Der Staat als Zuhälter

Until the 20th century there were brothels throughout Europe where prostitution was controlled by the state. The study examines factors in the German, French, and Italian parliaments that gave impetus to the abolition of this system. Besides hygienic, moral, and human rights concerns, the discussion also involved questions of security and foreign policy

Is use of the general system justification scale across countries justified? Testing its measurement equivalence

System justification is a widely researched topic in social and political psychology. One major measurement instrument in system justification research is the General System Justification Scale (G-SJS). This scale has been used, among others, for comparisons across social groups in different countries. Such comparisons rely on the assumption that the scale is measurement equivalent. However, this assumption has never been comprehensively tested. Thus, the present two studies assessed the measurement equivalence of the G-SJS following classic measurement equivalence guidelines (i.e., multigroup confirmatory factor analyses) in Study 1 and using a new method for comparing larger numbers of groups in Study 2 (i.e., alignment optimization). In Study 1, we analysed the measurement equivalence in Great Britain (n = 444), Germany (n = 454), and France (n = 463). In Study 2, we used a publicly available dataset consisting of 66 samples from 30 countries (N = 13,495) to again assess the measurement equivalence of the scale. Results indicated (partial) metric equivalence, but not scalar equivalence in both studies. Overall, the studies indicate that mean comparisons across the examined countries are not warranted with the current form of the G-SJS. The scale needs to be revised for valid cross-country comparisons of means

Dynamic contrast in scanning microscopic OCT

While optical coherence tomography (OCT) provides a resolution down to 1 micrometer it has difficulties to visualize cellular structures due to a lack of scattering contrast. By evaluating signal fluctuations, a significant contrast enhancement was demonstrated using time-domain full-field OCT (FF-OCT), which makes cellular and subcellular structures visible. The putative cause of the dynamic OCT signal is ATP-dependent motion of cellular structures in a sub-micrometer range, which provides histology-like contrast. Here we demonstrate dynamic contrast with a scanning frequency-domain OCT (FD-OCT). Given the inherent sectional imaging geometry, scanning FD-OCT provides depth-resolved images across tissue layers, a perspective known from histopathology, much faster and more efficiently than FF-OCT. Both, shorter acquisition times and tomographic depth-sectioning reduce the sensitivity of dynamic contrast for bulk tissue motion artifacts and simplify their correction in post-processing. The implementation of dynamic contrast makes microscopic FD-OCT a promising tool for histological analysis of unstained tissues.Comment: 7 pages, 3 figures, 1 Video available on reques

Engagiert, engagierter... Fernstudent! Die Bedeutung von Ressourcen und Engagement im Fernstudium

Das Fernstudium stellt besondere Anforderungen an die Studierenden. Es fordert eine selbstregulierte Lernstruktur sowie ein Zeitmanagement, die es erlauben, Studium, Familie und Berufstätigkeit miteinander zu vereinen. Zudem sind meist mehrere Jahre zwischen Schule und Studium vergangen. So liegt die Vermutung nahe, dass gerade bei Studierenden an einer Fernuniversität das Engagement (Bakker, Schaufeli, Leiter & Taris, 2008) besonders bedeutsam ist. Für die Betrachtung dieses Sachverhaltes wurde die Utrecht Work Engagement Scale für Studierende (UWES-S) (Schaufeli, Martinez, Pinto, Salanova & Bakker, 2002) herangezogen. Mit deren Übersetzung ins Deutsche wurde das Studienengagement Fernunistudierenden untersucht. Ferner wurde die Grundannahme des Job-Demands-Resources-Modells (JD-R) (Schaufeli & Bakker, 2004; Bakker & Demerouti, 2007), dass Ressourcen einen durch Engagement vermittelten Einfluss auf den Studienerfolg besitzen (Hakanen, Schaufeli & Ahola, 2008), von uns auf den Studienkontext übertragen. Gerade für Fernstudierende interessante und relevante Ressourcen könnten z.B. die wahrgenommene allgemeine und studienkontextbezogene soziale Unterstützung, die Selbstwirksamkeitserwartung und die erlebte Autonomie bei der Einteilung des Studiums sein. In dieser Studie wurden von uns die folgenden Fragestellungen untersucht: Kann die Übersetzung und Anpassung der UWES-S für unseren Kontext als valide bezeichnet werden? Unterscheiden sich Fernstudierende von Studierenden an Präsenzuniversitäten im Engagement (unabhängig vom Alter)? Gibt es zwischen im Fernstudium relevanten Ressourcen und dem Studienerfolg einen Zusammenhang, der durch das Studienengagement vermittelt wird

