Sequestration of pRb by cyclin D3 causes intranuclear reorganization of lamin A/C during muscle cell differentiation

The A-type lamins that localize in nuclear domains termed lamin speckles are reorganized and antigenically masked specifically during myoblast differentiation. This rearrangement was observed to be linked to the myogenic program as lamin speckles, stained with monoclonal antibody (mAb) LA-2H10, were reorganized in MyoD-transfected fibroblasts induced to transdifferentiate to muscle cells. In C2C12 myoblasts, speckles were reorganized early during differentiation in cyclin D3-expressing cells. Ectopic cyclin D3 induced lamin reorganization in C2C12 myoblasts but not in other cell types. Experiments with adenovirus E1A protein that can bind to and segregate the retinoblastoma protein (pRb) indicated that pRb was essential for the cyclin D3-mediated reorganization of lamin speckles. Cyclin D3-expressing myoblasts displayed site-specific reduction of pRb phosphorylation. Furthermore, disruption of lamin structures by overexpression of lamins inhibited expression of the muscle regulatory factor myogenin. Our results suggest that the reorganization of internal lamins in muscle cells is mediated by key regulators of the muscle differentiation program

Preparation of Al2O3 - SiC In-Situ Composite Powder Mixtures by Carbothermal Reduction of Kaolinite

A1,,O3-SiC,, composite powder mixtures were prepared by carbothermal reduction of kaolinite (A],03.2SiO,.2H,O) in argon atmosphere at constant gas pressure of 0.15 MPa. The reactions were carried out with different mole ratios of silica (in the precursor) and carbon (4.5-9.0), with two types of carbon i.e.carbon black (300m2/g)and activated charcoal (1000m2/g) and at different temperatures in the temperature range of 1550-17000C with one hour of soaking to find the progress of the reaction in all the cases. It was observed that the reactions were complete at 17000C. The reactions were carried out with higher soaking time to optimise the process. It was noted that mullite forms as the intermediate phase at lower temperatures which converts progressively later to a mixture of A1,O, and SiC,, composite product at higher temperatures

Identification of cyclin D3 as a new interaction partner of lamin A/C

Lamin A/C is a major component of the nuclear lamina. An intact nuclear lamina has been proposed to be necessary for muscle differentiation. Cyclin D3 is known to be upregulated in differentiated muscle cells and to form insoluble complexes with cell-cycle regulatory factors in these cells. We have examined the possibility of direct binding interactions between lamin A/C and cyclin D3 by in vitro binding assays and co-immunoprecipitation studies with muscle cells. Our results indicate that cyclin D3 binds specifically to amino acid residues 383–474 of lamin A/C and associates with lamin A/C in muscle cells. The identification of cyclin D3 as a novel binding partner of lamin A/C has important implications for a role for lamin A/C in muscle differentiation

Low level red laser therapy using wavelength 635nm for diabetic foot ulcers: A prospective study

Chronic wounds are an economic burden to the patient as they put them out of work and consume quality working days. Utilization of laser therapy to successfully treat a non-healing ulcer of the lower extremity is profiled. Prior to initiating laser therapy, this particular lesion had continued to increase in dimension. Laser therapy applied over a course of three weeks stimulated granulation tissue formation in the wound bed and re-epithelisation of the ulcer thus enhancing wound healing. Laser therapy is a useful tool to heal ulcers in a short time. Randomized control study to test the efficacy of low level red laser 635nm wavelength on diabetic foot ulcer healing done at RGGGH, Chennai over 7 months period from February 2017 to august 2017. Total of 164 patients studied and divided into study and control groups. Randomized into two groups: Control = 82, Cases = 82. Cases received red laser therapy using wavelength 635nm daily half an hour for 3weeks whereas controls received only saline dressings. Ulcer areas were measured before initiation of therapy and measured at 1st week, 2nd week, 3rd week after initiation of therapy. Datas obtained were entered in Microsoft excel spreadsheet. Datas were entered as rates, percentages and ratios. Comparison was done by using chi square test, unpaired t test. Intra group comparison was done by ANOVA test. P Value equal or less than 0.05 was taken as significant. DFS is more common in males between 55-65yrs. Males to female ratio is 3:1. Trauma is the most common triggering factor. After three weeks of therapy mean reduction in ulcer area in study group as 1044.80±264.09mm2 and 324.26±84.81mm2 In control group. Difference between two group is statistically significant. LLLT for diabetic foot ulcers is an adjunctive therapy can be recommended to all diabetic foot ulcers

