16 research outputs found
Figurationen der Weiblichkeit im Zeitalter ihrer technischen Reproduzierbarkeit
Die Reproduktionstechnik, so ließe sich allgemein formulieren, löst das Reproduzierte aus dem Bereich der Tradition ab. Indem sie die Reproduktion vervielfältigt, setzt sie an die Stelle seines einmaligen Vorkommens sein massenweises. Und indem sie der Reproduktion erlaubt, dem Aufnehmenden in seiner jeweiligen Situation entgegenzukommen, aktualisiert sie das Reproduzierte. (Walter Benjamin) Mit Walter Benjamin sind Techniken der Reproduzierbarkeit also zweierlei: Als Techniken der Vervielfäl..
An der Schwelle zur Präsenz: Erleben und Erzählen [Präsenz und Text. Strategien des Transfers in Literatur(wissenschaft) und Philosophie. Interdisziplinärer Workshop, 06.-08.05.2010, Eberhard Karls Universität Tübingen.]
Technik und Gender
Fiktionale Darstellungen von Technik scheinen von Geschlechterstereotypen bevölkert, die Klischees bedienen und auf der Opposition traditioneller, geschlechtlich assoziierter Merkmale basieren. So verkörpert Männlichkeit das technische Prinzip, während das Weibliche der Sphäre der Natur zugeordnet ist. Doch wie stereotyp sind technische Zukunftsvisionen? Der Band nimmt die geschlechtliche Kodiertheit literarischer und filmischer Technikzukünfte in den Fokus. Die Beiträge dieses Sammelbandes betrachten jeweils differenziertere Aspekte, beispielsweise im Hinblick auf stereotype, genderspezifische Rollenzuschreibungen, die Bedeutung geschlechtlicher Identitäten in den von Technik geprägten Räumen und ihre jeweilige mediale Vermittlung, und liefern ein erstes Panoptikum zur Erschließung der Fragestellung von Gender und Technikzukünften unter spezifisch kulturwissenschaftlicher Perspektive
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Large-scale mapping of mutations affecting zebrafish development
BACKGROUND: Large-scale mutagenesis screens in the zebrafish employing the mutagen ENU have isolated several hundred mutant loci that represent putative developmental control genes. In order to realize the potential of such screens, systematic genetic mapping of the mutations is necessary. Here we report on a large-scale effort to map the mutations generated in mutagenesis screening at the Max Planck Institute for Developmental Biology by genome scanning with microsatellite markers. RESULTS: We have selected a set of microsatellite markers and developed methods and scoring criteria suitable for efficient, high-throughput genome scanning. We have used these methods to successfully obtain a rough map position for 319 mutant loci from the Tübingen I mutagenesis screen and subsequent screening of the mutant collection. For 277 of these the corresponding gene is not yet identified. Mapping was successful for 80 % of the tested loci. By comparing 21 mutation and gene positions of cloned mutations we have validated the correctness of our linkage group assignments and estimated the standard error of our map positions to be approximately 6 cM. CONCLUSION: By obtaining rough map positions for over 300 zebrafish loci with developmental phenotypes, we have generated a dataset that will be useful not only for cloning of the affected genes, but also to suggest allelism of mutations with similar phenotypes that will be identified in future screens. Furthermore this work validates the usefulness of our methodology for rapid, systematic and inexpensive microsatellite mapping of zebrafish mutations
Fostering pre-service teachers' technological pedagogical content knowledge (TPACK) : a quasi-experimental field study
Degradation Kinetics of Lignocellulolytic Enzymes in a Biogas Reactor Using Quantitative Mass Spectrometry
The supplementation of lignocellulose-degrading enzymes can be used to enhance the performance of biogas production in industrial biogas plants. Since the structural stability of these enzyme preparations is essential for efficient application, reliable methods for the assessment of enzyme stability are crucial. Here, a mass-spectrometric-based assay was established to monitor the structural stability of enzymes, i.e., the structural integrity of these proteins, in anaerobic digestion (AD). The analysis of extracts of Lentinula edodes revealed the rapid degradation of lignocellulose-degrading enzymes, with an approximate half-life of 1.5 h. The observed low structural stability of lignocellulose-degrading enzymes in AD corresponded with previous results obtained for biogas content. The established workflow can be easily adapted for the monitoring of other enzyme formulations and provides a platform for evaluating the effects of enzyme additions in AD, together with a characterization of the biochemical methane potential used in order to determine the biodegradability of organic substrates.BMELPeer Reviewe
Penetration of normal, damaged and diseased skin--an in vitro study on dendritic core-multishell nanotransporters.
A growing intended or accidental exposure to nanoparticles asks for the elucidation of potential toxicity linked to the penetration of normal and lesional skin. We studied the skin penetration of dye-tagged dendritic core-multishell (CMS) nanotransporters and of Nile red loaded CMS nanotransporters using fluorescence microscopy. Normal and stripped human skin ex vivo as well as normal reconstructed human skin and in vitro skin disease models served as test platforms. Nile red was delivered rapidly into the viable epidermis and dermis of normal skin, whereas the highly flexible CMS nanotransporters remained solely in the stratum corneum after 6h but penetrated into deeper skin layers after 24h exposure. Fluorescence lifetime imaging microscopy proved a stable dye-tag and revealed striking nanotransporter-skin interactions. The viable layers of stripped skin were penetrated more efficiently by dye-tagged CMS nanotransporters and the cargo compared to normal skin. Normal reconstructed human skin reflected the penetration of Nile red and CMS nanotransporters in human skin and both, the non-hyperkeratotic non-melanoma skin cancer and hyperkeratotic peeling skin disease models come along with altered absorption in the skin diseases