Diversity and Dynamics of Indigenous \u3cem\u3eRhizobium japonicum\u3c/em\u3e Populations

A simple method, based upon the separation of cellular proteins by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, has been devised for distinguishing between isolates of Rhizobium japonicum. Eleven laboratory strains, previously classified into five serogroups, were analyzed by gel electrophoresis. Groups determined subjectively according to protein patterns matched the serogroups, with one exception. Most strains within serogroups could be distinguished from one another. For studying the ecology of Rhizobium, an important advantage of this technique compared with serology or phage typing is that it discriminates among previously unencountered indigenous bacterial isolates as well as among known laboratory strains. SDS-gels were used to analyze the Rhizobium population of 500 nodules, sampled throughout the growing season, from soybeans at two different Wisconsin localities. Although the soybeans had been inoculated with laboratory strains of R. japonicum, indigenous R. japonicum predominated. At one location, 19 indigenous gel types were distinguished and classified mainly into four groups. At the other location, 18 gel types, falling mainly into three groups, were detected. The predominance of a particular group varied, in some cases dramatically, depending upon the time and depth of nodule formation

Innovation Through Social Networking in Communities of Practice

Innovation helps bring competitive advantage to organizations. Without competitive advantage organizations are in danger of not surviving. Two initiatives have recently emerged to support innovation: Communities of Practice (CoPs) and Social Networking. Communities of Practice provide an opportunity to organizations where innovation is created through the knowledge-sharing and informed ways of working. Social Networking is being utilized in organizations to foster collaboration and expose expertise for employees to tap into. The purpose of this study is to determine the value of combining CoPs with social networks. This will be done by studying communities in the MIX (Medtronic Information Exchange) social networking environment. Communities of practice may be inclined to utilize the capabilities of MIX, thus increasing innovation in the organization

Black Hole Thermodynamics and Riemann Surfaces

We use the analytic continuation procedure proposed in our earlier works to study the thermodynamics of black holes in 2+1 dimensions. A general black hole in 2+1 dimensions has g handles hidden behind h horizons. The result of the analytic continuation is a hyperbolic 3-manifold having the topology of a handlebody. The boundary of this handlebody is a compact Riemann surface of genus G=2g+h-1. Conformal moduli of this surface encode in a simple way the physical characteristics of the black hole. The moduli space of black holes of a given type (g,h) is then the Schottky space at genus G. The (logarithm of the) thermodynamic partition function of the hole is the Kaehler potential for the Weil-Peterson metric on the Schottky space. Bekenstein bound on the black hole entropy leads us to conjecture a new strong bound on this Kaehler potential.Comment: 17+1 pages, 9 figure

\u3cem\u3eRhizobium japonicum\u3c/em\u3e Mutants Defective in Symbiotic Nitrogen Fixation

Rhizobium japonicum strains 3I1b110 and 61A76 were mutagenized to obtain 25 independently derived mutants that produced soybean nodules defective in nitrogen fixation, as assayed by acetylene reduction. The proteins of both the bacterial and the plant portions of the nodules were analyzed by two-dimensional polyacrylamide gel electrophoresis. All of the mutants had lower-than-normal levels of the nitrogenase components, and all but four contained a prominent bacteroid protein not observed in wild-type bacteroids. Experiments with bacteria grown ex planta suggested that this protein was derepressed by the absence of ammonia. Nitrogenase component II of one mutant was altered in isoelectric point. The soluble plant fraction of the nodules of seven mutants had very low levels of heme, yet the nodules of five of these seven mutants contained the polypeptide of leghemoglobin. Thus, the synthesis of the globin may not be coupled to the content of available heme in soybean nodules. The nodules of the other two of these seven mutants lacked not only leghemoglobin but most of the other normal plant and bacteroid proteins. Ultrastructural examination of nodules formed by these two mutants indicated normal ramification of infection threads but suggested a problem in subsequent survival of the bacteria and their release from the infection threads

Uniqueness of the asymptotic AdS3 geometry

We explicitly show that in (2+1) dimensions the general solution of the Einstein equations with negative cosmological constant on a neigbourhood of timelike spatial infinity can be obtained from BTZ metrics by coordinate transformations corresponding geometrically to deformations of their spatial infinity surface. Thus, whatever the topology and geometry of the bulk, the metric on the timelike extremities is BTZ.Comment: LaTeX, 8 pages, no figures, version that will appear in Class. Quant. Gra

Anchored Cutoff Structure Design and Construction

As part of a new cogeneration plant being built in Jacksonville, Florida, a 31-foot deep excavation was required to install a 173-foot by 53-foot coal unloading structure in loose to medium-dense fine sands with shallow ground water. A conventional system of excavation support would typically consist of installing and maintaining a dewatering system and driving sheet piles. However, due to the potential for shallow contaminated ground water at the site and a restricted amount of inflow treatment capacity, a nearly complete cutoff or bathtub structure was required. A system that is relatively new to the United States was designed and installed to meet the difficult needs of the site. The system consisted of a sheet pile perimeter wall placed in a cement-bentonite slurry trench, tied back with soil anchors, in conjunction with an anchored six to eight-foot thick soilcrete base mat installed using jet-grouting techniques. This case history provides details regarding design and installation of the anchored cutoff structure. Specifically, design assumptions regarding lateral earth pressures are presented along with predicted versus actual anchor loads for various construction stages. In addition, the results of finite element seepage analysis of the soilcrete base cutoff, and a unique hydrostatic uplift analysis are also presented

