Zeytin (olea europea l.) yaprağının antioksidan etkilerinin in vitro yöntemlerle araştırılması

In this study, antioxidant properties of olive (Olea europaea L.) leaves containing oleuropein were investigated in nhexane, ethyl acetate, and methanol based extracts obtained from dried olive leaves at different concentration by various in vitro methods. Percentage yields of the O. europaea were found for the n-hexane extract 13.21%, for ethyl acetate extract 26.15% and for methanol extract 34.59%, respectively. Total phenolic substance content (85.27±15.03%), linoleic acid reduction (89.52±9.77%) and reduction capacity (1.49±0.03) were the highest in the methanol extract. Finally, the DPPH radical scavenging efficiency (72.93±0.42%), reduction of iron (II) ions (50.53±5.53%) and superoxide radical scavenging activity (72.93±0.42%). were the highest in the ethyl acetate extract. In conclusion, it was seen that total phenolic substances, linoleic acid reduction and reduction capacity of the methanol extract of the antioxidant activity of the O. europaea was more active when equated with n-hexane and ethyl acetate extracts. Also, it was found out that the ethyl acetate extract was more effective in DPPH radical scavenging, iron reduction, linoleic acid reduction and superoxide radical scavenging activity. According to the data obtained, it is thought that olive leaf might be evaluated as a natural and cheap alternative antioxidant in different fields such as food, cosmetic, pharmaceutical industry and animal nutrition.Bu çalışmada pek çok sekonder metabolitin yanında major madde olarak oleuropein içeren zeytin (Olea europaea L.) yaprağından elde edilen edilen n-hekzan, etil asetat ve metanol ekstrelerin farklı konsantrasyonlardaki antioksidan aktiviteleri çeşitli in vitro yöntemlerle incelenmiştir. Zeytin yaprağının n-hekzan, etil asetat ve metanollü ekstrelerinin yüzde verimleri sırasıyla %13.21, 26.15 ve %34.59 olarak bulunmuştur. Toplam fenolik madde içeriği (85.27±15.03%), linoleik asit indirgeme (89.52±9.77%) ve indirgenme kapasitesi (1.49±0.03) methanol ekstresinde en yüksek idi. Son olarak, DPPH radikali süpürücü aktivitesi (72.93±0.42%), demir (II) iyonlarını indirgeme (50.53±5.53%) ve süperoksit radikali giderme aktivitesi (72.00±1.35%) etil asetat ekstresinde en yüksekti. Sonuç olarak zeytin yaprağının metanollü ekstresinde toplam fenolik madde, linoleik asit indirgeme ve indirgenme kapasitesi diğer ekstrelere göre daha aktif bulunmuştur. DPPH radikali tutma, demir (II) şelatlama ve süperoksit radikali giderme aktivitesinde ise etil asetat ekstresinin daha etkili olduğu tespit edilmiştir. Elde edilen veriler doğrultusunda, zeytin yaprağının gıda, kozmetik, ilaç endüstrisi ve hayvan besleme gibi alanlarda doğal alternatif bir antioksidan olarak değerlendirilebileceği düşünülmektedir

Evaluation of the Wound Healing Potential of Achillea biebersteinii Afan. (Asteraceae) by In Vivo Excision and Incision Models

Achillea species are widely used for diarrhea, abdominal pain, stomachache and healing of wounds in folk medicine. To evaluate the wound healing activity of the plant, extracts were prepared with different solvents; hexane, chloroform, ethyl acetate and methanol, respectively from the roots of Achillea biebersteinii. Linear incision by using tensiometer and circular excision wound models were employed on mice and rats. The wound healing effect was comparatively evaluated with the standard skin ointment Madecassol. The n-hexane extract treated groups of animals showed 84.2% contraction, which was close to contraction value of the reference drug Madecassol (100%). On the other hand the same extract on incision wound model demonstrated a significant increase (40.1%) in wound tensile strength as compared to other groups. The results of histoptological examination supported the outcome of linear incision and circular excision wound models as well. The experimental data demonstrated that A. biebersteinii displayed remarkable wound healing activity

A Therapeutic Approach for Wound Healing by Using Essential Oils of Cupressus and Juniperus Species Growing in Turkey

Juniperus and Cupressus genera are mainly used as diuretic, stimulant, and antiseptic, for common cold and wound healing in Turkish folk medicine. In the present study, essential oils obtained from cones of Cupressus and berries of Juniperus were evaluated for their wound healing and anti-inflammatory effects. In vivo wound healing activity was evaluated by linear incision and circular excision experimental wound models, assessment of hydroxyproline content, and subsequently histopathological analysis. The healing potential was comparatively assessed with a reference ointment Madecassol. Additionally acetic-acid-induced capillary permeability test was used for the oils' anti-inflammatory activity. The essential oils of J. oxycedrus subsp. oxycedrus and J. phoenicea demonstrated the highest activities, while the rest of the species did not show any significant wound healing effect. The experimental study revealed that J. oxycedrus subsp. oxycedrus and J. phoenicea display remarkable wound healing and anti-inflammatory activities, which support the folkloric use of the plants

Polygala anatolica Boiss. et Heldr.: Is A Potential Remedy for Inflammation and Pain?

