Development of high sensitive real-time PCR to detect mustard and other allergens of the family Brassicaceae in food samples

Mustard is a commonly used condiment including in production of other food products. As mustard is an allergen, it is necessary to control its presence. The development of PCR test-systems for its detection is complicated by the fact that this condiment can be made from seeds of various plant species (Brassica juncea, Brassica nigra, Sinapis alba) of the family Brassicaceae that are not closely related. This family includes other plant species such as white cabbage (Brassica oleracea) and rapeseed (Brassica napus), which can cause the allergic reaction, although seldom. In this connection, many authors use primers specific to many species of this family, including to allergens, to detect mustard. In this work, we used the similar strategy. To increase sensitivity, primers for the mitochondrial COX gene were selected. To increase PCR stability in analysis of deeply processed products, primers were selected for a region with a length of 61 base pair. In the work, the specificity and sensitivity of the developed PCR method was confirmed. Analyses of different products, including those that underwent deep technological processing, were carried out with these primers. Also, primers were selected to detect white mustard (S. alba). When analyzing products on the presence of white mustard, charac‑ teristic regional preferences were demonstrated: this species is used in manufacturing products mainly in the UK and USA

Synthesis of Carboxymethyl Starch for increasing drilling mud quality in drilling oil and gas wells

This paper describes the impact of carboxymethyl starch preparation conditions on physicochemical properties of polysaccharide reagent, widely used as fluid loss reducing agent in drilling mud. Variation of the main parameters of carboxymethylation is researched in the experiment. The following conditions such as temperature and reaction time, amount of water, as well as ratio of NaOH to monochloracetic acid define the characteristics of carboxymethyl starch. The degree of substitution is defined for polysaccharides, as well as the characteristics of samples have been studied by infrared spectroscopy. Rheological characteristics and fluid loss indicator have been investigated to study the impact of the reagents on drilling mud quality

The blue vibronically resolved electroluminescence of azatrioxa[8]circulene

Organic Light Emitting Dioides (OLED)devices were fabricated with blue emission based on azatrioxa[8]circulene and 4,4-N,N'-Dicarbazolyl-1,1'-biphenyl (CBP) with maximum brightness of 840 kd/m(2) at 12 V and the starting voltage of 3.5 V. The vibronic emission spectrum was analyzed by the promotive modes calculation method. The electroluminescence of fabricated OLED device is caused by the 0-0 electronic transition and single excitations of 1473 cm(-1) and 1673 cm(-1) modes and combinations thereof.Peer reviewe

Flame front propagation IV: Random Noise and Pole-Dynamics in Unstable Front Propagation II

The current paper is a corrected version of our previous paper arXiv:adap-org/9608001. Similarly to previous version we investigate the problem of flame propagation. This problem is studied as an example of unstable fronts that wrinkle on many scales. The analytic tool of pole expansion in the complex plane is employed to address the interaction of the unstable growth process with random initial conditions and perturbations. We argue that the effect of random noise is immense and that it can never be neglected in sufficiently large systems. We present simulations that lead to scaling laws for the velocity and acceleration of the front as a function of the system size and the level of noise, and analytic arguments that explain these results in terms of the noisy pole dynamics.This version corrects some very critical errors made in arXiv:adap-org/9608001 and makes more detailed description of excess number of poles in system, number of poles that appear in the system in unit of time, life time of pole. It allows us to understand more correctly dependence of the system parameters on noise than in arXiv:adap-org/9608001Comment: 23 pages, 4 figures,revised, version accepted for publication in journal "Combustion, Explosion and Shock Waves". arXiv admin note: substantial text overlap with arXiv:nlin/0302021, arXiv:adap-org/9608001, arXiv:nlin/030201

Tight-binding g-Factor Calculations of CdSe Nanostructures

The Lande g-factors for CdSe quantum dots and rods are investigated within the framework of the semiempirical tight-binding method. We describe methods for treating both the n-doped and neutral nanostructures, and then apply these to a selection of nanocrystals of variable size and shape, focusing on approximately spherical dots and rods of differing aspect ratio. For the negatively charged n-doped systems, we observe that the g-factors for near-spherical CdSe dots are approximately independent of size, but show strong shape dependence as one axis of the quantum dot is extended to form rod-like structures. In particular, there is a discontinuity in the magnitude of g-factor and a transition from anisotropic to isotropic g-factor tensor at aspect ratio ~1.3. For the neutral systems, we analyze the electron g-factor of both the conduction and valence band electrons. We find that the behavior of the electron g-factor in the neutral nanocrystals is generally similar to that in the n-doped case, showing the same strong shape dependence and discontinuity in magnitude and anisotropy. In smaller systems the g-factor value is dependent on the details of the surface model. Comparison with recent measurements of g-factors for CdSe nanocrystals suggests that the shape dependent transition may be responsible for the observations of anomalous numbers of g-factors at certain nanocrystal sizes.Comment: 15 pages, 6 figures. Fixed typos to match published versio

Extended Emission from Short Gamma-Ray Bursts Detected with SPI-ACS/INTEGRAL

The short duration (T90 < 2 s) gamma-ray bursts (GRBs) detected in the SPI-ACS experiment onboard the INTEGRAL observatory are investigated. Averaged light curves have been constructed for various groups of events, including short GRBs and unidentified short events. Extended emission has been found in the averaged light curves of both short GRBs and unidentified short events. It is shown that the fraction of the short GRBs in the total number of SPI-ACS GRBs can range from 30 to 45%, which is considerably larger than has been thought previously.Comment: 27 pages, 10 figure

Existence of radial stationary solutions for a system in combustion theory

In this paper, we construct radially symmetric solutions of a nonlinear noncooperative elliptic system derived from a model for flame balls with radiation losses. This model is based on a one step kinetic reaction and our system is obtained by approximating the standard Arrehnius law by an ignition nonlinearity, and by simplifying the term that models radiation. We prove the existence of 2 solutions using degree theory