Discrimination based on Gly and Arg/Ser at position 673 between dipeptidyl-peptidase (DPP) 7 and DPP11, widely distributed DPPs in pathogenic and environmental gram-negative bacteria

Porphyromonas gingivalis, an asaccharolytic gram-negative rod-shaped bacterium, expresses the novel Asp/Glu-specific dipeptidyl-peptidase (DPP) 11 (Ohara-Nemoto, Y. et al. (2011) J. Biol. Chem. 286, 38115–38127), which has been categorized as a member of the S46/DPP7 family that is preferential for hydrophobic residues at the P1 position. From that finding, 129 gene products constituting five clusters from the phylum Bacteroidetes have been newly annotated to either DPP7 or DPP11, whereas the remaining 135 members, mainly from the largest phylum Proteobacteria, have yet to be assigned. In this study, the substrate specificities of the five clusters and an unassigned group were determined with recombinant DPPs from typical species, i.e., P. gingivalis, Capnocytophaga gingivalis, Flavobacterium psychrophilum, Bacteroides fragilis, Bacteroides vulgatus, and Shewanella putrefaciens. Consequently, clusters 1, 3, and 5 were found to be DPP7 with rather broad substrate specificity, and clusters 2 and 4 were DPP11. An unassigned S. putrefaciens DPP carrying Ser673 exhibited Asp/Glu-specificity more preferable to Glu, in contrast to the Asp preference of DPP11 with Arg673 from Bacteroidetes species. Mutagenesis experiments revealed that Arg673/Ser673 were indispensable for the Asp/Glu-specificity of DPP11, and that the broad specificity of DPP7 was mediated by Gly673. Taken together with the distribution of the two genes, all 264 members of the S46 family could be attributed to either DPP7 or DPP11 by an amino acid at position 673. A more compelling phylogenic tree based on the conserved C-terminal region suggested two gene duplication events in the phylum Bacteroidetes, one causing the development of DPP7 and DPP11 with altered substrate specificities, and the other producing an additional DPP7 in the genus Bacteroides

Effect of plasma shaping on performance in the National Spherical Torus Experiment

The National Spherical Torus Experiment (NSTX) has explored the effects of shaping on plasma performance as determined by many diverse topics including the stability of global magnetohydrodynamic (MHD) modes (e.g., ideal external kinks and resistive wall modes), edge localized modes (ELMs), bootstrap current drive, divertor flux expansion, and heat transport. Improved shaping capability has been crucial to achieving Βt ∼40%. Precise plasma shape control has been achieved on NSTX using real-time equilibrium reconstruction. NSTX has simultaneously achieved elongation κ∼2.8 and triangularity δ∼0.8. Ideal MHD theory predicts increased stability at high values of shaping factor S≡ q95 Ip (a Bt), which has been observed at large values of the S∼37 [MA (m·T)] on NSTX. The behavior of ELMs is observed to depend on plasma shape. A description of the ELM regimes attained as shape is varied will be presented. Increased shaping is predicted to increase the bootstrap fraction at fixed Ip. The achievement of strong shaping has enabled operation with 1 s pulses with Ip =1 MA, and for 1.6 s for Ip =700 kA. Analysis of the noninductive current fraction as well as empirical analysis of the achievable plasma pulse length as elongation is varied will be presented. Data are presented showing a reduction in peak divertor heat load due to increasing in flux expansion. © 2006 American Institute of Physics

Beta-limiting MHD Instabilities in Improved-performance NSTX Spherical Torus Plasmas

Global magnetohydrodynamic stability limits in the National Spherical Torus Experiment (NSTX) have increased significantly recently due to a combination of device and operational improvements. First, more routine H-mode operation with broadened pressure profiles allows access to higher normalized beta and lower internal inductance. Second, the correction of a poloidal field coil induced error-field has largely eliminated locked tearing modes during normal operation and increased the maximum achievable beta. As a result of these improvements, peak beta values have reached (not simultaneously) {beta}{sub t} = 35%, {beta}{sub N} = 6.4, <{beta}{sub N}> = 4.5, {beta}{sub N}/l{sub i} = 10, and {beta}{sub P} = 1.4. High {beta}{sub P} operation with reduced tearing activity has allowed a doubling of discharge pulse-length to just over 1 second with sustained periods of {beta}{sub N} {approx} 6 above the ideal no-wall limit and near the with-wall limit. Details of the {beta} limit scalings and {beta}-limiting instabilities in various operating regimes are described

