, which then migrate from the SVZ to the olfactory bulbs (OB) along the rostral migratory stream (RMS). ECM-mediated adhesion and signaling within the vascular niche likely contribute to proper NSC self-renewal, survival, differentiation and neuroblast motility. The mechanisms that control these events are poorly understood. Previous studies from our group and others have shown that loss of the ECM receptor, αvβ8 integrin, in NSCs in the embryonic mouse brain leads to severe developmental vascular defects and premature death. Here, the functions of αvβ8 integrin in the adult brain have been examined using mice that have been genetically manipulated to lack a functional β8 integrin gene. This study reveals that loss of β8 integrin leads to widespread defects in homeostasis of the neurovascular unit, including increased intracerebral blood vessels with enhanced perivascular astrogliosis. Additionally, β8 integrin dependent defects in NSC proliferation, survival, and differentiation, as well as neuroblast migration in the RMS were observed both in vivo and in vitro. The defects correlated, in part, with diminished integrin-mediated activation of TGFβ, an ECM ligand of β8 integrin. Collectively, these data identify important adhesion and signaling functions for β8 integrin in the regulation of neural stem and progenitor cells in the SVZ as well as in neuroblast migration along the RMS in the adult brain

Validated determination of NRG1 Ig-like domain structure by mass spectrometry coupled with computational modeling

High resolution hydroxyl radical protein footprinting (HR-HRPF) is a mass spectrometry-based method that measures the solvent exposure of multiple amino acids in a single experiment, offering constraints for experimentally informed computational modeling. HR-HRPF-based modeling has previously been used to accurately model the structure of proteins of known structure, but the technique has never been used to determine the structure of a protein of unknown structure. Here, we present the use of HR-HRPF-based modeling to determine the structure of the Ig-like domain of NRG1, a protein with no close homolog of known structure. Independent determination of the protein structure by both HR-HRPF-based modeling and heteronuclear NMR was carried out, with results compared only after both processes were complete. The HR-HRPF-based model was highly similar to the lowest energy NMR model, with a backbone RMSD of 1.6 Å. To our knowledge, this is the first use of HR-HRPF-based modeling to determine a previously uncharacterized protein structure

Interprofessional Faculty Development: Integration of Oral Health into the Geriatric Diabetes Curriculum, from Theory to Practice.

Georgia Dounis,1 Marcia Ditmyer,2 Susan VanBeuge,3 Sue Schuerman,4 Mildred McClain,1 Kiki Dounis,1,5 Connie Mobley21Department of Clinical Sciences, 2Department of Biomedical Sciences, 3Department of Physiological Nursing, 4Department of Physical Therapy, University of Nevada Las Vegas School of Dental Medicine, Las Vegas, NV, USA; 5Department of Family Medicine, University of Nevada School of Medicine, Reno, NV, USABackground: Health care workforce shortages and an increase demand for health care services by an older demographic challenged by oral–systemic conditions are being recognized across health care systems. Demands are placed on health care professionals to render coordinated delivery of services. Management of oral–systemic conditions requires a trained health care workforce to render interprofessional patient-centered and coordinated delivery of health care services. The purpose of this investigation was to evaluate the effectiveness of an interprofessional health care faculty training program.Methods: A statewide comprehensive type 2 diabetes training program was developed and offered to multidisciplinary health care faculty using innovative educational methods. Video-recorded clinically simulated patient encounters concentrated on the oral–systemic interactions between type 2 diabetes and comorbidities. Post-encounter instructors facilitated debriefing focused on preconceptions, self-assessment, and peer discussions, to develop a joint interprofessional care plan. Furthermore, the health care faculty explored nonhierarchical opportunities to bridge common health care themes and concepts, as well as opportunities to translate information into classroom instruction and patient care.Results: Thirty-six health care faculty from six disciplines completed the pre-research and post-research assessment survey to evaluate attitudes, knowledge, and perceptions following the interprofessional health care faculty training program. Post-training interprofessional team building knowledge improved significantly. The health care faculty post-training attitude scores improved significantly, with heightened awareness of the unique oral–systemic care needs of older adults with type 2 diabetes, supporting an interprofessional team approach to care management. In addition, the health care faculty viewed communication across disciplines as being essential and interprofessional training as being vital to the core curriculum of each discipline. Significant improvement occurred in the perception survey items for team accountability and use of uniform terminology to bridge communication gaps.Conclusion: Attitude, knowledge, and perceptions of health care faculty regarding interprofessional team building and the team approach to management of the oral–systemic manifestations of chronic disease in older adults was improved. Uniform language to promote communication across health professionals, care settings, and caregivers/patients, was noted. Interprofessional team building/care planning should be integrated in core curricula.Keywords: team building, patient-centered care, oral–systemic, older adult

Proteomic analysis of the \u3cem\u3eS. cerevisiae\u3c/em\u3e response to the anticancer ruthenium complex KP1019

Like platinum-based chemotherapeutics, the anticancer ruthenium complex indazolium trans-[tetrachlorobis(1H-indazole)ruthenate(iii)], or KP1019, damages DNA, induces apoptosis, and causes tumor regression in animal models. Unlike platinum-based drugs, KP1019 showed no dose-limiting toxicity in a phase I clinical trial. Despite these advances, the mechanism(s) and target(s) of KP1019 remain unclear. For example, the drug may damage DNA directly or by causing oxidative stress. Likewise, KP1019 binds cytosolic proteins, suggesting DNA is not the sole target. Here we use the budding yeast Saccharomyces cerevisiae as a model in a proteomic study of the cellular response to KP1019. Mapping protein level changes onto metabolic pathways revealed patterns consistent with elevated synthesis and/or cycling of the antioxidant glutathione, suggesting KP1019 induces oxidative stress. This result was supported by increased fluorescence of the redox-sensitive dye DCFH-DA and increased KP1019 sensitivity of yeast lacking Yap1, a master regulator of the oxidative stress response. In addition to oxidative and DNA stress, bioinformatic analysis revealed drug-dependent increases in proteins involved ribosome biogenesis, translation, and protein (re)folding. Consistent with proteotoxic effects, KP1019 increased expression of a heat-shock element (HSE) lacZ reporter. KP1019 pre-treatment also sensitized yeast to oxaliplatin, paralleling prior research showing that cancer cell lines with elevated levels of translation machinery are hypersensitive to oxaliplatin. Combined, these data suggest that one of KP1019’s many targets may be protein metabolism, which opens up intriguing possibilities for combination therapy

The UDP‐GalNAcA biosynthesis genes gna‐gne2 are required to maintain cell envelope integrity and in vivo fitness in multi‐drug resistant Acinetobacter baumannii

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/154438/1/mmi14407-sup-0001-Supinfo.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154438/2/mmi14407.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154438/3/mmi14407_am.pd