Improvement of Weld Bead Characteristics in Gas Metal Arc Welding of SA515 Carbon Steel by Applying Alternating Shielding Gas Flow Technique

High service temperature of pressure vessel components necessitates the use of welded SA515 grade carbon steel components. The gas metal arc welding (GMAW) process using CO2 as shielding gas is known for its undesirable spatter behaviour and inferior weld quality. The alternating shielding gas flow (ASGF) technique is proposed in this study using the shielding gases, viz. CO2 and argon to overcome this difficulty. The welding current, stand-off distance, and shielding gas flow were all varied to improve the bead-on-plate profile geometry. The bead profile parameters such as depth of penetration, bead width, and bead height are considered as weld bead parameters. The following methods are used: correlation analysis, analysis of variance (ANOVA), modelling, and grey relational analysis (GRA). According to the findings, the welding current and ASGF are the most influential parameters impacting the weld bead characteristics. By increasing the welding current, the bead profile parameters increase linearly. The geometry of the bead profile was improved by using the GRA

Flood susceptibility analysis (FSAn) using multi-criteria evaluation (MCE) technique for landuse planning: A case from Penampang, Sabah, Malaysia

Flooding is one of the main natural disasters in Sabah, Malaysia. Several current cases of disastrous flooding were recorded particularly in Penampang area, Sabah (e.g. July 1999; October 2010; April 2013; October & December 2014). Substantial downpour has triggered floods and caused extreme loss in Penampang area. The 2014 floods have affected 40,000 people from 70 villages. The objectives of this research are (i) To determine the factors contributing to the flood occurrences; (ii) To analyst the Flood Susceptibility Level (FSL); and (iii) and to produce the flood hazard map for the study area. In this study, eight (8) parameters were considered in relation to the causative factors to flooding, which are: rainfall, slope gradient, elevation, drainage density, land use, soil textures, slope curvatures and flow accumulation. Flood Susceptibility Analysis (FSAn) map was produced based on the data collected from the field survey, laboratory analysis, high resolution digital radar images (IFSAR) acquisition, and secondary data in year 2014. FSL was defined using Multi Criteria Evaluation (MCE) technique integrated with GIS software. Based on the FSAn, approximately 3.17% of total study area classified as Very High Hazard (VHH), 4.55% as High Hazard (HH), 15.52% as Moderate Hazard (MH), 15.72% as Low Hazard (LH) dan 61.04% as Very Low Hazard (VLH) respectively. Based on the risk rate, requirements for the development procedure has been recommended in this paper. The map produced will be a very useful source for consulting, planning agencies and local governments in managing risk, land-use zoning and redressal efforts to mitigate risks. Besides, the method used in this study can easily be applied to other areas, where other factors may be considered, depending on the convenience of data

Technology-assisted dietary assessment

Dietary intake provides valuable insights for mounting intervention programs for prevention of disease. With growing concern for adolescent obesity, the need to accurately measure diet becomes imperative. Assessment among adolescents is problematic as this group has irregular eating patterns and have less enthusiasm for recording food intake. Preliminary studies among adolescents suggest that innovative use of technology may improve the accuracy of diet information from young people. In this paper, we propose a novel food record method using a mobile device that will provide an accurate account of daily food and nutrient intake among adolescents. Our approach includes the use of image analysis tools for identification and quantification of food consumption. Images obtained before and after food is consumed can be used to estimate the diet of an individual. In this paper we describe our initial results and indicate the potential of the proposed system