Advancing Intraoperative Assessment of Urethral Stricture Anatomic Variation: a Prospective Proof-of-concept Study

INTRODUCTION Urethral strictures, particularly those refractory to endoscopic interventions, are commonly treated through open urethroplasty. However, predicting recurrence in homogeneous patient populations remains challenging. METHODS To address this, we developed an intraoperative urethral stricture assessment tool aiming to identify comprehensive risk predictors. The assessment includes detailed parameters on stricture location, length, urethral bed width, spongiosum thickness, obliteration grade, and spongiofibrosis extension. The tool was prospectively implemented in 106 men with anterior one-stage augmentation urethroplasty 04/2020 to 10/2021. RESULTS An intraoperative granular assessment of intricate stricture characteristics is feasible. Comparative analyses revealed significant differences between bulbar and penile strictures. Bulbar strictures exhibited wider urethral beds and thicker spongiosum compared to penile strictures (all P<0.001). The assessment showed marked variations in the degree of obliteration and spongiofibrosis extension. CONCLUSION Our tool aligns with efforts to standardize urethral surgery, providing insights into subtle disease intricacies and enabling comparisons between institutions. Notably, intraoperative assessment may surpass the limitations of preoperative imaging, emphasizing the necessity of intraoperative evaluation. While limitations include a single-institution study and limited sample size, future research aims to refine this tool and determine its impact on treatment strategies, potentially improving long-term outcomes for urethral strictures

Hyperspectral confocal imaging for high-throughput readout and analysis of bio-integrated microlasers

Integrating micro- and nanolasers into live cells, tissue cultures and small animals is an emerging and rapidly evolving technique that offers noninvasive interrogation and labeling with unprecedented information density. The bright and distinct spectra of such lasers make this approach particularly attractive for high-throughput applications requiring single-cell specificity, such as multiplexed cell tracking and intracellular biosensing. The implementation of these applications requires high-resolution, high-speed spectral readout and advanced analysis routines, which leads to unique technical challenges. Here, we present a modular approach consisting of two separate procedures. The first procedure instructs users on how to efficiently integrate different types of lasers into living cells, and the second procedure presents a workflow for obtaining intracellular lasing spectra with high spectral resolution and up to 125-kHz readout rate and starts from the construction of a custom hyperspectral confocal microscope. We provide guidance on running hyperspectral imaging routines for various experimental designs and recommend specific workflows for processing the resulting large data sets along with an open-source Python library of functions covering the analysis pipeline. We illustrate three applications including the rapid, large-volume mapping of absolute refractive index by using polystyrene microbead lasers, the intracellular sensing of cardiac contractility with polystyrene microbead lasers and long-term cell tracking by using semiconductor nanodisk lasers. Our sample preparation and imaging procedures require 2 days, and setting up the hyperspectral confocal microscope for microlaser characterization requires &lt;2 weeks to complete for users with limited experience in optical and software engineering.</p

Hyperspectral confocal imaging for high-throughput readout and analysis of bio-integrated microlasers

