Information and communication technology-based service platform enabling the co-creation of agrometeorological services: A case study of the Laos Climate Services for Agriculture

Climate services for agriculture (agrometeorological services) are only possible through collaborative, interdisciplinary efforts. To this end, a national-scale service operated by centralized governments will mandate institutional coordination for data sharing, in addition to designated staff time and budget commitments for effective and sustainable operation; however, previous efforts have revealed that institutional coordination, particularly between meteorology and agriculture, represents the most difficult part preventing successful implementation in most countries. In this study, we examined the use of a top-down, information and communication technology (ICT)-based multidisciplinary platform for agrometeorological services for facilitating institutional coordination and ensuring project sustainability. The Strengthening Agro-climatic Monitoring and Information Systems (SAMIS) project, operational since 2018, focuses on building the adaptive capacity of agrometeorological services in Laos by combining the agricultural and meteorological sectors. The research here utilized the ICT-based service platform Laos Climate Services for Agriculture (LaCSA) to systematically engage relevant institutional partners in the co-creation process of agrometeorological services. The results showed that LaCSA enabled co-creation, including data sharing, collaborative tool use, institutional coordination, and policy support from multiple government entities across the agricultural and meteorological sectors. Key lessons and suggestions included co-training for both sectors, easy-to-use data management processes, early buy-in for policy makers, and creating standard operating procedures designating staff time and budgets. These results indicate that the coordinated operation of agrometeorological services through an ICT-based service platform can be successfully achieved in Laos as well as other developing countries, where institutional disconnects and human capital constraints hinder successful implementation of agrometeorological services

Activity of the multikinase inhibitor dasatinib against ovarian cancer cells

BackgroundHere, we explore the therapeutic potential of dasatinib, a small-molecule inhibitor that targets multiple cytosolic and membrane-bound tyrosine kinases, including members of the Src kinase family, EphA2, and focal adhesion kinase for the treatment of ovarian cancer.MethodsWe examined the effects of dasatinib on proliferation, invasion, apoptosis, cell-cycle arrest, and kinase activity using a panel of 34 established human ovarian cancer cell lines. Molecular markers for response prediction were studied using gene expression profiling. Multiple drug effect/combination index (CI) isobologram analysis was used to study the interactions with chemotherapeutic drugs.ResultsConcentration-dependent anti-proliferative effects of dasatinib were seen in all ovarian cancer cell lines tested, but varied significantly between individual cell lines with up to a 3 log-fold difference in the IC(50) values (IC(50) range: 0.001-11.3 micromol l(-1)). Dasatinib significantly inhibited invasion, and induced cell apoptosis, but less cell-cycle arrest. At a wide range of clinically achievable drug concentrations, additive and synergistic interactions were observed for dasatinib plus carboplatin (mean CI values, range: 0.73-1.11) or paclitaxel (mean CI values, range: 0.76-1.05). In this study, 24 out of 34 (71%) representative ovarian cancer cell lines were highly sensitive to dasatinib, compared with only 8 out of 39 (21%) representative breast cancer cell lines previously reported. Cell lines with high expression of Yes, Lyn, Eph2A, caveolin-1 and 2, moesin, annexin-1, and uPA were particularly sensitive to dasatinib.ConclusionsThese data provide a clear biological rationale to test dasatinib as a single agent or in combination with chemotherapy in patients with ovarian cancer

Environmental risks from pesticide use: the case of commercial banana farming in northern Lao PDR

Commercial farming of banana for export has rapidly expanded across northern uplands of Laos since 2008 with the establishment of new plantations by foreign companies. Heavy reliance on agrochemical usage warrants examination of possible environmental and human health risks. This study presents a preliminary assessment of the environmental risks from pesticide usage associated with bananas and other major crops in Oudomxay province. Surface water, groundwater, soil and sediment samples collected from the study area were analyzed for pesticide residues in the laboratory during the wet and dry seasons. Results of the analysis revealed that samples from banana farms had higher concentrations of residues from currently used (CU) pesticides compared with samples from adjacent farms producing maize, rubber, upland rice and gourd. Residues from highly persistent organochlorine (OC) pesticides, such as dichlorodiphenyltrichloroethane, heptachlor, dieldrin and lindane, which are no longer used in Laos, were also detected. Laboratory results were compared against a low-cost pesticide residue detection method and a simple pesticide risk assessment tool. However, neither approach was comparable to laboratory analysis. The potential environmental risk from pesticides and pesticide breakdown products was found to be substantial. For example, concentrations of some CU compounds exceeded the limits set by the World Health Organization. The report highlights several mitigation measures to reduce the environmental risks from hazardous pesticides: (i) increase efforts to eliminate the import and use of hazardous and persistent pesticides; (ii) promote targeted education programs to implement best practices, including the selection and use of pesticides as per international standards, and Integrated Pest Management techniques; (iii) identify and protect drinking water sources with a high risk of contamination; and (iv) maintain vegetated buffers and sediment traps to detain farm runoff, which will allow CU pesticides to degrade to safe levels before entering watercourses