Species of Polygala genus have been used for the treatment of inflamation and pain in Turkish traditional medicine. The aim of the present study is to assess the anti-inflammatory and analgesic activities of P. anatolica. n-Hexane, ethyl acetate and methanol extracts of the aerial parts and roots of P. anatolica were investigated for their anti-inflammatory and analgesic effects. The methanol extracts prepared from the aerial parts and roots of P. anatolica were found to be active in carrageenan- and PGE2-induced paw edema models and in Whittle method. Methanolic extract of the aerial part inhibited serotonin-induced hind paw edema, while the root extract did not exert inhibitory effect in the same model. In addition, Fr. B and C obtained from the methanol extract of P. anatolica aerial parts showed significant anti- inflammatory activity. Morover, the analgesic effect of the methanol extracts prepared from the roots and aerial parts and Fr.B and Fr.C were found to be statistically significant without inducing ulceration. The methanol extract obtained from the aerial parts of the plant and its saponoside and flavonoid fractions showed anti-inflammatory and analgesic activities in the trials

Cynara scolymus L. yaprağının farklı ekstraktlarda in vitro aktioksidan aktivitelerinin ve makro ve mikro element seviyelerinin belirlenmesi

Globe Artichoke (Cynara scolymus L.) belonging to the family of Astericaea, has antioxidant, hepatoprotective and hypoglycemic activities, its leaves have traditionally been used for diuretic and choleretic purposes. Therefore the main goal of this study is to determine the total phenolic content of artichoke leaf and its extracts with methanol, ethyl acetate and n- hexane, some in vitro antioxidant activities, selected macro (Na, K, Ca, Mg, P) and micro (Zn, Cu, Mn, Cr, Se, I) elements’ levels. Accordingly the total phenolic content values for the methanol, ethyl acetate and N-hexane extracts found 5,375 mg, 0,917 mg, 0,167 mg Gallic acid (GAE)/g respectively. Methanol extract showed highest DPPH free radical scavenging activity (87,73%), ethyl acetate extract possessed the highest Superoxide radical scavenging activity (SRSA) (49,02 %) whereas N-hexane extract contained high level metal chelating ability ( 289,32µM Fe). In terms of macro and micro elements (except I and Cr levels), the highest concentrations are recorded in its leaves, which are considered as a natural mineral source. Accordingly, it is evaluated that artichoke leaves provide a potential natural sources of K and Zn, while methanol and N-hexane extracts are good sources of P and Zn.Papatyagiller familyasına ait olan Küre enginarın (Cynara scolymus L.) antioksidan, hepatoprotektif ve hipoglisemik etkilere sahiptir, yaprakları geleneksel olarak idrar söktürücü ve koleretik amaçlarla kullanılmaktadır. Bu nedenle bu çalışmanın temel amacı enginar yaprağının ve yaprağın metanollü, etil asetatlı ve n-hekzanlı ekstraktlarının toplam fenolik içeriğini bazı in vitro antioksidan aktiviteleri, seçilmiş makro (Na, K, Ca, Mg, P) ve mikro (Zn, Cu, Mn, Cr, Se, I) element düzeylerini, belirlemektir. Toplam fenolik içerik değerlerine göre metanol, etil asetatlı ve n-hekzan ekstraktları sırasıyla 5,375 mg, 0,917 mg, 0,167 mg Gallik asit (GAE)/g bulunmuştur. Metanol ekstraktı en yüksek DPPH serbest radikal süpürme aktivitesi (%87,73) gösterirken, etil asetat ekstraktı en yüksek süperoksit radikal süpürme aktivitesine (%49,02) sahip iken n-hekzan ekstraktı yüksek seviye metal şelatlama kapasitesi (289,32 µM Fe) içermektedir. Makro ve mikro elementler açısından (I ve Cr seviyeleri hariç) en yüksek konsantrasyonlar doğal mineral kaynağı olarak kabul edilen yapraklarında kaydedilmiştir. Buna göre enginar yapraklarının potansiyel bir doğal K ve Zn kaynağı sağladığı, metanol ve n-hekzan ekstraktlarının ise iyi P ve Zn kaynağı olduğu değerlendirilmektedir

Promising activity of Anthemis austriaca Jacq. on the endometriosis rat model and isolation of its active constituents

