Continual reproduction of self-assembling oligotriazole peptide nanomaterials.

Autocatalytic chemical reactions, whereby a molecule is able to catalyze its own formation from a set of precursors, mimic nature's ability to generate identical copies of relevant biomolecules, and are thought to have been crucial for the origin of life. While several molecular autocatalysts have been previously reported, coupling autocatalytic behavior to macromolecular self-assembly has been challenging. Here, we report a non-enzymatic and chemoselective methodology capable of autocatalytically producing triskelion peptides that self-associate into spherical bioinspired nanostructures. Serial transfer experiments demonstrate that oligotriazole autocatalysis successfully leads to continual self-assembly of three-dimensional nanospheres. Triskelion-based spherical architectures offer an opportunity to organize biomolecules and chemical reactions in unique, nanoscale compartments. The use of peptide-based autocatalysts that are capable of self-assembly represents a promising method for the development of self-synthesizing biomaterials, and may shed light on understanding life's chemical origins.Molecules that act as both autocatalysts and material precursors offer exciting prospects for self-synthesizing materials. Here, the authors design a triazole peptide that self-replicates and then self-assembles into nanostructures, coupling autocatalytic and assembly pathways to realize a reproducing supramolecular system

The embedded ring-like feature and star formation activities in G35.673-00.847

We present a multi-wavelength study to probe the star formation (SF) process in the molecular cloud linked with the G35.673-00.847 site (hereafter MCG35.6), which is traced in a velocity range of 53-62 km/s. Multi-wavelength images reveal a semi-ring-like feature (associated with ionized gas emission) and an embedded face-on ring-like feature (without the NVSS 1.4 GHz radio emission; where 1-sigma ~ 0.45 mJy/beam) in the MCG35.6. The semi-ring-like feature is originated by the ionizing feedback from a star with spectral type B0.5V-B0V. The central region of the ring-like feature does not contain detectable ionized gas emission, indicating that the ring-like feature is unlikely to be produced by the ionizing feedback from a massive star. Several embedded Herschel clumps and young stellar objects (YSOs) are identified in the MCG35.6, tracing the ongoing SF activities within the cloud. The polarization information from the Planck and GPIPS data trace the plane-of-sky magnetic field, which is oriented parallel to the major axis of the ring-like feature. At least five clumps (having M_clump ~ 740 - 1420 M_sun) seem to be distributed in an almost regularly spaced manner along the ring-like feature and contain noticeable YSOs. Based on the analysis of the polarization and molecular line data, three subregions containing the clumps are found to be magnetically supercritical in the ring-like feature. Altogether, the existence of the ring-like feature and the SF activities on its edges can be explained by the magnetic field mediated process as simulated by Li & Nakamura (2002).Comment: 26 pages, 12 figures, 5 tables. Accepted for publication in The Astrophysical Journa

Embedded filaments in IRAS 05463+2652: early stage of fragmentation and star formation activities

We present a multi-wavelength data analysis of IRAS 05463+2652 (hereafter I05463+2652) to study star formation mechanisms. A shell-like structure around I05463+2652 is evident in the Herschel column density map, which is not associated with any ionized emission. Based on the Herschel sub-millimeter images, several parsec-scale filaments (including two elongated filaments, "s-fl" and "nw-fl" having lengths of ~6.4 pc and ~8.8 pc, respectively) are investigated in I05463+2652 site. Herschel temperature map depicts all these features in a temperature range of ~11-13 K. 39 clumps are identified and have masses between ~70-945 M$_\odot$. A majority of clumps (having M_clump >= 300 M$_\odot$) are distributed toward the shell-like structure. 175 young stellar objects (YSOs) are selected using the photometric 1-5 microns data and a majority of these YSOs are distributed toward the four areas of high column density >= 5 x 10^{21} cm^{-2}; A_V ~5.3 mag) in the shell-like structure, where massive clumps and a spatial association with filament(s) are also observed. The knowledge of observed masses per unit length of elongated filaments and critical mass length reveals that they are supercritical. The filament "nw-fl" is fragmented into five clumps (having M_clump ~100-545 M$_\odot$) and contains noticeable YSOs, while the other filament "s-fl" is fragmented into two clumps (having M_clump ~170-215 M$_\odot$) without YSOs. Together, these observational results favor the role of filaments in star formation process in I05480+2545. This study also reveals the filament "s-fl", containing two starless clumps, at an early stage of fragmentation.Comment: 13 pages, 6 figures, 1 table, Accepted for publication in The Astrophysical Journa

Fluorescent turn-on probes for wash-free mRNA imaging via covalent site-specific enzymatic labeling.

Investigating the many roles RNA plays in cellular regulation and function has increased demand for tools to explore RNA tracking and localization within cells. Our recently reported RNA-TAG (transglycosylation at guanine) approach uses an RNA-modifying enzyme, tRNA-guanine transglycosylase (TGT), to accomplish covalent labeling of an RNA of interest with fluorescent tracking agents in a highly selective and efficient manner. Unfortunately, labeling by this method currently suffers from a high nonspecific fluorescent background and is currently unsuitable for imaging RNA within complex cellular environments. Herein we report the design and synthesis of novel fluorogenic thiazole orange probes that significantly lower nonspecific binding and background fluorescence and, as a result, provide up to a 100-fold fluorescence intensity increase after labeling. Using these fluorogenic labeling agents, we were able to image mRNA expressed in Chinese Hamster Ovary cells in a wash-free manner

Communication and quorum sensing in non-living mimics of eukaryotic cells.