A field study on malaria prevalence in southeastern Laos by polymerase chain reaction assay

Abstract. A detection survey for malaria infection by routine microscopy and polymerase chain reaction (PCR) assay was conducted on 336 inhabitants of two villages in Khammouane Province, Lao People’s Democratic Republic (Lao PDR), in July 1997. Malaria infection was demonstrated in 58 (17.3%) subjects by microscopy and in 117 (34.8%) by PCR assay. Specimens positive by both methods were frequent in young villagers, suggesting the presence of many subclinical infections in older persons. The most common species of malaria parasite was Plasmodium falciparum (82.9%). Polymerase chain reaction assay detected mixed infections with 2–4 species in 27 specimens (23.1%). The results demonstrate that there are many subclinical malaria infections with low parasite level and infection with all four human malaria species in Lao PDR

Activity of the multikinase inhibitor dasatinib against ovarian cancer cells.

BackgroundHere, we explore the therapeutic potential of dasatinib, a small-molecule inhibitor that targets multiple cytosolic and membrane-bound tyrosine kinases, including members of the Src kinase family, EphA2, and focal adhesion kinase for the treatment of ovarian cancer.MethodsWe examined the effects of dasatinib on proliferation, invasion, apoptosis, cell-cycle arrest, and kinase activity using a panel of 34 established human ovarian cancer cell lines. Molecular markers for response prediction were studied using gene expression profiling. Multiple drug effect/combination index (CI) isobologram analysis was used to study the interactions with chemotherapeutic drugs.ResultsConcentration-dependent anti-proliferative effects of dasatinib were seen in all ovarian cancer cell lines tested, but varied significantly between individual cell lines with up to a 3 log-fold difference in the IC(50) values (IC(50) range: 0.001-11.3 micromol l(-1)). Dasatinib significantly inhibited invasion, and induced cell apoptosis, but less cell-cycle arrest. At a wide range of clinically achievable drug concentrations, additive and synergistic interactions were observed for dasatinib plus carboplatin (mean CI values, range: 0.73-1.11) or paclitaxel (mean CI values, range: 0.76-1.05). In this study, 24 out of 34 (71%) representative ovarian cancer cell lines were highly sensitive to dasatinib, compared with only 8 out of 39 (21%) representative breast cancer cell lines previously reported. Cell lines with high expression of Yes, Lyn, Eph2A, caveolin-1 and 2, moesin, annexin-1, and uPA were particularly sensitive to dasatinib.ConclusionsThese data provide a clear biological rationale to test dasatinib as a single agent or in combination with chemotherapy in patients with ovarian cancer

Economics of Smallholder Rubber Expansion in Northern Laos

In Northern Laos, as elsewhere in the Southeast Asian uplands, there is an agricultural transition underway from subsistence production based on shifting cultivation to commercial production based on tree crops. In response to demand from China, rubber smallholdings are being established by shifting cultivators in Northern Laos, encouraged by government land-use policy. We examine the bio-economics of smallholder rubber production in an established rubber-growing village and model the likely expansion of smallholder rubber in Northern Laos. Data were obtained from key informants, group interviews, direct observation, and a farm-household survey. Latex yields were estimated using the Bioeconomic Rubber Agroforestry Support System (BRASS). A financial model was developed to estimate the net present value for a representative rubber smallholding. This model was then combined with spatial data in a Geographical Information System (GIS) to predict the likely expansion of rubber based on resource quality and accessibility. Implications are drawn for upland development in the region