© 2019, The Author(s). Heparin and heparan sulfate (Hp/HS) are linear complex glycosaminoglycans which are involved in diverse biological processes. The structural complexity brings difficulties in separation, making the study of structure-function relationships challenging. Here we present a separation method for Hp/HS oligosaccharide fractionation with cross-compatible solvent and conditions, combining size exclusion chromatography (SEC), ion-pair reversed phase chromatography (IPRP), and hydrophilic interaction chromatography (HILIC) as three orthogonal separation methods that do not require desalting or extensive sample handling. With this method, the final eluent is suitable for structure-function relationship studies, including tandem mass spectrometry and microarray printing. Our data indicate that high resolution is achieved on both IPRP and HILIC for Hp/HS isomers. In addition, the fractions co-eluted in IPRP could be further separated by HILIC, with both separation dimensions capable of resolving some isomeric oligosaccharides. We demonstrate this method using both unpurified reaction products from isomeric synthetic hexasaccharides and an octasaccharide fraction from enoxaparin, identifying isomers resolved by this multi-dimensional separation method. We demonstrate both structural analysis by MS, as well as functional analysis by microarray printing and screening using a prototypical Hp/HS binding protein: basic-fibroblast growth factor (FGF2). Collectively, this method provides a strategy for efficient Hp/HS structure-function characterization

The regression of endometriosis with glycosylated flavonoids isolated from Melilotus officinalis (L.) Pall. in an endometriosis rat model

© 2020 Objective: Melilotus officinalis (L.) Pall. is commonly used for treating bronchitis, painful menstruation, hemorrhoids, kidney stones, ulcers of the eyes, earache, and hardening and swelling of uterus. The European Medicines Agency reported the use of M. officinalis orally against stomach ache, gastric ulcer, and disorders of the liver and uterus in folk medicine. The present study aimed to appraise the activity of M. (L.) Pall. aerial parts in endometriosis rat model. Materials and methods: The endometriosis rat model was used to evaluate the potential activity of M. officinalis aerial parts based on its folkloric usage. The aerial parts of M. officinalis were extracted with n-hexane, ethyl acetate (EtOAc), and methanol (MeOH), respectively. The adhesion scores, endometrial foci areas, and cytokine levels were measured in all treated groups. After the biological activity studies, phytochemical studies were performed on the active extract and the fractions obtained from the active extract. Results: The MeOH extract significantly decreased the endometrial foci areas and cytokine levels in rats with endometriosis. Fractionation was performed on the MeOH extract to achieve bioactive molecules. Following the fractionation, the fractions obtained from the MeOH extract were tested. Fraction C showed the highest activity in the rat endometriosis model. Phytochemical investigation of the active fraction (Fraction C) resulted in isolation and elucidation of some quercetin and kaempferol glucoside derivatives. Conclusion: Fraction C obtained from the MeOH extract of M. officinalis showed the highest activity, yielding four glycosylated flavonoids

Original Article Wound Healing Activity of Rubus sanctus Schreber (Rosaceae): Preclinical Study in Animal Models

Young shoots of Rubus species have been used for healing of wounds, infected insect bites and pimples in folk medicine for ages. In order to evaluate the wound healing activity of Rubus sanctus, four different extracts were prepared from the whole aerial parts of the plant by using n-hexane, chloroform, ethyl acetate and methanol, respectively. Incision wound healing model by using tensiometer on rats and excision model on mice were employed to assess the activity. Remarkable wound healing activity was observed with the ointment formulation of the methanol extract at 1% concentration on the mentioned models. The results of histopathological examination also supported the outcome of both incision and excision wound models. The wound healing effect was comparatively evaluated with a reference ointment Madecassol. The experimental data confirmed the ethnobotanical usage of R. sanctus

Ethnopharmacological Approaches to Wound Repair

Wound is breaking of the skin by a physical injury. Wound healing is a connective tissue response along with the repair process which immediately comes after the injury. It occurs as a sequence of phases such as haemostasis, inflammation, proliferation, and remodelling and causes series of interactions between the extracellular matrix, cytokine mediators, and different cell types. For rapid healing several medicinal plants were reported in ethnobotanical studies. Traditional remedies which claimed to have wound healing potential are widely used in developing countries due to their accessibility and low cost. However, these remedies should be evaluated for their efficacy and safety before their utilization. In this context, the papers selected for this special issue include scientifically evaluated information and lead to development of novel drugs for rapid healing of wounds.We would like to thank the authors for their contributions for this special issue. This special issue contains twelve papers. T. Lin et al. investigated the wound healing effect of tocopherol in diabetic rats.This study has proven thewound healing potential of tocopherol cream by increasing the rate of wound closure and total protein content significantly in diabetic condition