Comparison of methods for evaluating the suitability of Vertisols for Gossypium hirsutum (Bt cotton) in two contrasting agro-ecological regions

This is the author accepted manuscript. The final version is available from Taylor and Francis (Routledge) via the DOI in this record.Cotton (Gossypium sp.) is a major crop grown under rainfed conditions in Vertisols and associated soils in semi-arid tropical (SAT) regions of Peninsular India. In recent years, cotton productivity has declined due to various biophysical factors including pest and diseases, seasonal water stress soil degradation and poor crop management practices. In this study, we compare two methods for evaluating the suitability of Vertisols for cotton in contrasting two agro-ecological regions viz., sub-humid moist (SHM) region and semi-arid dry(SAD) were characterized. Twelve cotton growing Vertisols (seven from SHM and five from SAD) were evaluated for their suitability for cotton cultivation using soil quality index (SQI) and modified Sys-FAO method. SQIs were calculated using the weighted additive index from transformed scores of selected indicators by principal component analysis. For Sys-FAO method both biophysical and soil characteristics were considered for suitability evaluation. We found that the soils of SHM region were moderately suitable for cotton cultivation with soil moisture as the major limiting factor, whereas the soils of SAD region are marginally suitable due to high exchangeable sodium percentage and poor hydraulic conductivity. From this, it may be concluded that the weighted SQI has better agreement with the cotton yield

Ultrathin high-resolution flexographic printing using nanoporous stamps

Since its invention in ancient times, relief printing, commonly called flexography, has been used to mass-produce artifacts ranging from decorative graphics to printed media. Now, higher-resolution flexography is essential to manufacturing low-cost, large-area printed electronics. However, because of contact-mediated liquid instabilities and spreading, the resolution of flexographic printing using elastomeric stamps is limited to tens of micrometers. We introduce engineered nanoporous microstructures, comprising polymer-coated aligned carbon nanotubes (CNTs), as a next-generation stamp material. We design and engineer the highly porous microstructures to be wetted by colloidal inks and to transfer a thin layer to a target substrate upon brief contact. We demonstrate printing of diverse micrometer-scale patterns of a variety of functional nanoparticle inks, including Ag, ZnO, WO[subscript 3], and CdSe/ZnS, onto both rigid and compliant substrates. The printed patterns have highly uniform nanoscale thickness (5 to 50 nm) and match the stamp features with high fidelity (edge roughness, ~0.2 μm). We derive conditions for uniform printing based on nanoscale contact mechanics, characterize printed Ag lines and transparent conductors, and achieve continuous printing at a speed of 0.2 m/s. The latter represents a combination of resolution and throughput that far surpasses industrial printing technologies.Massachusetts Institute of Technology. Department of Mechanical EngineeringNational Science Foundation (U.S.) (Grant CMMI-1463181)United States. Air Force Office of Scientific Research. Young Investigator Program (Grant FA9550-11-1-0089)National Institutes of Health (U.S.) (Grant 1R21HL114011-01A1

Conductive Buffer Layers and Overlayers for the Thermal Stability of Coated Conductors

We analyze fundamental issues related to the thermal and electrical stability of a coated conductor during its operation. We address the role of conductive buffer layers in the stability of Ni-based coated conductors, and the effect of a metallic cap layer on the electrical properties of Ni alloy-based superconducting tapes. For the first case we report on the fabrication of a fully conductive RABiTS architecture formed of bilayers of conductive oxides SrRuO3 and LaNiO3 on textured Ni tapes. For the second case we discuss measurements of current-voltage relations on Ag/YBa2Cu3O7-d and Cu/Ag/ YBa2Cu3O7-d prototype multilayers on insulating substrates. Limitations on the overall tape structure and properties that are posed by the stability requirement are presented.Comment: 4 pages, 5 figure