6 research outputs found
Transcriptomic atlas of mushroom development reveals conserved genes behind complex multicellularity in fungi
The evolution of complex multicellularity has been one of the major transitions in the history of life. In contrast to simple multicellular aggregates of cells, it has evolved only in a handful of lineages, including animals, embryophytes, red and brown algae, and fungi. Despite being a key step toward the evolution of complex organisms, the evolutionary origins and the genetic underpinnings of complex multicellularity are incompletely known. The development of fungal fruiting bodies from a hyphal thallus represents a transition from simple to complex multicellularity that is inducible under laboratory conditions. We constructed a reference atlas of mushroom formation based on developmental transcriptome data of six species and comparisons of >200 whole genomes, to elucidate the core genetic program of complex multicellularity and fruiting body development in mushroom-forming fungi (Agaricomycetes). Nearly 300 conserved gene families and >70 functional groups contained developmentally regulated genes from five to six species, covering functions related to fungal cell wall remodeling, targeted protein degradation, signal transduction, adhesion, and small secreted proteins (including effector-like orphan genes). Several of these families, including F-box proteins, expansin-like proteins, protein kinases, and transcription factors, showed expansions in Agaricomycetes, many of which convergently expanded in multicellular plants and/or animals too, reflecting convergent solutions to genetic hurdles imposed by complex multicellularity among independently evolved lineages. This study provides an entry point to studying mushroom development and complex multicellularity in one of the largest clades of complex eukaryotic organisms
Transcriptomic atlas of mushroom development reveals conserved genes behind complex multicellularity in fungi
International audienceThe evolution of complex multicellularity has been one of the major transitions in the history of life. In contrast to simple mul-ticellular aggregates of cells, it has evolved only in a handful of lineages, including animals, embryophytes, red and brown algae, and fungi. Despite being a key step toward the evolution of complex organisms, the evolutionary origins and the genetic underpinnings of complex multicellularity are incompletely known. The development of fungal fruiting bodies from a hyphal thallus represents a transition from simple to complex multicellularity that is inducible under laboratory conditions. We constructed a reference atlas of mushroom formation based on developmental transcriptome data of six species and comparisons of >200 whole genomes, to elucidate the core genetic program of complex multicellularity and fruiting body development in mushroom-forming fungi (Agaricomycetes). Nearly 300 conserved gene families and >70 functional groups contained developmentally regulated genes from five to six species, covering functions related to fungal cell wall remodeling, targeted protein degradation , signal transduction, adhesion, and small secreted proteins (including effector-like orphan genes). Several of these families, including F-box proteins, expansin-like proteins, protein kinases, and transcription factors, showed expansions in Agaricomycetes, many of which convergently expanded in multicellular plants and/or animals too, reflecting convergent solutions to genetic hurdles imposed by complex multicellularity among independently evolved lineages. This study provides an entry point to studying mushroom development and complex multicellularity in one of the largest clades of complex eukaryotic organisms. complex multicellularity | evolution | fungi | comparative genomics | fruiting body development F ungi represent a diverse lineage of complex multicellular organisms with a unique evolutionary history compared with complex multicellular animals, embryophytes, florideophytes, and laminarean brown algae (1-4). Within the fungal kingdom, complex multicellularity is discussed mostly in the context of fruiting bodies, which are found in at least eight independent lin-eages (2), of which the Pezizomycotina (Ascomycota) and the Agaricomycetes (Basidiomycota) contain the vast majority of species. The mushroom-forming fungi (Agaricomycetes) comprise >21,000 species and originated 350 million years ago (5), approximately coinciding with the origin of tetrapods. Fruiting bodies of mushroom-forming fungi have immense importance in