Stability and Confinement Properties of Auxiliary Heated NSTX Discharges

The National Spherical Torus Experiment (NSTX) is a spherical tokamak with nominal plasma major radius R(subscript ''0'') = 0.85 m, minor radius a = 0.66 m, and aspect ratio A > 1.28. Typical discharge parameters are plasma current I (subscript ''p'') = 0.7-1.4 MA, toroidal magnetic field B(subscript ''t0'') = 0.25-0.45 Tesla at major radius R(subscript ''0''), elongation = 1.7-2.2, triangularity 0.3-0.5, line-average electron density = 2-5 x 10(superscript ''19'') m(superscript ''-3''), electron temperature T(subscript ''e'')(0) = 0.5-1.5 keV, and ion temperature T(subscript ''i'')(0) = 0.5-2 keV. The NSTX auxiliary heating systems can routinely deliver 4.5 MW of 80-keV deuterium neutral beams and 3 MW of 30-MHz high-harmonic fast-wave power. Kinetic profile diagnostics presently include a 10-channel, 30-Hz multipulse Thomson scattering system (MPTS), a 17-channel charge-exchange recombination spectroscopy (CHERS) system, a 48-chord ultra-soft X-ray (USXR) array, and a 15-chord bolometry array. Initial experiments utilizing auxiliary heating on NSTX have focused on MHD stability limits, confinement trends, studying H-mode characteristics, and performing initial power balance calculations

Recent Progress on the National Spherical Torus Experiment (NSTX)

Recent upgrades to the NSTX facility have led to improved plasma performance. Using 5MW of neutral beam injection, plasmas with toroidal {beta}{sub T} (= 2{micro}{sub 0}<p>/B{sub T}{sup 2} where B{sub T} is the vacuum toroidal field at the plasma geometric center) > 30% have been achieved with normalized {beta}{sub N} (= {beta}{sub T}aB{sub I}/I{sub p}) {approx} 6% {center_dot} m {center_dot} T/MA.. The highest {beta} discharge exceeded the calculated no-wall {beta} limit for several wall times. The stored energy has reached 390kJ at higher toroidal field (0.55T) corresponding to {beta}{sub T} {approx} 20% and {beta}{sub N} = 5.4. Long pulse ({approx}1s) high {beta}{sub p} ({approx}1.5) discharges have also been obtained at higher {beta}{sub {phi}} (0.5T) with up to 6MW NBI power. The highest energy confinement times, up to 120ms, were observed during H-mode operation which is now routine. Confinement times of {approx}1.5 times ITER98pby2 for several {tau}{sub E} are observed during both H-Mode and non-H-Mode discharges. Calculations indicate that many NSTX discharges have very good ion confinement, approaching neoclassical levels. High Harmonic Fast Wave current drive has been demonstrated by comparing discharges with waves launched parallel and anti-parallel to the plasma current

Results of NSTX Heating Experiments

The National Spherical Torus Experiment (NSTX) at Princeton is designed to assess the potential of the low-aspect-ratio spherical torus concept for magnetic plasma confinement. The plasma has been heated by up to 5 MW of neutral beam injection, NBI, at an injection energy of 90 keV and up to 6 MW of high harmonic fast wave, HHFW, at 30 MHz. NSTX has achieved beta T of 32%. A variety of MHD phenomena have been observed to limit eta. NSTX has now begun addressing E scaling, eta limits and current drive issues. During the NBI heating experiments, a broad Ti profile with Ti up to 2 keV, Ti > Te and a large toroidal rotation. Transport analysis suggests that the impurity ions have diffusivities approaching neoclassical. For L-Mode plasmas, E is up to two times the ITER-89P L-Mode scaling and exceeds the ITER-98pby2 H-Mode scaling in some cases. Transitions to H-Mode have been observed which result in an approximate doubling of tE. after the transition in some conditions. During HH FW heating, Te > Ti and Te up to 3.5 keV were observed. Current drive has been studied using coaxial helicity injection (CHI), which has produced 390 kA of toroidal current and HHFW, which has produced H-modes with significant bootstrap current fraction at low Ip, high q and high{sub etap}