Integrating micro- and nanolasers into live cells, tissue cultures and small animals is an emerging and rapidly evolving technique that offers noninvasive interrogation and labeling with unprecedented information density. The bright and distinct spectra of such lasers make this approach particularly attractive for high-throughput applications requiring single-cell specificity, such as multiplexed cell tracking and intracellular biosensing. The implementation of these applications requires high-resolution, high-speed spectral readout and advanced analysis routines, which leads to unique technical challenges. Here, we present a modular approach consisting of two separate procedures. The first procedure instructs users on how to efficiently integrate different types of lasers into living cells, and the second procedure presents a workflow for obtaining intracellular lasing spectra with high spectral resolution and up to 125-kHz readout rate and starts from the construction of a custom hyperspectral confocal microscope. We provide guidance on running hyperspectral imaging routines for various experimental designs and recommend specific workflows for processing the resulting large data sets along with an open-source Python library of functions covering the analysis pipeline. We illustrate three applications including the rapid, large-volume mapping of absolute refractive index by using polystyrene microbead lasers, the intracellular sensing of cardiac contractility with polystyrene microbead lasers and long-term cell tracking by using semiconductor nanodisk lasers. Our sample preparation and imaging procedures require 2 days, and setting up the hyperspectral confocal microscope for microlaser characterization requires &lt;2 weeks to complete for users with limited experience in optical and software engineering.</p

Towards an Ecological Trait-data Standard

Trait-based approaches are widespread throughout ecological research, offering great potential for trait data to deliver general and mechanistic conclusions. Accordingly, a wealth of trait data is available for many organism groups, but, due to a lack of standardisation, these data come in heterogeneous formats. We review current initiatives and infrastructures for standardising trait data and discuss the importance of standardisation for trait data hosted in distributed open-access repositories. In order to facilitate the standardisation and harmonisation of distributed trait datasets, we propose a general and simple vocabulary as well as a simple data structure for storing and sharing ecological trait data. Additionally, we provide an R-package that enables the transformation of any tabular dataset into the proposed format. This also allows trait datasets from heterogeneous sources to be harmonised and merged, thus facilitating data compilation for any particular research focus. With these decentralised tools for trait-data harmonisation, we intend to facilitate the exchange and analysis of trait data within ecological research and enable global syntheses of traits across a wide range of taxa and ecosystems

Interaction of Mitochondrial Polygenic Score and Lifestyle Factors in LRRK2 p.Gly2019Ser Parkinsonism.

peer reviewed[en] BACKGROUND: A mitochondrial polygenic score (MGS) is composed of genes related to mitochondrial function and found to be associated with Parkinson's disease (PD) risk. OBJECTIVE: To investigate the impact of the MGS and lifestyle/environment on age at onset (AAO) in LRRK2 p.Gly2019Ser parkinsonism (LRRK2-PD) and idiopathic PD (iPD). METHODS: We included N = 486 patients with LRRK2-PD and N = 9259 with iPD from the Accelerating Medicines Partnership® Parkinson's Disease Knowledge Platform (AMP-PD), Fox Insight, and a Tunisian Arab-Berber founder population. Genotyping data were used to perform the MGS analysis. Additionally, lifestyle/environmental data were obtained from the PD Risk Factor Questionnaire (PD-RFQ). Linear regression models were used to assess the relationship between MGS, lifestyle/environment, and AAO. RESULTS: Our derived MGS was significantly higher in PD cases compared with controls (P = 1.1 × 10-8 ). We observed that higher MGS was significantly associated with earlier AAO in LRRK2-PD (P = 0.047, β = -1.40) and there was the same trend with a smaller effect size in iPD (P = 0.231, β = 0.22). There was a correlation between MGS and AAO in LRRK2-PD patients of European descent (P = 0.049, r = -0.12) that was visibly less pronounced in Tunisians (P = 0.449, r = -0.05). We found that the MGS interacted with caffeinated soda consumption (P = 0.003, β = -5.65) in LRRK2-PD and with tobacco use (P = 0.010, β = 1.32) in iPD. Thus, patients with a high MGS had an earlier AAO only if they consumed caffeinated soda or were non-smokers. CONCLUSIONS: The MGS was more strongly associated with earlier AAO in LRRK2-PD compared with iPD. Caffeinated soda consumption or tobacco use interacted with MGS to predict AAO. Our study suggests gene-environment interactions as modifiers of AAO in LRRK2-PD