Cells in tissues or biofilms communicate with one another through chemical and mechanical signals to coordinate collective behaviors. Non-living cell mimics provide simplified models of natural systems; however, it has remained challenging to implement communication capabilities comparable to living cells. Here we present a porous artificial cell-mimic containing a nucleus-like DNA-hydrogel compartment that is able to express and display proteins, and communicate with neighboring cell-mimics through diffusive protein signals. We show that communication between cell-mimics allows distribution of tasks, quorum sensing, and cellular differentiation according to local environment. Cell-mimics can be manufactured in large quantities, easily stored, chemically modified, and spatially organized into diffusively connected tissue-like arrangements, offering a means for studying communication in large ensembles of artificial cells

A minimal biochemical route towards de novo formation of synthetic phospholipid membranes.

All living cells consist of membrane compartments, which are mainly composed of phospholipids. Phospholipid synthesis is catalyzed by membrane-bound enzymes, which themselves require pre-existing membranes for function. Thus, the principle of membrane continuity creates a paradox when considering how the first biochemical membrane-synthesis machinery arose and has hampered efforts to develop simplified pathways for membrane generation in synthetic cells. Here, we develop a high-yielding strategy for de novo formation and growth of phospholipid membranes by repurposing a soluble enzyme FadD10 to form fatty acyl adenylates that react with amine-functionalized lysolipids to form phospholipids. Continuous supply of fresh precursors needed for lipid synthesis enables the growth of vesicles encapsulating FadD10. Using a minimal transcription/translation system, phospholipid vesicles are generated de novo in the presence of DNA encoding FadD10. Our findings suggest that alternate chemistries can produce and maintain synthetic phospholipid membranes and provides a strategy for generating membrane-based materials

Perspective on coastal aquaculture in India

The slow growth rate in marine capture fisheries of the coastal sector world over, capital intensive and risk prone deep sea and oceanic harvest coupled with great demand for target groups for domestic and export markets have created a pressing necessity to evolve viable options for aquaculture of most wanted species. Although the aquaculture is rapidly growing in the Asia-Pacific region, its techno-economic and commercial viability in Indian condition is yet to be demonstrated and practiced except in the case of some shrimps and bivalves. Evenlhough the country is blessed with vast cultivable coastal and brackish water areas and with rich potentially cultivable candidate species for farming (20 species of finfishes, 29 crustaceans, 1 7 molluscs, 7 seaweeds and many other species of anciUiary resources), we have yet to go a long way to achieve our targets in aquaculture. The paper reviews the R&D works carried out by CMFRI for crustacean, molluscan, finfish mariculture, seaweed culture and their developmental programmes required to be undertaken in the future

Culture of air-breathing fishes

Though culture of fishes in ponds is one of the age old professions of the world, it is ga in ing prominence, because of the realisation that this source can supply rich and proteinaceous food for human consumption. Majority of the species selected from nature for rearing in ponds belong to the family of crops (Cyprinidae

Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system.

Protein degradation is a fundamental process in all living cells and is essential to remove both damaged proteins and intact proteins that are no longer needed by the cell. We are interested in creating synthetic genetic circuits that function in a cell-free expression system. This will require not only an efficient protein expression platform but also a robust protein degradation system in cell extract. Therefore, we purified and tested the activity of E. coli ClpXP protease in cell-free transcription-translation (TX-TL) systems that used E. coli S30 cell extract. Surprisingly, our studies showed that purified ClpXP added to the TX-TL system has very low proteolytic activity. The low activity of ClpXP was correlated with the rapid consumption of adenosine triphosphate (ATP) in cell extract. We improved the activity of ClpXP in cell extract by adding exogenous ATP and an energy regeneration system. We then established conditions for both protein synthesis, and protein degradation by ClpXP to occur simultaneously in the TX-TL systems. The optimized conditions for ClpXP activity will be useful for creating tunable synthetic genetic circuits and in vitro synthetic biology

Estimates of optimum fleet size for the exploited Indian shelf fisheries

A characteristic feature of marine fish production in India is its annual fluctuations, as vividly shown by the statistics of production for the past four decades. Marine fisheries still remain open access and suffer from overcapitalization. About 2,43,000 fishing vessels (1,82,096 artisanal craft, 26,171 motorised craft and 34,571 mechanised craft) exploit this area, where the estimated annual potential is 2.2 million, tonnes. A conservative estimate of investment on fishing implements (craft as well as gear), at current prices is about Rs. 33.4 billion, but the return per unit investment seems hardly viable. Unhealthy competition and unregulatedfishing may decimate the exploited stocks and therefore, the question of decidingthe optimum size of fishing fleets which wouldallow sustainable yields becomes very relevant