Checker of User Experience Guidelines

Cílem této práce je vytvořit nástroj pro automatizované kontrolování správných vlastností uživatelského rozhraní. Součástí práce je seznam pravidel z prostředí GNOME, KDE a OSX, která jsou vhodná k automatické kontrole. Nástroj na pozadí ověřuje prvky uživatelského rozhraní, zatímco uživatel ovládá testovanou aplikaci. Pravidla jsou ohodnocena podle závažnosti a výstupem programu je mimo jiné i výsledné skóre. V závěru práce jsou otestovány běžně dostupné programy. Výsledná aplikace je vhodná pro ověřování grafického uživatelského rozhraní při vývoji.The aim of this work is to create a tool for automated checking the correct characteristics of the user interface. The thesis includes a list of rules from GNOME, KDE and OSX, which are suitable for automatic control. The tool verifies the background elements of the user interface while the user operates the program under test. The rules are ranked according to the severity and outcome of the program is also overall score. The final part contains testing of normally available programs. The application is appropriate for verifying a graphical user interface in development.

A 2D CELLULAR AUTOMATON MODEL OF LIQUID ABSORPTION INTO PAPER FIBERS WITH HYDROPHOBIC TREATMENT

In this work, the issue of applying water or a homogeneous aqueous suspension with a uniform size of (nano)particles (e.g., ink) to the surface of SBSK (southern bleached softwood kraft) paper with randomly arranged local hydrophobic treatment is investigated and then simulated. Based on the two investigated models, various simulation approaches were compared, an own simulation model was created, and its validity was subsequently demonstrated on the experiments performed

IRESite: the database of experimentally verified IRES structures ()

IRESite is an exhaustive, manually annotated non-redundant relational database focused on the IRES elements (Internal Ribosome Entry Site) and containing information not available in the primary public databases. IRES elements were originally found in eukaryotic viruses hijacking initiation of translation of their host. Later on, they were also discovered in 5′-untranslated regions of some eukaryotic mRNA molecules. Currently, IRESite presents up to 92 biologically relevant aspects of every experiment, e.g. the nature of an IRES element, its functionality/defectivity, origin, size, sequence, structure, its relative position with respect to surrounding protein coding regions, positive/negative controls used in the experiment, the reporter genes used to monitor IRES activity, the measured reporter protein yields/activities, and references to original publications as well as cross-references to other databases, and also comments from submitters and our curators. Furthermore, the site presents the known similarities to rRNA sequences as well as RNA–protein interactions. Special care is given to the annotation of promoter-like regions. The annotated data in IRESite are bound to mostly complete, full-length mRNA, and whenever possible, accompanied by original plasmid vector sequences. New data can be submitted through the publicly available web-based interface at and are curated by a team of lab-experienced biologists

Complex network monitoring and management system

Tato diplomová práce se zabývá popisem RM/OSI vrstev, protokolem M-Bus, Wireless M-Bus, SNMP, produkty 2N Telekomunikace a rozborem Raspberry Pi 2 či webovým serveren Apache. Cílem práce je naprogramovat webový konfigurátor s python skripty pro správu daných produktů.This master thesis deals with RM/OSI layers, protocols M-Bus, Wireless M-Bus, SNMP, products 2N Telecomunication and Raspberry Pi 2 with Apache Web server. The goal of the master thesis is to make a web troubleshooting tool with python scripts

Information System for e-Learing Courses Testing

A long-term trend in modern digital education is the shift from static content to interactive e-learning courses. The focus is often on the form of information delivery rather than the content. The testing part of development tends to be ineffective, as there is no easy way to obtain information about student behaviour. This paper addresses this problem by creating a testable web application, xTester, that allows tracking key metrics of developed e-learning courses communicating through xAPI. The solution includes an enhanced course template in Articulate Storyline and a browser add-on to facilitate linking of created courses to the xTester application. General issues and specific stages of development are described, from specification of user requirements, interface design and implementation, to testing. The frontend of the web application is based on the Vue framework, the backend consists of a simple stateless Rest API in Node.js with a SQLite database

TROP2 Represents a Negative Prognostic Factor in Colorectal Adenocarcinoma and Its Expression Is Associated with Features of Epithelial–Mesenchymal Transition and Invasiveness

Trophoblastic cell surface antigen 2 (TROP2) is a membrane glycoprotein overexpressed in many solid tumors with a poor prognosis, including intestinal neoplasms. In our study, we show that TROP2 is expressed in preneoplastic lesions, and its expression is maintained in most colorectal cancers (CRC). High TROP2 positivity correlated with lymph node metastases and poor tumor differentiation and was a negative prognostic factor. To investigate the role of TROP2 in intestinal tumors, we analyzed two mouse models with conditional disruption of the adenomatous polyposis coli (Apc) tumor-suppressor gene, human adenocarcinoma samples, patient-derived organoids, and TROP2-deficient tumor cells. We found that Trop2 is produced early after Apc inactivation and its expression is associated with the transcription of genes involved in epithelial–mesenchymal transition, the regulation of migration, invasiveness, and extracellular matrix remodeling. A functionally similar group of genes was also enriched in TROP2-positive cells from human CRC samples. To decipher the driving mechanism of TROP2 expression, we analyzed its promoter. In human cells, this promoter was activated by β-catenin and additionally by the Yes1-associated transcriptional regulator (YAP). The regulation of TROP2 expression by active YAP was verified by YAP knockdown in CRC cells. Our results suggest a possible link between aberrantly activated Wnt/β-catenin signaling, YAP, and TROP2 expression