Genomic dissection of the 1994 Cronobacter sakazakii outbreak in a French neonatal intensive care unit

Background: Cronobacter sakazakii is a member of the genus Cronobacter that has frequently been isolated from powdered infant formula (PIF) and linked with rare but fatal neonatal infections such as meningitis and necrotising enterocolitis. The Cronobacter MLST scheme has reported over 400 sequence types and 42 clonal complexes; however C. sakazakii clonal complex 4 (CC4) has been linked strongly with neonatal infections, especially meningitis. There have been a number of reported Cronobacter outbreaks over the last three decades. The largest outbreak of C. sakazakii was in a neonatal intensive care unit (NICU) in France (1994) that lasted over 3 months and claimed the lives of three neonates. The present study used whole genome sequencing data of 26 isolates obtained from this outbreak to reveal their relatedness. This study is first of its kind to use whole genome sequencing data to analyse a Cronobacter outbreak. Methods: Whole genome sequencing data was generated for 26 C. sakazakii isolates on the Illumina MiSeq platform. The whole genome phylogeny was determined using Mugsy and RaxML. SNP calls were determined using SMALT and SAMtools, and filtered using VCFtools. Results: The whole genome phylogeny suggested 3 distant clusters of C. sakazakii isolates were associated with the outbreak. SNP typing and phylogeny indicate the source of the C. sakazakii could have been from extrinsic contamination of reconstituted infant formula from the NICU environment and personnel. This pool of strains would have contributed to the prolonged duration of the outbreak, which was up to 3 months. Furthermore 3 neonates were co-infected with C. sakazakii from two different genotype clusters. Conclusion: The genomic investigation revealed the outbreak consisted of an heterogeneous population of C. sakazakii isolates. The source of the outbreak was not identified, but probably was due to environmental and personnel reservoirs resulting in extrinsic contamination of the neonatal feeds. It also indicated that C. sakazakii isolates from different genotype clusters have the ability to co-infect neonates

Terrorism Activities Influence on Financial Stock Markets: An Empirical Evidence from United Kingdom, India, France, Pakistan, Spain and United States

The study investigats the impact of terrorism activities on five economies (Developing and Developed) financial stock markets. Spain, United Kingdom, India, Pakistan, America and France were chosen for the analysis. The variables considered were terrorist activities and market return of the financial stock markets. Daily time series data for the period from 1st Jan 2001 to 31st Dec 2018 was analyzed by applying simple linear regression model to estimate the effects of terrorist activities on financial stock market returns of the selected countries. The results suggest that the market return is affected by the terror events and the model is overall statistically significant. The results of this study are consistent with findings of Freytag et al. (2009) and Basuchoudhary and Shughart (2010). Significant influence of terrorist activities on financial market returns offer financial markets stakeholders not only an understanding of the direction of market swing following terror factors on stock market but also offers guidance towards investment decision making and timing. Study further discussed how terrorism activities influence the overall market return

Draft genome sequence of "Candidatus Cronobacter colletis" NCTC 14934T, a new species in the genus Cronobacter

Members of the Cronobacter genus are associated with serious infections in neonates. This is the first report of the draft genome sequence for the newly proposed species Cronobacter colletis

Draft genome sequences of three newly identified species in the genus Cronobacter, C. helveticus LMG23732T, C. pulveris LMG24059, and C. zurichensis LMG23730T

Cronobacter helveticus, Cronobacter pulveris, and Cronobacter zurichensis are newly described species in the Cronobacter genus, which is associated with serious infections of neonates. This is the first report of draft genome sequences for these species

Draft genome sequence of the earliest Cronobacter sakazakii sequence type 4 strain, NCIMB 8272

The Cronobacter sakazakii clonal lineage defined as sequence type 4 (ST4) is associated with severe cases of neonatal meningitis and persistence in powdered infant formula. For genome sequencing of the earliest deposited culture collection strain of Cronobacter sakazakii ST4, we used the strain NCIMB 8272, originally isolated from milk powder in 1950

The Case Against Commodity Agreements

Depth sensing nano-indentation investigations have been performed to determine the radial dependence of the hardness through the cross section of a Fe-based bulk glassy rod. We have found the hardness of the material decreases along radius from the center to the outermost surface. This phenomenon is attributed to the ‘cooling rate induced surface softening’.  Furthermore, a significant change (~15 %) in elastic modulus is noticed along the radius as well. QC 20120906Hero-

Assessment of Corporate Social Responsibility (CSR) awareness and practices in manufacturing sector of Pakistan

Globalization and mass communication have significantly influenced the socio-economic growth of countries and organizations are under immense pressure to develop their businesses in a more socially responsible way. Consequently, Corporate Social Responsibility (CSR) has emerged as a business development concept. CSR acknowledgement is low in south Asian countries both at state and corporate levels. Pakistan, being a developing country, is no exception and the manufacturing sector, which is the third largest contributing sector to the economy, is facing the challenge of corporate social compliance. This study explores the awareness level amongst employees in the manufacturing sector and also evaluates the practices of CSR activities in these organizations. The study is mainly focused on the textile and automobile sectors and shows greater CSR awareness in the automobile sector than the textile sector; however, there are many organizations where the concept of CSR is unknown. The findings of this research will help organizations in enhancing understanding of CSR amongst employees and will also allow manufacturing industries to improve their implementation against identified weak areas. Increased levels of social compliance will ultimately help organizations in promoting their businesses in the global market

An inversion procedure for the recovery of propagation speed and damping of the medium

We consider the one-dimensional inverse problem of determining variations in propagation speed taking into account damping of the medium. We also consider the inverse problemof recovering variations in damping fromobserv ations of signals which pass through the medium of interest. Our method is based on the linearized inversion associated with Born’s approximation. Thus we assume wave speed and damping are well approximated by the background plus the perturbation. We exploit the high-frequency character of seismic data. Therefore, we use WKBJ Green’s function in deriving our inversion representation