agriculture, ecology, and medicine; they represent an important and sustainable food source, with favorable medicinal properties (e.g., antitumor, immunomodulatory) (6). Mushroom-forming fungi share a single origin of fruiting body formation that probably dates to the most recent common ancestor of the Agaricomycetes, Dacrymycetes, and Tremellomycetes (2). Fruiting body development in mushroom-forming fungi has been subject to surprisingly few studies (see, e.g., refs. 7-10), resulting in a paucity of information on the genetic underpinnings of the origins of complex multicellularity in this group (2). During fruiting body development, fungi deploy mechanisms for hypha-to-hypha adhesion, communication (e.g., via Significance Complex multicellularity is a major evolutionary innovation in the history of life. Mushroom-forming fungi (Agaricomycetes) represent one of the most diverse complex multicellular clades, yet the genetic bases and evolutionary origins of their multicellular development are hardly known. We used readouts of gene expression in six species to find genes with a dynamic expression during the development of fruit-ing bodies. Comparisons across species and to 200 fungal genomes identified the gene families with a conserved expression dynamics in multicellular fruiting bodies and their ancient evolutionary origins. These data outline the major multicellularity-related and developmental processes of mushrooms, including the role of transcriptional reprogramming , gene coexpression networks, and alternative splicing , and reveal significant convergence with other complex multicellular lineages
Transcriptomic atlas of mushroom development reveals conserved genes behind complex multicellularity in fungi
A Magyar Belgyógyász Társaság Dél-magyarországi Decentrum 43. Továbbképző Tudományos Ülésén elhangzó előadások rövid összefoglalói
1. Előszó
2. Alsó végtagi oedema ritka oka
3. Diabeteses ketoacidosis változások az utolsó öt évben
4. IGF-2-termelő prostatatumor okozta súlyos hypoglykaemia. Esetismertetés
5. Diabeteses beteg tünetmentes sokér-betegsége. Esetismertetés
6. Egy motorbaleset szövődményei – kardiológiai vonatkozások. Esetismertetés
7. Erectilis diszfunkció és alacsony tesztoszteronszint metabolikus szindróma tüneteit mutató 1-es típusú diabetesben
8. „Egyelőre idiopathiás” AA-amyloidosis. Esetismertetés
9. Anti-TNF-α-terápia mellett kialakuló lupus erythematosus
10. Pancreastumor vagy pancreatitis?
11. Videodenzitometria segítségével vizsgált myocardialis perfúzió eltérései diabetes mellitusban
12. A Clostridium difficile-fertőzések alakulása intézményünk fertőzőosztályán 2011-ig
13. A vesebetegségek felismerhetőségének aránya az eGFR tükrében vizsgálva
14. A gestatiós diabetes mellitus miatt gondozott asszonyok utánvizsgálataival szerzett tapasztalataink
15. Az aortamerevségi (stiffness) index prognosztikus jelentősége acromegaliában
16. Akut máj- és veseelégtelenség ritka oka. Esetismertetés
17. Diabeteses enteropathia, gastroparesis és neuropathia, vagy valami más? Esetismertetés
18. QT-variabilitás és vércukorszint: kimutatható-e összefüggés egészséges szénhidrát-anyagcseréjű egyénekben?
19. Renalis szimpatikus rádiófrekvenciás katéteres ablatio terápiarezisztens hypertoniában
20. A kalcium-anyagcsere útvesztői
21. Humán helminthiasisok: Az ember féregélősködők okozta bántalmainak klinikuma a gyakorlatban
22. Terápiás hatékonyság vizsgálata fokális szegmentális glomerulosclerosisos esetekben
23. Vércukormérés és csúcstechnológia
24. Metformin a terhességben. Áldás vagy átok?
25. Thrombocytopenia és immunhiány
26. Az inzulinkezeléssel járó testsúlynövekedés elkerülése
intenzív inzulinanalóg-kezelési rendszerrel. Esetismertetés
27. Arteria renalis kardiogén eredetű akut thromboemboliás occlusiójának sikeres thrombolyticus kezelése. Esetismertetés
28. Microvascularis vasomotio és vércukorszint: kimutatható-e összefüggés egészséges szénhidrát-anyagcseréjű egyénekben?
29. Fődiagnózis: cardialis decompensatio. Cardialis decompensatio? Esetbemutatás
30. Tíz év tapasztalatai a gestatiós diabetes szűrésében és gondozásában
31. Az autonóm neuropathia prospektív vizsgálata inzulinpumpával kezelt 1-es típusú diabeteses betegekben
32. Vékonybél-kapszulaendoszkópiával szerzett kezdeti tapasztalataink
33. Transrectalis ultrahang (RUH) szerepe a rectum és perirectalis tér kórképeinek diagnosztikájában. Tapasztalatok saját eseteink kapcsán
34. Korszerű inzulinkezelés detemir bázisanalóggal: saját tapasztalataim és a nemzetközi obszervációs vizsgálat (A1chieve®) tükrében
35. Proteinuria mint cardiovascularis rizik