A Bayesian analysis of the time through the order penalty in baseball

As a baseball game progresses, batters appear to perform better the more times they face a particular pitcher. The apparent drop-off in pitcher performance from one time through the order to the next, known as the Time Through the Order Penalty (TTOP), is often attributed to within-game batter learning. Although the TTOP has largely been accepted within baseball and influences many managers' in-game decision making, we argue that existing approaches of estimating the size of the TTOP cannot disentangle continuous evolution in pitcher performance over the course of the game from discontinuities between successive times through the order. Using a Bayesian multinomial regression model, we find that, after adjusting for confounders like batter and pitcher quality, handedness, and home field advantage, there is little evidence of strong discontinuity in pitcher performance between times through the order. Our analysis suggests that the start of the third time through the order should not be viewed as a special cutoff point in deciding whether to pull a starting pitcher.Comment: Accepted to JQA

Analytic Continuation for Asymptotically AdS 3D Gravity

We have previously proposed that asymptotically AdS 3D wormholes and black holes can be analytically continued to the Euclidean signature. The analytic continuation procedure was described for non-rotating spacetimes, for which a plane t=0 of time symmetry exists. The resulting Euclidean manifolds turned out to be handlebodies whose boundary is the Schottky double of the geometry of the t=0 plane. In the present paper we generalize this analytic continuation map to the case of rotating wormholes. The Euclidean manifolds we obtain are quotients of the hyperbolic space by a certain quasi-Fuchsian group. The group is the Fenchel-Nielsen deformation of the group of the non-rotating spacetime. The angular velocity of an asymptotic region is shown to be related to the Fenchel-Nielsen twist. This solves the problem of classification of rotating black holes and wormholes in 2+1 dimensions: the spacetimes are parametrized by the moduli of the boundary of the corresponding Euclidean spaces. We also comment on the thermodynamics of the wormhole spacetimes.Comment: 28 pages, 14 figure

Sound propagation in density wave conductors and the effect of long-range Coulomb interaction

We study theoretically the sound propagation in charge- and spin-density waves in the hydrodynamic regime. First, making use of the method of comoving frame, we construct the stress tensor appropriate for quasi-one dimensional systems within tight-binding approximation. Taking into account the screening effect of the long-range Coulomb interaction, we find that the increase of the sound velocity below the critical temperature is about two orders of magnitude less for longitudinal sound than for transverse one. It is shown that only the transverse sound wave with displacement vector parallel to the chain direction couples to the phason of the density wave, therefore we expect significant electromechanical effect only in this case.Comment: revtex, 14 pages (in preprint form), submitted to PR

Postrelease survival, vertical and horizontal movements, and thermal habitats of five species of pelagic sharks in the central Pacific Ocean

From 2001 to 2006, 71 pop-up satellite archival tags (PSATs) were deployed on five species of pelagic shark (blue shark [Prionace glauca]; shortfin mako [Isurus oxyrinchus]; silky shark [Carcharhinus falciformis]; oceanic whitetip shark [C. longimanus]; and bigeye thresher [Alopias superciliosus]) in the central Pacific Ocean to determine species-specific movement patterns and survival rates after release from longline fishing gear. Only a single postrelease mortality could be unequivocally documented: a male blue shark which succumbed seven days after release. Meta-analysis of published reports and the current study (n=78 reporting PSATs) indicated that the summary effect of postrelease mortality for blue sharks was 15% (95% CI, 8.5–25.1%) and suggested that catch-and-release in longline fisheries can be a viable management tool to protect parental biomass in shark populations. Pelagic sharks displayed species-specific depth and temperature ranges, although with significant individual temporal and spatial variability in vertical movement patterns, which were also punctuated by stochastic events (e.g., El Niño-Southern Oscillation). Pelagic species can be separated into three broad groups based on daytime temperature preferences by using the unweighted pair-group method with arithmetic averaging clustering on a Kolmogorov-Smirnov Dmax distance matrix: 1) epipelagic species (silky and oceanic whitetip sharks), which spent >95% of their time at temperatures within 2°C of sea surface temperature; 2) mesopelagic-I species (blue sharks and shortfin makos, which spent 95% of their time at temperatures from 9.7° to 26.9°C and from 9.4° to 25.0°C, respectively; and 3) mesopelagic-II species (bigeye threshers), which spent 95% of their time at temperatures from 6.7° to 21.2°C. Distinct thermal niche partitioning based on body size and latitude was also evident within epipelagic species